Products Research Area/Disease Cell Biology CycLex® CK2(Casein kinase II) Kinase Assay/Inhibitor Screening Kit

CycLex® CK2(Casein kinase II) Kinase Assay/Inhibitor Screening Kit

CycLex CK2(Casein kinase II) Kinase Assay/Inhibitor Screening Kit.

Data Sheet

Specifications:

Description

The CycLex CK2 Assay/Inhibitor Screening Kit is designed to measure the activity of purified Casein Kinase-2 (CK2) for the rapid and sensitive evaluation of CK2 inhibitors or activators. It can be used for 96 assays.

Target:	CK2
Product Type:	Kinase Assay Kit
Size:	96 Assays
Research Area / Disease:	Cell Biology
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.

Components

Microplate
10x Wash Buffer
Kinase Buffer
20x ATP
HRP conjugated Detection Antibody
Substrate Reagent
Stop Solution

Citations

Sun, Zhimin, et al. “Casein kinase 2 attenuates brain injury induced by intracerebral hemorrhage via regulation of NR2B phosphorylation.” Frontiers in Cellular Neuroscience 16 (2022)
Floris A et al. Star-related lipid transfer protein 10 (STARD10): a novel key player in alcohol-induced breast cancer progression. J Exp Clin Cancer Res. 38, 4. (2019)
Im J, Nho RS. Fibroblasts from patients with idiopathic pulmonary fibrosis are resistant to cisplatin-induced cell death via enhanced CK2-dependent XRCC1 activity. Apoptosis. 24, 499-510 (2019)
Lee HR et al. Attenuation of β-amyloid-induced tauopathy via activation of CK2α/SIRT1: targeting for cilostazol. J Neurosci Res. 92, 206-17 (2014)
Thonel A et al. Regulation of the proapoptotic functions of prostate apoptosis response-4 (Par-4) by casein kinase 2 in prostate cancer cells. Cell Death Dis. 5, e1016 (2014)
Lee YS et al. Helicobacter pylori induces cell migration and invasion through casein kinase 2 in gastric epithelial cells. Helicobacter. 19, 465-75 (2014)
Jung EB et al. Casein kinase 2 inhibition attenuates cholesterol oxidation product-induced apoptosis by suppressing the activation of the mitochondrial pathway and the caspase-8- and bid-dependent pathways. Neurochem Int. 65, 30-9 (2014)
Lee WH, Lee HH, Vo MT, Kim HJ, Ko MS, Im YC, Min YJ, Lee BJ, Cho WJ, Park JW.; Casein kinase 2 regulates the mRNA-destabilizing activity of tristetraprolin. J Biol Chem. 286, 21577-87 (2011)
Guixia Wang, Yunqian Pan, Kashif A. Ahmad and Khalil Ahmed; Protein B23/Nucleophosmin/Numatrin nuclear dynamics in Relation to Protein Kinase CK2 and Apoptotic Activity in Prostate Cells. Biochemistry, 49: 3842_3852, (2010)
Izumi Oinuma, Yuri Ito, Hironori Katoh, and Manabu Negishi; Semaphorin 4D/Plexin-B1 Stimulates PTEN Activity through R-Ras GTPase-activating Protein Activity, Inducing Growth Cone Collapse in Hippocampal Neurons. J Biol Chem., 285: 28200-28209,(2010)

References

Lozeman, F.J., Litchfield, D.W., Piening, C., Takio, K., Walsh, K.A. and Krebs, E.G. (1990) Isolation and characterization of human cDNA clones encoding the α and α´ subunits of CK2. Biochemistry 29, 8436–8447
Litchfield, D.W., Lozeman, F.J., Piening, C., Sommercorn, J., Takio, K., Walsh, K.A. and Krebs, E.G. (1990) Subunit structure of CK2 from bovine testis: demonstration that the α and α´ subunits are distinct polypeptides. J. Biol. Chem. 265, 7638–7644
Maridor, G., Park, W., Krek, W. and Nigg, E.A. (1991) CK2. cDNA sequences, developmental expression and tissue distribution of mRNAs for α, α´ and β subunits of the chicken enzyme. J. Biol. Chem. 266, 2362–2368
Munstermann, U., Fritz, G., Seitz, G., Lu, Y.P., Schneider, H.R. and Issinger, O.-G. (1990) CK2 is elevated in solid human tumors and rapidly proliferating non-neoplastic tissue. Eur. J. Biochem. 189, 251–257
Pepperkok, R., Lorenz, P., Ansorge, W. and Pyerin, W. (1994) CK2 is required for transition of G0/G1, early G1, and G1/S phases of the cell cycle. J. Biol. Chem. 269, 6986–6991
Landesman-Bollag, E., Romieu-Mourez, R., Song, D.H., Sonenshein, G.E., Cardiff, R.D. and Seldin, D.C. (2001) Protein kinase CK2 in mammary gland tumorigenesis. Oncogene 20, 3247–3257
Seldin, D.C. and Leder, P. (1995) CK2 alpha transgene-induce murine lymphoma: relation to theileriosis in cattle. Science 267, 894–897
Landesman-Bollag, E., Channavajhala, P.L., Cardiff, R.D. and Seldin, D.C. (1998) p53 deficiency and mis-expression of protein kinase CK2a collaborate in the development of thymic lymphomas in mice. Oncogene 16, 2965–2974
Channavajhala, P. and Seldin, D.C. (2002) Functional interaction of protein kinase CK2 and c-Myc in lymphomagenesis. Oncogene 21, 5280–5288
Sayed, M., Pelech, S., Wong, C., Marotta, A. and Salh, B. (2001) Protein kinase CK2 is involved in G2 arrest and apoptosis following spindle damage in epithelial cells. Oncogene 20, 6994–7005
Desagher, S., Osen-Sand, A., Montessuit, S., Magnenat, E., Vilbois, F., Hochmann, A., Journot, L., Antonsson, B. and Martinou, J.C. (2001) Phosphorylation of bid by casein kinases I and II regulates its cleavage by caspase 8. Mol. Cell 8, 601–611
Li, P., Li, J., Muller, E., Otto, A., Dietz, R. and von Harsdorf, R. (2002) Phosphorylation by protein kinase CK2. A signaling switch for the caspase-inhibiting protein ARC. Mol. Cell 10, 247–258
Wang, H., Davis, A., Yu, S. and Ahmed, K. (2001) Response of cancer cells to molecular interruption of the CK2 signal. Mol. Cell. Biochem. 227, 167–174