CycLex® AMPK Kinase Assay Kit

CycLex AMPK Kinase Assay Kit.

Specifications:

Description

The AMPK kinase assay kit can be used to measure the activity of AMPK for 96 assays. Applications of the kit include screening activators or inhibitors of AMPK, evaluating the effects of pharmacological agents on AMPK in vitro or monitoring the effects of purification of AMPK. MBL International’s AMPK kinase assay kit is highly cited.

Bulk pricing available.

Target: AMPK
Product Type: Kinase Assay Kit
Size: 96 Assays
Research Area / Disease: Cell Biology
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Components

Microplate, 10X Wash Buffer, Kinase Buffer, 20X ATP, 20X DTT, Anti-phospho-mouse IRS-1 S789 Monoclonal Antibody (AS-4C4), HRP conjugated Anti-mouse IgG, Substrate Reagent, Stop Solution

Citations

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Bruckbauer A and Zemel MB. Synergistic effects of metformin, resveratrol, and hydroxymethylbutyrate on insulin sensitivity. Diabetes Metab Syndr Obes. 6, 93-102 (2013),

Bruckbauer A and Zemel MB. Synergistic effects of polyphenols and methylxanthines with Leucine on AMPK/Sirtuin-mediated metabolism in muscle cells and adipocytes. PLoS One. 9, e89166 (2014),

Chi-Hsiao Yeh, Tzu-Ping Chen, Yao-ChangWang, Yu-Min Lin,2 and Shu-Wen Fang; AMP-Activated Protein Kinase Activation during Cardioplegia-Induced Hypoxia/Reoxygenation Injury Attenuates Cardiomyocytic Apoptosis via Reduction of Endoplasmic Reticulum Stress. Mediators of Inflammation,2010:130636.2010,

Dan Li, Duan Wang, Yun Wang, Wenhua Ling, Xiang Feng, Min Xia.; Adenosine Monophosphate Activated Protein Kinase Induces Cholesterol Efflux from Macrophage-Derived Foam Cells and Alleviates Atherosclerosis in Apolipoprotein E-Deficient Mice. J Biol Chem. 285: 33499-509, 2010,

Figarola JL and Rahbar S. Small?molecule COH-SR4 inhibits adipocyte differentiation via AMPK activation. Int J Mol Med. 31, 1166-76 (2013),

Greco SJ, Hamzelou A, Johnston JM, Smith MA, Ashford JW, Tezapsidis N.; Leptin boosts cellular metabolism by activating AMPK and the sirtuins to reduce tau phosphorylation and β-amyloid in neurons. Biochem Biophys Res Commun. 414: 170-4. 2011,

Hsu CY and Chuang YL. Changes in energy-regulated molecules in the trophocytes and fat cells of young and old worker honeybees (Apis mellifera). J Gerontol A Biol Sci Med Sci. 69, 955-64 (2014),

Hsu CY and Hu TH. Energy-regulated molecules maintain young status in the trophocytes and fat cells of old queen honeybees. Biogerontology. 15, 389-400 (2014),

Kim JY et al. Anti-obesity efficacy of nanoemulsion oleoresin capsicum in obese rats fed a high-fat diet. Int J Nanomedicine. 9, 301-10 (2014),

Kobashigawa LC et al. Metformin protects cardiomyocyte from doxorubicin induced cytotoxicity through an AMP-activated protein kinase dependent signaling pathway: an in vitro study. PLoS One. 9, e104888 (2014),

Lee MS et al. Effect of high hydrostatic pressure extract of fresh ginseng on adipogenesis in 3T3-L1 adipocytes. J Sci Food Agric. 10, 1002 (2014),

Lee MS, Kim IH, Kim CT, Kim Y.; Reduction of Body Weight by Dietary Garlic Is Associated with an Increase of Uncoupling Protein mRNA Expression and Activation of AMP-Activated Protein Kinase in Diet-Induced Obese Mice. J Nutr. 141: 1947-53. 2011,

Leonardo J. Magnoni, Yoryia Vraskou, Arjan P. Palstra, Josep V. Planas; AMP-Activated Protein Kinase Plays an Important Evolutionary Conserved Role in the Regulation of Glucose Metabolism in Fish Skeletal Muscle Cells. PLoS ONE 7: e31219, 2012,

Liang Y et al. Constitutive activation of AMPK α1 in vascular endothelium promotes high-fat diet-induced fatty liver injury: role of COX-2 induction. Br J Pharmacol. 171, 498-508 (2014),

Magnoni LJ et al. Fueling the engine: induction of AMP-activated protein kinase in trout skeletal muscle by swimming. J Exp Biol. 217, 1649-52 (2014),

Miyokawa-Gorin K, Takahashi K, Handa K, Kitahara A, Sumitani Y, Katsuta H, Tanaka T, Nishida S, Yoshimoto K, Ohno H, Ishida H.; Induction of mitochondrial uncoupling enhances VEGF(120) but reduces MCP-1 release in mature 3T3-L1 adipocytes: Possible regulatory mechanism through endogenous ER stress and AMPK-related pathways. Biochem Biophys Res Commun. 419: 200-5. 2012,

Noda K et al. Rho-kinase inhibition ameliorates metabolic disorders through activation of AMPK pathway in mice. PLoS One. 9, e110446 (2014),

Park H et al. Consensus scoring approach to identify the inhibitors of AMP-activated protein kinase α2 with virtual screening. J Chem Inf Model. 54, 2139-46 (2014),

Sato S et al. Elevated levels of TWEAK in skeletal muscle promote visceral obesity, insulin resistance, and metabolic dysfunction. FASEB J. 29, 988-1002 (2015),

Seo JY and Cresswell P. Viperin regulates cellular lipid metabolism during human cytomegalovirus infection. PLoS Pathog. 9, e1003497 (2013),

Soga M et al. The di-peptide Trp-His activates AMP-activated protein kinase and enhances glucose uptake independently of insulin in L6 myotubes. FEBS Open Bio. 4, 898-904 (2014),

Takikawa M et al. 10-Hydroxy-2-decenoic acid, a unique medium-chain fatty acid, activates 5′-AMP-activated protein kinase in L6 myotubes and mice. Mol Nutr Food Res. 57, 1794-802 (2013),

Tang YC, Williams BR, Siegel JJ, Amon A.; Identification of aneuploidy-selective antiproliferation compounds. Cell. 144: 499-512. 2011,

Vingtdeux V, Chandakkar P, Zhao H, d’Abramo C, Davies P, Marambaud P.; Novel synthetic small-molecule activators of AMPK as enhancers of autophagy and amyloid-β peptide degradation. FASEB J. 25: 219-31, 2011,

Wang Z, Pini M, Yao T, Zhou Z, Sun C, Fantuzzi G, Song Z.; Homocysteine suppresses lipolysis in adipocytes by activating the AMPK pathway. Am J Physiol Endocrinol Metab. 301: E703-12. 2011,

Yamada S et al. AMP-activated protein kinase-mediated glucose transport as a novel target of tributyltin in human embryonic carcinoma cells. Metallomics. 5, 484-91 (2013),

Yeh CH, Chen TP, Wang YC, Lin YM, Fang SW.; AMP-activated protein kinase activation during cardioplegia-induced hypoxia/reoxygenation injury attenuates cardiomyocytic apoptosis via reduction of endoplasmic reticulum stress. Mediators Inflamm. 2010;2010:130636.,

Ying Wang, Masahiro Nishi, Asako Doi, Takeshi Shono, Yasushi Furukawa, Takeshi Shimada, Hiroto Furuta, Hideyuki Sasaki and Kishio Nanjo; Ghrelin inhibits insulin secretion through the AMPK_UCP2 pathway in β cells. FEBS Lett. 584: 1503-1508, 2010,

Yokoyama Y, Iguchi K, Usui S, Hirano K.; AMP-activated protein kinase modulates the gene expression of aquaporin 9 via forkhead box a2. Arch Biochem Biophys.515:80-8. 2011,

Yu-yi Lin, Samara Kiihl, Yasir Suhail, Shang-Yun Liu, Yi-hsuan Chou, Zheng Kuang, Jin-ying Lu, Chin Ni Khor, Chi-Long Lin, Joel S. Bader, Rafael Irizarry & Jef D. Boeke; Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK. Nature 482, 251_255, 2012,

Zadra G et al. A novel direct activator of AMPK inhibits prostate cancer growth by blocking lipogenesis. EMBO Mol Med. 6, 519-38 (2014),

Zadra G et al. A novel direct activator of AMPK inhibits prostate cancer growth by blocking lipogenesis. EMBO Mol Med. 6, 519-38 (2014),

Zhang X et al. Increased 4-hydroxynonenal formation contributes to obesity-related lipolytic activation in adipocytes. PLoS One. 8, e70663 (2013)

Sumide K et al. A revised road map for the commitment of human cord blood CD34-negative hematopoietic stem cells. Nat Commun. 9, 2202 (2018)

Mok SWF et al. A Method for Rapid Screening of Anilide-Containing AMPK Modulators Based on Computational Docking and Biological Validation. Front Pharmacol. 9, 710 (2018)

Lee MS, Kim Y. Effects of Isorhamnetin on Adipocyte Mitochondrial Biogenesis and AMPK Activation. Molecules 23, E1853 (2018)

Dai SN et al. Ginsenoside Rb1 Ameliorates Autophagy of Hypoxia Cardiomyocytes from Neonatal Rats via AMP-Activated Protein Kinase Pathway. Chin J Integr Med. (2018) In press.

Kawasumi K et al. Changes in Plasma Metabolites Concentrations in Obese Dogs Supplemented With Anti-oxidant Compound. Front Nutr. 5, 74 (2018)

Kim S et al. Ginger Extract Ameliorates Obesity and Inflammation via Regulating MicroRNA-21/132 Expression and AMPK Activation in White Adipose Tissue. Nutrients. 10, 1567 (2018)

Choi S et al. Comparison of pressure- and volume-controlled ventilation during laparoscopic colectomy in patients with colorectal cancer. Sci Rep. 9, 17007 (2019)

References

Sim ATR, Hardie DG. The low activity of acetyl-CoA carboxylase in basal and glucagon-stimulated hepatocytes is due to phosphorylation by the AMP-activated protein kinase and not cyclic AMP-dependent protein kinase. FEBS Lett. 233: 294 –298, 1988

Bolster DR, Crozier SJ, Kimball SR, Jefferson LS. AMP-activated protein kinase suppresses protein synthesis in rat skeletal muscle through downregulated mTOR signaling. J Biol Chem. 277: 23977–23980, 2002

Kawaguchi T, Osatomi K, Yamashita H, Kabashima T, Uyeda K. Mechanism for fatty acids sparing effect on glucose-induced transcription: regulation of carbohydrate response element binding protein by AMP-activated protein kinase. J Biol Chem. 277: 3829 –3835, 2001

Yang W, Hong YH, Shen XQ, Frankowski C, Camp HS, Leff T. Regulation of transcription by AMP-activated Protein Kinase. Phosphorylation of p300 blocks its interaction with nuclear receptors. J Biol Chem. 276: 38341–38344, 2001

Hardie, D.G. The AMP-activated protein kinase pathway: new players upstream and downstream. J. Cell Sci. 117:5479–5487, 2004

Stapleton, D., Woollatt, E., Mitchelhill, K. I., Nicholl, J. K., Fernandez, C. S., Michell, B. J., Witters, L. A., Power, D. A., Sutherland, G. R. and Kemp, B. E.; AMP activated protein kinase isoenzyme family: subunit structure and chromosomal location. FEBS Lett. 409: 452–456, 1997

Kemp, B. E., Stapleton, D., Campbell, D. J., Chen, Z. P., Murthy, S., Walter, M., Gupta, A., Adams, J. J., Katsis, F., van Denderen, B. et al.; AMP-activated protein kinase, super metabolic regulator. Biochem. Soc. Trans. 31: 162–168, 2003

Cheung, P. C. F., Salt, I. P., Davies, S. P., Hardie, D. G. and Carling, D.; Characterization of AMP-activated protein kinase gamma-subunit isoforms and their role in AMP binding. Biochem. J. 346: 659–669, 2000

Hardie, D. G. and Carling, D.; The AMP-activated protein kinase – fuel gauge of the mammalian cell? Eur. J. Biochem. 246: 259–273, 1997

Stapleton, D., Mitchelhill, K. I., Gao, G., Widmer, J., Michell, B. J., Teh, T., House, C. M., Fernandez, C. S., Cox, T., Witters, L. A. and Kemp, B. E.; Mammalian AMP-activated protein kinase subfamily. J. Biol. Chem. 271: 611–614, 1996

Woods, A., Azzout-Marniche, D., Foretz, M., Stein, S. C., Lemarchand, P., Ferre, P., Foufelle, F. and Carling, D.; Characterization of the role of AMP-activated protein kinase in the regulation of glucose-activated gene expression using constitutively active and dominant negative forms of the kinase. Mol. Cell. Biol. 20: 6704–6711, 2000

da Silva Xavier, G., Leclerc, I., Salt, I. P., Doiron, B., Hardie, D. G., Kahn, A. and Rutter, G. A. ; Role of AMP-activated protein kinase in the regulation by glucose of islet beta-cell gene expression. Proc. Natl. Acad. Sci. U.S.A. 97: 4023–4028, 2000

Salt, I. P., Johnson, G., Ashcroft, S. J. and Hardie, D. G.; AMP-activated protein kinase is activated by low glucose in cell lines derived from pancreatic beta cells, and may regulate insulin release. Biochem. J. 335: 533–539, 1998

Code CY-1182
Product Type Kinase Assay Kit
Size 96 Assays
Price
$655.99
Qty: