CycLex® CaM-kinase II assay kit

CycLex CaM-kinase II Assay Kit.

Specifications:

Description

The CycLex CaM kinase II Assay Kit is primarily designed to measure the activities of purified Ca2+/Calmodulin dependent protein kinase II (CaM kinase II) for the rapid and sensitive evaluation of inhibitors or activators. It can be used for 96 assays.

Target: CaMKII
Product Type: Kinase Assay Kit
Size: 96 Assays
Research Area / Disease: Cell Biology, Neuroscience
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Components

  • Microplate
  • 10X Wash Buffer
  • Kinase Buffer
  • 50X CaCl2
  • 50X EGTA
  • 20X ATP
  • HRP conjugated Detection Antibody
  • Substrate Reagent
  • Stop Solution

Citations

  1. Reiken, Steve, et al. “Alzheimer’s‐like signaling in brains of COVID‐19 patients.” Alzheimer’s & Dementia (2022).
  2. Balakrishnan, Karthik, et al. “Targeting the interaction of GABAB receptors with CaMKII with an interfering peptide restores receptor expression after cerebral ischemia and inhibits progressive neuronal death in mouse brain cells and slices.” Brain Pathology (2022): e13099.
  3. Chen, Wei-Chia, et al. “Benzenesulfonamide derivatives as calcium/calmodulin-dependent protein kinase inhibitors and antiviral agents against dengue and zika virus infections.” Journal of Medicinal Chemistry 63.3 (2020): 1313-1327.
  4. Genders, Amanda J., et al. “Reducing hepatic PKD activity lowers circulating VLDL cholesterol.” Journal of Endocrinology 246.3 (2020): 265-276.
  5. Shao, Bin, et al. “Plasma CAMK2A predicts chemotherapy resistance in metastatic triple negative breast cancer.” International Journal of Clinical and Experimental Pathology 11.2 (2018): 650.
  6. Kumrungsee, Thanutchaporn, et al. “Identification of peptides in wheat germ hydrolysate that demonstrate calmodulin-dependent protein kinase II inhibitory activity.” Food Chemistry 213 (2016): 329-335.
  7. Wang N et al. Active calcium/calmodulin-dependent protein kinase II (CaMKII) regulates NMDA receptor mediated postischemic long-term potentiation (i-LTP) by promoting the interaction between CaMKII and NMDA receptors in ischemia. Neural Plast. 2014, 827161 (2014)
  8. Chen L et al. The role of low levels of fullerene C60 nanocrystals on enhanced learning and memory of rats through persistent CaMKII activation. Biomaterials. 35, 9269-79 (2014)
  9. Hamdani N et al. Crucial role for Ca2(+)/calmodulin-dependent protein kinase-II in regulating diastolic stress of normal and failing hearts via titin phosphorylation. Circ Res. 112, 664-74 (2013)
  10. Lu Q et al. Ca2+/calmodulin-dependent protein kinase II contributes to hypoxic ischemic cell death in neonatal hippocampal slice cultures. PLoS One. 8, e70750 (2013)
  11. Rand Shahin, Mutasem O. Taha. Elaborate Ligand-Based Modeling and Subsequent Synthetic Exploration Unveil New Nanomolar Ca2+/Calmodulin-Dependent Protein Kinase II Inhibitory Leads. Bioorganic & Medicinal Chemistry. 20, 377-400 (2011)
  12. Edward C. Stack et al. A novel role for calcium/calmodulin kinase II within the brainstem pedunculopontine tegmentum for the regulation of wakefulness and rapid eye movement sleep. Journal of Neurochemistry 112, 271-81 (2010)
  13. Karthik Bodhinathan, Ashok Kumar, and Thomas C. Foster. Intracellular Redox State Alters NMDA Receptor Response during Aging through Ca2+/Calmodulin-Dependent Protein Kinase II. J. Neurosci. 30, 1914-24 (2010)
  14. Yoo Jung Yang et al. Effects of scoparone on dopamine release in PC12 cells. Fitoterapia. 81, 497-502 (2010)

Using purified mouse tissue:

  1. Hamdani, N et al. Crucial Role for Ca2 /Calmodulin-Dependent Protein Kinase-II in Regulating Diastolic Stress of Normal and Failing Hearts via Titin Phosphorylation. Circulation Research 112.4 (2013),
  2. Wang, N et al. Active Calcium/Calmodulin-Dependent Protein Kinase II (CaMKII) Regulates NMDA Receptor Mediated Postischemic Long-Term Potentiation (i-LTP) by Promoting the Interaction between CaMKII and NMDA Receptors in Ischemia. Neural Plasticity, (2014),

References

  • Andy HUDMON1 and Howard SCHULMAN1 Biochem. J. 364, 593-611, 2002
  • Van Eldik, L., and Watterson, M. Calmodulin and Signal Transduction, Academic Press, New York, 1998
  • Erondu, N. E. and Kennedy, M. B. Regional distribution of type II Ca2+/calmodulin-dependent protein kinase in rat brain. J. Neurosci. 5, 3270-3277, 1985
  • Soderling, T. R. Biochim. Biophys. Acta 1297, 131–138, 1996
  • Kolodziej, S. J., Hudmon, A., Waxham, M. N., and Stoops, J. K. J. Biol. Chem. 275, 14354–14359, 2000
  • Kanaseki, T., Ikeuchi, Y., Sugiura, H., and Yamauchi, T. J. Cell Biol. 115, 1049-1060, 1991
  • Brocke, L., Chiang, L. W., Wagner, P. D., and Schulman, H. J. Biol. Chem. 274, 22713–22722,1999
Code CY-1173
Product Type Kinase Assay Kit
Size 96 Assays
Price
$654.60
Qty: