CycLex® Acidic Mammalian Chitinase Fluorometric Assay Kit
This kit is used for the quantitative measurement of acidic mammalian chitinase (AMCase) activity.
Specifications:
Description
The CycLex Research Product CycLex Acidic Mammalian Chitinase Fluorometric Assay Kit is used for the quantitative measurement of acidic mammalian chitinase (AMCase) activity in a culture supernatant of stimulated pulmonary epithelial cells, several tissue extracts and other biological samples.
| Target: | Chitinase |
|---|---|
| Product Type: | Kit |
| Size: | 100 Assays |
| Research Area / Disease: | Immunology |
| Storage Temperature: | -70°C |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
Components
- AMCase Assay Buffer
- Chitinase Assay Buffer
- Fluoro-Substrate (4-MU-chitotrioside)
- Recombinant AMCase, Enzyme Dilution Buffer
- 4-Methylumbelliferone Standard
References
- Tharanathan, R. N. and Kittur, F. S: Chitin-the undisputed biomolecule of great potential. Crit. Rev. Food Sci. Nutr. 2003; 43: 61.
- Funkhouser JD, Aronson NN Jr: Chitinase family GH18: evolutionary insights from the genomic history of a diverse protein family. BMC Evol Biol 2007; 7: 96-111.
- Bussink AP, Speijer D, Aerts JM, Boot RG: Evolution of mammalian chitinase(-like) members of family 18 glycosyl hydrolases. Genetics 2007; 177: 959-970.
- Boot RG, Blommart EF, Swart E, Ghauharali-van der Vlugt K, Bijl N, Noe C, Place A, Aerts JM: Identification of a novel acidic mammalian chitinase distinct from chitotriosidase. J Biol Chem 2001; 276: 6770-6778.
- Boot RG, Bussink AP, Verhoek M, de Boer PA, Moorman AF, Aerts JM: Marked differences in tissue-specific expression of chitinases in mouse and man. J Histochem Cytochem 2005; 53: 1283-1292.
- Chou YT, Yao S, Czerwinski R, Fleming M, Krykbaev R, Xuan D, Zhou H, Brooks J, Fitz L, Strand J, Presman E, Lin L, Aulabaugh A, Huang X: Kinetic characterization of recombinant human acidic mammalian chitinase. Biochemistry 2006; 45: 4444-4454.
- Boot, R.G., Blommaart, E.F., Swart, E., Ghauharali-van der Vlugt, K., Bijl, N., Moe, C., Place, A. and Aerts, J.M: Identification of a novel acidic mammalian chitinase distinct from chitotriosidase. J. Biol. Chem. 2001; 276: 6770-6778. 8. Boot, R.G., Renkema, G.H., Strijland, A., van Zonneveld, A.J. and Aerts, J.M:Cloning of a cDNA encoding chitotriosidase, a human chitinase produced by macrophages. J. Biol. Chem. 1995; 270: 26252–26256.
- Zhu Z, Zheng T, Homer RJ, Kim YK, Chen NY, Cohn L, Hamid Q, Elias JA: Acidic mammalian chitinase in asthmatic Th2 inflammation and IL-13 pathway activation. Science 2004; 304: 1678-1682
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