CycLex® Chitotriosidase Fluorometric Assay Kit

CycLex Chitotriosidase Fluorometric Assay Kit.



The CycLex Research Product CycLex Chitotriosidase Fluorometric Assay Kit is used for the quantitative measurement of chitotriosidase activity in serum/plasma, culture supernatant of activated macrophages and other biological samples. It can be used for 100 assays.

Target: CHITO
Product Type: Kit
Size: 100 Assays
Research Area / Disease: Cell Biology
Storage Temperature: -70°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.


  • 10X Chitotriosidase Assay Buffer
  • 50X Fluoro-Substrate
  • Recombinant Chitotriosidase
  • 10X Stop Solution
  • 4-Methylumbelliferone Standard
  • Instruction manual


  1. Hollak, C. E., van Weely, S., van Oers, M. H. and Aerts, J. M. (1994). Marked elevation of plasma chitotriosidase activity. A novel hallmark of Gaucher disease. J. Clin. Invest. 93: 1288.
  2. Renkema, G. H., Boot, R. G., Muijsers,A.O., Donker-Koopman, W. E. and Aerts, J. M. (1995). Purification and characterization of human chitotriosidase, a novel member of the chitinase family of proteins. J. Biol. Chem. 270: 2198.
  3. Boot, R. G., Renkema, G. H., Strijland, A., van Zonneveld, A. J. and Aerts, J. M. (1995). Cloning of a cDNA encoding chitotriosidase, a human chitinase produced by macrophages. J. Biol. Chem. 270: 26252.
  4. Hakala BE, White C, Recklies AD. (1993) Human cartilage gp-39, a major secretory product of articular chondrocytes and synovial cells, is a mammalian member of chitinase protein family. J Biol Chem. 268: 25803.
  5. Renkema GH, Boot RG, Au FL, Donker-Koopman WE, Strijland A, Muijsers AO, Hrebicek M, Aerts JM. (1998) Chitotriosidase, a chitinase, and the 39-kDa human cartilage glycoprotein, a chitin-binding lectin, are homologues of family 18 glycosyl hydrolases secreted by human macrophages. Eur J Biochem. 251: 504.
  6. Aerts, J. M., and Hollak, C. E. (1997) Plasma and metabolic abnormalities in Gaucher’s disease. Baillieres Clin. Haematol. 10: 691.
  7. Guo Y, He W, Boer AM, Wevers RA, de Bruijn AM, Groener JE, Hollak CE, Aerts JM, Galjaard H, van Diggelen OP. (1995) Elevated plasma chitotriosidase activity in various lysosomal storage disorders. J Inher Metab Dis. 18: 717.
  8. Vedder, A.C., Cox-Brinkman, J., Hollak, C.E. et al. (2006) Plasma chitotriosidase in male Fabry patients: A marker for monitoring lipid-laden macrophages and their correction by enzyme replacement therapy. Mol. Genet. Metab. 89: 239.
  9. Grosso, S., Margollicci, M.A., Bargagli, E. et al. (2004) Serum levels of chitotriosidase as a marker of disease activity and clinical stage in sarcoidosis. Scand. J. Clin. Lab. Invest.. 64: 57.
  10. Boot, R.G., van Achterberg, T.A., van Aken, B.E. et al. (1999) Strong induction of members of the chitinase family of proteins in atherosclerosis: chitotriosidase and human cartilage gp-39 expressed in lesion macrophages. Arterioscler. Thromb. Vasc. Biol. 19: 687.
  11. Artieda, M., Cenarro, A., Ganan, A. et al. (2003) Serum chitotriosidase activity is increased in subjects with atherosclerosis disease. Arterioscler. Thromb. Vasc. Biol. 23: 1645.
  12. Labadaridis, J., Dimitriou, E., Costalos, C. et al. (1998) Serial chitotriosidase activity estimations in neonatal systemic candidiasis. Acta Paediatr. 87: 605.
  13. Labadaridis, I., Dimitriou, E., Theodorakis, M. et al. (2005) Chitotriosidase in neonates with fungal and bacterial infections. Arch. Dis. Child Fetal Neonatal Ed. 90: F531.
  14. Barone, R., Simpore, J., Malaguarnera, L., Pignatelli, S. And Musumeci, S. (2003) Plasma chitotriosidase activity in acute Plasmodium falciparum malaria. Clin. Chim. Acta 331: 79.
  15. Di Rosa, M., Musumeci, M., Scuto, A., Musumeci, S. and Malaguarnera, L. (2005) Effect of interferon-gamma, interleukin-10, lipopolysaccharide and tumor necrosis factor-alpha on chitotriosidase synthesis in human macrophages. Clin. Chem. Lab. Med. 43: 499.
  16. Malaguarnera, L.,Musumeci,M., Licata, F., Di Rosa,M.,Messina, A. and Musumeci, S. (2004) Prolactin induces chitotriosidase gene expression in human monocyte-derived macrophages. Immunol. Lett. 94: 57.
  17. Renkema, G. H., Boot, R. G., Strijland, A. Donker-koopman, W. E., van den Berg, M., Muijsers, A. O., and Aerts, J. M. F. G. (1997) Synthesis, sorting, and processing into distinct isoforms of human macrophage chitotriosidase. Eur. J. Biochem. 244: 279
  18. Eiberg H, den Tandt WR. (1997) Assignment of human plasma methylumbelliferyl -tetra-N-acetylchitotetraoside or chitinase to chromosome 19 by a linkage study. Hum Genet. 101: 205
Code CY-1249
Product Type Kit
Size 100 Assays