Products Research Area/Disease Cell Biology CycLex® Aurora-A kinase Assay/Inhibitor Screening Kit

CycLex® Aurora-A kinase Assay/Inhibitor Screening Kit

CycLex Aurora-A kinase Assay/Inhibitor Screening Kit.

Data Sheet

Specifications:

Description

The Aurora-A kinase Assay/Inhibitor Screening Kit is designed to measure the activity of purified Aurora-A and for the rapid and sensitive evaluation of inhibitors or activators of Aurora-A. It can be used for 96 assays.

Target:	Aurora-A
Product Type:	Kinase Assay Kit
Size:	96 Assays
Research Area / Disease:	Cell Biology
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.

Components

Microplate (coated with recombinant Lats2-N-ter as an Aurora-A substrate)
0X Wash Buffer
Kinase Buffer, 20X ATP
Anti-Phospho-Lats2-S83 Monoclonal Antibody (ST-3B11)
HRP-conjugated Anti-mouse IgG
Substrate Reagent
Stop Solution

Citations

Mergen M et al. The nephronophthisis gene product NPHP2/Inversin interacts with Aurora A and interferes with HDAC6-mediated cilia disassembly. Nephrol Dial Transplant. 28, 2744-53 (2013)

References

Zhou, H., Kuang, J., Zhong, L., Kuo, W. L., Gray, J. W., Sahin, A., Brinkley, B. R., and Sen, S. Nat. Genet. 20, 189-193, 1998
Bischoff, J. R., Anderson, L., Zhu, Y., Mossie, K., Ng, L., Souza, B., Schryver, B., Flanagan, P.,
Clairvoyant, F., Ginther, C., Chan, C. S., Novotny, M., Slamon, D. J., and Plowman, G. D. EMBO J. 17, 3052-3065, 1998
Glover, D. M., Leibowitz, M. H., McLean, D. A., and Parry, H. Cell 81, 95-105, 1995
Chan, C. S., and Botstein, D. Genetics 135, 677-691, 1993
Glover, D..Mol.Cell. 12(4):797-9, 2003