CycLex® Acidic Mammalian Chitinase Fluorometric Assay Kit

This kit is used for the quantitative measurement of acidic mammalian chitinase (AMCase) activity.

Specifications:

Description

The CycLex Research Product CycLex Acidic Mammalian Chitinase Fluorometric Assay Kit is used for the quantitative measurement of acidic mammalian chitinase (AMCase) activity in a culture supernatant of stimulated pulmonary epithelial cells, several tissue extracts and other biological samples.

Target: Chitinase
Product Type: Kit
Size: 100 Assays
Research Area / Disease: Immunology
Storage Temperature: -70°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Components

  • AMCase Assay Buffer
  • Chitinase Assay Buffer
  • Fluoro-Substrate (4-MU-chitotrioside)
  • Recombinant AMCase, Enzyme Dilution Buffer
  • 4-Methylumbelliferone Standard

References

  1. Tharanathan, R. N. and Kittur, F. S: Chitin-the undisputed biomolecule of great potential. Crit. Rev. Food Sci. Nutr. 2003; 43: 61.
  2. Funkhouser JD, Aronson NN Jr: Chitinase family GH18: evolutionary insights from the genomic history of a diverse protein family. BMC Evol Biol 2007; 7: 96-111.
  3. Bussink AP, Speijer D, Aerts JM, Boot RG: Evolution of mammalian chitinase(-like) members of family 18 glycosyl hydrolases. Genetics 2007; 177: 959-970.
  4. Boot RG, Blommart EF, Swart E, Ghauharali-van der Vlugt K, Bijl N, Noe C, Place A, Aerts JM: Identification of a novel acidic mammalian chitinase distinct from chitotriosidase. J Biol Chem 2001; 276: 6770-6778.
  5. Boot RG, Bussink AP, Verhoek M, de Boer PA, Moorman AF, Aerts JM: Marked differences in tissue-specific expression of chitinases in mouse and man. J Histochem Cytochem 2005; 53: 1283-1292.
  6. Chou YT, Yao S, Czerwinski R, Fleming M, Krykbaev R, Xuan D, Zhou H, Brooks J, Fitz L, Strand J, Presman E, Lin L, Aulabaugh A, Huang X: Kinetic characterization of recombinant human acidic mammalian chitinase. Biochemistry 2006; 45: 4444-4454.
  7. Boot, R.G., Blommaart, E.F., Swart, E., Ghauharali-van der Vlugt, K., Bijl, N., Moe, C., Place, A. and Aerts, J.M: Identification of a novel acidic mammalian chitinase distinct from chitotriosidase. J. Biol. Chem. 2001; 276: 6770-6778. 8. Boot, R.G., Renkema, G.H., Strijland, A., van Zonneveld, A.J. and Aerts, J.M:Cloning of a cDNA encoding chitotriosidase, a human chitinase produced by macrophages. J. Biol. Chem. 1995; 270: 26252–26256.
  8. Zhu Z, Zheng T, Homer RJ, Kim YK, Chen NY, Cohn L, Hamid Q, Elias JA: Acidic mammalian chitinase in asthmatic Th2 inflammation and IL-13 pathway activation. Science 2004; 304: 1678-1682
Code CY-1248
Product Type Kit
Size 100 Assays
Price
$654.60
Qty: