CycLex® AKT/PKB Kinase Assay/Inhibitor Screening Kit

CycLex AKT/PKB Kinase Assay/Inhibitor Screening Kit.

Specifications:

Description

The CycLex AKT/PKB kinase Assay/Inhibitor Screening Kit is designed to measure the activities of purified AKTs for the rapid and sensitive evaluation of inhibitors or activators It can be used for 96 assays.

Target: Akt1
Product Type: Kinase Assay Kit
Size: 96 Assays
Research Area / Disease: Cell Biology
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Components

  • Microplate
  • 10x Wash Buffer
  • Kinase Buffer
  • 20x ATP
  • HRP-conjugated Detection Antibody
  • Substrate Reagent
  • Stop Solution

Citations

  1. Bresin A et al. T Cell Leukemia/Lymphoma 1A is essential for mouse epidermal keratinocytes proliferation promoted by insulin-like growth factor 1. PLoS One. 13, e0204775 (2018)
  2. Hideo Mure, Kazuhito Matsuzaki, Keiko T. Kitazato, Yoshifumi Mizobuchi, Kazuyuki Kuwayama, Teruyoshi Kageji, and Shinji Nagahiro; Akt2 and Akt3 play a pivotal role in malignant gliomas. Neuro Oncology, 12: 221-232, 2010

References

  1. Staal, S. P. Proc. Nat. Acad. Sci. 84, 5034-5037, 1987
  2. Andjelkovic M, Alessi DR, Meier R, Fernandez A, Lamb NJ, Frech M, Cron P, Cohen P, Lucocq JM, Hemmings BA. J Biol Chem. 272, 31515-24, 1997
  3. Meier, R., Alessi, D. R., Cron, P., Andjelkovic, M. and Hemmings, B. A. J. Biol. Chem. 272, 30491–30497, 1997
  4. Cross, D. A.E., Alessi, D. R., Cohen, P., Andjelkovi , M., and Hemmings, B. A. Nature 378, 785-789, 1995
  5. Kohn, A. D., Summers, S. A., Birnbaum, M. J., and Roth, R. A. J. Biol. Chem. 271, 31372-31378, 1996
  6. Dudek, H., Datta, S. R., Franke, T. F., Birnbaum, M. J., Yao, R., Cooper, G. M., Segal, R. A., Kaplan, D. R., and Greenberg, M. E. Science 275, 661-665, 1997
  7. Cheng, J.Q., Godwin, A. K., Bellacosa, A., Taguchi, T., Franke, T. F., Hamilton, T. C., Tsichlis, P. N., and Testa, J. R. Proc. Nat. Acad. Sci. 89, 9267-9271, 1992
  8. Bellacosa, A.et al. Int. J. Cancer 64, 280-285, 1995
  9. Cheng, J.Q., Ruggeri, B., Klein, W. M., Sonoda, G., Altomare, D. A., Watson, D. K., and Testa, J. R. Proc. Nat. Acad. Sci. 93, 3636-3641, 1996
  10. Toshiyuki Obata, Michael B. Yaffe, German G. Leparc, Elizabeth T. Piro, Hiroshi Maegawa, Atsunori Kashiwagi, Ryuichi Kikkawa, and Lewis C. J. Biol. Chem. 275: 36108 – 36115, 2000.
  11. Vanhaesebroeck, B.; Alessi, D. R. Biochem. J. 346, 561-576, 2000. (Review)
  12. Franke, T. F., Kaplan, D. R., and Cantley, L. C. Cell 88, 435-437, 1997 (Review)
  13. Hemmings, B. A. Science 275, 628-630, 1997 (Review)
Code CY-1168
Product Type Kinase Assay Kit
Size 96 Assays
Price
$654.60
Qty: