Products Research Area/Disease Cell Biology CycLex® AKT/PKB Kinase Assay/Inhibitor Screening Kit

CycLex® AKT/PKB Kinase Assay/Inhibitor Screening Kit

CycLex AKT/PKB Kinase Assay/Inhibitor Screening Kit.

Specifications:

Description

The CycLex AKT/PKB kinase Assay/Inhibitor Screening Kit is designed to measure the activities of purified AKTs for the rapid and sensitive evaluation of inhibitors or activators It can be used for 96 assays.

Target:	Akt1
Product Type:	Kinase Assay Kit
Size:	96 Assays
Research Area / Disease:	Cell Biology
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.

Components

Microplate
10x Wash Buffer
Kinase Buffer
20x ATP
HRP-conjugated Detection Antibody
Substrate Reagent
Stop Solution

Citations

Bresin A et al. T Cell Leukemia/Lymphoma 1A is essential for mouse epidermal keratinocytes proliferation promoted by insulin-like growth factor 1. PLoS One. 13, e0204775 (2018)
Hideo Mure, Kazuhito Matsuzaki, Keiko T. Kitazato, Yoshifumi Mizobuchi, Kazuyuki Kuwayama, Teruyoshi Kageji, and Shinji Nagahiro; Akt2 and Akt3 play a pivotal role in malignant gliomas. Neuro Oncology, 12: 221-232, 2010

References

Staal, S. P. Proc. Nat. Acad. Sci. 84, 5034-5037, 1987
Andjelkovic M, Alessi DR, Meier R, Fernandez A, Lamb NJ, Frech M, Cron P, Cohen P, Lucocq JM, Hemmings BA. J Biol Chem. 272, 31515-24, 1997
Meier, R., Alessi, D. R., Cron, P., Andjelkovic, M. and Hemmings, B. A. J. Biol. Chem. 272, 30491–30497, 1997
Cross, D. A.E., Alessi, D. R., Cohen, P., Andjelkovi , M., and Hemmings, B. A. Nature 378, 785-789, 1995
Kohn, A. D., Summers, S. A., Birnbaum, M. J., and Roth, R. A. J. Biol. Chem. 271, 31372-31378, 1996
Dudek, H., Datta, S. R., Franke, T. F., Birnbaum, M. J., Yao, R., Cooper, G. M., Segal, R. A., Kaplan, D. R., and Greenberg, M. E. Science 275, 661-665, 1997
Cheng, J.Q., Godwin, A. K., Bellacosa, A., Taguchi, T., Franke, T. F., Hamilton, T. C., Tsichlis, P. N., and Testa, J. R. Proc. Nat. Acad. Sci. 89, 9267-9271, 1992
Bellacosa, A.et al. Int. J. Cancer 64, 280-285, 1995
Cheng, J.Q., Ruggeri, B., Klein, W. M., Sonoda, G., Altomare, D. A., Watson, D. K., and Testa, J. R. Proc. Nat. Acad. Sci. 93, 3636-3641, 1996
Toshiyuki Obata, Michael B. Yaffe, German G. Leparc, Elizabeth T. Piro, Hiroshi Maegawa, Atsunori Kashiwagi, Ryuichi Kikkawa, and Lewis C. J. Biol. Chem. 275: 36108 – 36115, 2000.
Vanhaesebroeck, B.; Alessi, D. R. Biochem. J. 346, 561-576, 2000. (Review)
Franke, T. F., Kaplan, D. R., and Cantley, L. C. Cell 88, 435-437, 1997 (Review)
Hemmings, B. A. Science 275, 628-630, 1997 (Review)