CycLex® IKKα and β Kinase Assay/Inhibitor Screening Kit

CycLex IKKα/β Kinase Assay/Inhibitor Screening Kit.

Specifications:

Description

The CycLex Research Products IKK α/β assay/Inhibitor Screening Kit is designed to measure the activities of purified IKKα and IKKβ for the rapid and sensitive evaluation of inhibitors or activators. It can be used for 96 Assays.

Target: IKKα and β Kinase
Product Type: Kinase Assay Kit
Size: 96 Assays
Research Area / Disease: Cancer
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Components

  • Microplate
  • 10X Wash Buffer
  • Kinase Buffer
  • 20X ATP
  • Anti-Phospho-IkappaBbeta S32 Monoclonal Antibody (AS-2E8)
  • HRP conjugated Anti-mouse IgG
  • Substrate Reagent
  • Stop Solution

Citations

  1. Fakhrudin N et al. Identification of plumericin as a potent new inhibitor of the NF-κB pathway with anti-inflammatory activity in vitro and in vivo. Br J Pharmacol. 171, 1676-86 (2014),
  2. Harada S, Tominari T, Matsumoto C, Hirata M, Takita M, Inada M, Miyaura C.; Nobiletin, a polymethoxy flavonoid, suppresses bone resorption by inhibiting NFκB-dependent prostaglandin E synthesis in osteoblasts and prevents bone loss due to estrogen deficiency. J Pharmacol Sci. 115: 89-93, 2011,
  3. Heyninck K et al. Withaferin A inhibits NF-kappaB activation by targeting cysteine 179 in IKKβ. Biochem Pharmacol. 91, 501-9 (2014),
  4. Qiu J et al. Hyperammonemia in cirrhosis induces transcriptional regulation of myostatin by an NF-κB-mediated mechanism. Proc Natl Acad Sci U S A. 110, 18162-7 (2013)
  5. Hirata N et al. Beta-Cryptoxanthin Inhibits Lipopolysaccharide-Induced Osteoclast Differentiation and Bone Resorption via the Suppression of Inhibitor of NF-κB Kinase Activity. Nutrients. 11, pii: E368 (2019)

References

  1. Ghosh, S., and Karin, M. (2002) Cell 109, S81–S96
  2. Schmitz, L. M., Bacher, S., and Kracht, M. (2001) Trends Biochem. Sci. 26, 186–190
  3. Naumann, M., and Scheidereit, C. (1994) EMBO J. 13, 4597–4607
  4. Brown, K., Gerstberger, S., Carlson, L., Franzoso, G., and Siabenlist, U. (1995) Science 267, 1485–1488
  5. Chen, Z., Haglen, J., Palombello, V. J., Melandri, F., Scherer, D., Ballard, D., and Maniatis, T. (1995) Genes Dev. 9, 1586–1597
  6. DiDonato, J., Mercurio, F., Rosette, C., Wi-Li, J., Suyang, H., Ghosh, S., and Karin, M. (1996) Mol. Cell. Biol. 16, 1295–1304
  7. Alkalay, I., Yaron, A., Hatzubai, A., Jung, S., Avraham, A., Gerlitz, O., Pashut-Levon, I., and Ben-Neriah, Y. (1995) Mol. Cell. Biol. 15, 1294–1301
  8. Karin, M., and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. 18, 621–663
  9. DiDonato, J. A., Mercurio, F., and Karin, M. (1995) Mol. Cell. Biol. 15, 1302–1311
  10. Scherer, D. C., Brockman, J., Chen, Z., Maniatis, T., and Ballard, D. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 11259–11263
  11. Spencer, E., Jiang, J., and Chen, Z. J. (1999) Genes Dev. 13, 284–294
  12. Zandi, E., Rothwarf, D. M., Delhase, M., Hayakawa, M., and Karin, M. (1997) Cell 91, 243–252
  13. Hu, M. C., and Wang, Y. (1998) Gene (Amst.) 222, 31–40
  14. DiDonato, J. A., Hayakawa, M., Rothwarf, D. M., Zandi, E., and Karin, M. (1997) Nature 388, 548–554
  15. Mercurio, F., Zhu, H., Murray, B. W., Shevchenko, A., Bennet, B. L., and Rao, A. (1997) Science 278, 860–866
  16. Rothwarf, D. M., Zandi, E., Natoli, G., and Karin, M. (1998) Nature 395, 297–300
  17. Yamaoka, S., Courtois, G., Bessia, C., Whiteside, S. T., Weil, R., Agou, F., Kirk, H. E., Kay, R. J., and Israel, A. (1998) Cell 93, 1231–1240
  18. Li, Q., Van Antwerp, D., Mercurio, F., Lee, K. F., and Verma, I. M. (1999) Science 284, 321–32535.
  19. Li, Z. W., Chu, W., Hu, Y., Delhase, M., Deernick, T., Ellisman, M., Johnson, R., and Karin, M. (1999) J. Exp. Med. 189, 1839–1845
  20. Peters, R. T.; Liao, S.-M. (2000) Maniatis, T. .Mol. Cell 5: 513-522
Code CY-1178
Product Type Kinase Assay Kit
Size 96 Assays
Price
$654.60
Qty: