CycLex® Aurora Family Kinase Assay/Inhibitor Screening Kit (DISCONTINUED) (Discontinued)

CycLex Aurora Family Kinase Assay/Inhibitor Screening Kit.

Specifications:

Description

We are sorry for the inconvenience; this product code has been discontinued.

We are sorry for the inconvenience; this product code has been discontinued.

The CycLex Aurora Family Kinase Assay/Inhibitor Screening Kit is designed to measure the activity of purified Aurora family kinases (Aurora A, B, and C) for the rapid and sensitive evaluation of Aurora kinase inhibitors or activators It can be used for 96 assays.

Target: Aurora
Product Type: Kinase Assay Kit
Size: 96 Assays
Research Area / Disease: Cell Biology
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Components

  • Microplate
  • 10x Wash Buffer
  • Kinase Buffer
  • 20x ATP
  • HRP conjugated Detection Antibody
  • Substrate Reagent
  • Stop Solution

References

  1. Francisco, L., Wang, W., and Chan, C.S. (1994). Type 1 protein phosphatase acts in opposition to IpL1 protein kinase in regulating yeast chromosome segregation. Mol. Cell Biol. 14, 4731–4740.
  2. Leverson, J.D., Huang, H.K., Forsburg, S.L., and Hunter, T. (2002). The Schizosaccharomyces pombe aurora-related kinase Ark1 interacts with the inner centromere protein Pic1 and mediates chromosome segregation and cytokinesis. Mol. Biol. Cell 13, 1132–1143.
  3. Adams, R.R., Carmena, M., and Earnshaw, W.C. (2001a). Chromosomal passengers and the (aurora) ABCs of mitosis. Trends Cell Biol. 11, 49–54.
  4. Nigg, E.A. (2001). Mitotic kinases as regulators of cell division and its checkpoints. Nat. Rev. Mol. Cell Biol. 2, 21–32.
  5. Kimura, M., Kotani, S., Hattori, T., Sumi, N., Yoshioka, T., Todokoro, K., and Okano, Y. (1997). Cell cycle-dependent expression and spindle pole localization of a novel human protein kinase, Aik, related to Aurora of Drosophila and yeast Ipl1. J. Biol. Chem. 272, 13766–13771.
  6. Tsai, M.Y., Wiese, C., Cao, K., Martin, O., Donovan, P., Ruderman, J., Prigent, C., and Zheng, Y. (2003). A Ran signaling pathway mediated by the mitotic kinase Aurora A in spindle assembly. Nat. Cell Biol. 5, 242–248.
  7. Terada, Y., Tatsuka, M., Suzuki, F., Yasuda, Y., Fujita, S., and Otsu, M. (1998). AIM-1: a mammalian midbody-associated protein required for cytokinesis. EMBO J. 17, 667–676.
  8. Kimura, M., Matsuda, Y., Yoshioka, T., and Okano, Y. (1999). Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3. J. Biol. Chem. 274, 7334–7340
  9. Bischoff, J.R., et al. (1998). A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers. EMBO J. 17, 3052–3065
Code CY-1174
Product Type Kinase Assay Kit
Size 96 Assays
Price
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