Products Research Area/Disease Cell Biology CycLex® Polo-like kinase 1 Assay/Inhibitor Screening Kit

CycLex® Polo-like kinase 1 Assay/Inhibitor Screening Kit

CycLex Polo-like kinase 1 Assay/Inhibitor Screening Kit.

Specifications:

Description

The Polo-like kinase 1 Assay/Inhibitor Screening Kit is designed to measure the activity of purified polo-like kinase 1 (Plk1) and for the rapid and sensitive evaluation of inhibitors using recombinant Plk1. It can be used for 96 assays.

Target:	Plk1
Product Type:	Kinase Assay Kit
Size:	96 Assays
Research Area / Disease:	Cell Biology
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.

Components

Microplate
10X Wash Buffer
Kinase Buffer
20X ATP
Anti-Phospho-Threonine Polyclonal Antibody
HRP conjugated Anti-rabbit IgG
Substrate Reagent
Stop Solution

Citations

Hyo-Eun Yoon, Soo-A Kim, Hong Seok Choi, Mee-Young Ahn, Jung-Hoon Yoon, Sang-Gun Ahn; Inhibition of Plk1 and Pin1 by 5’-nitro-indirubinoxime suppresses human lung cancer cells. Cancer Letters. 316: 97-104. 2012,
Kim SM et al. Enhanced cellular uptake of a TAT-conjugated peptide inhibitor targeting the polo-box domain of polo-like kinase 1. Amino Acids. 46, 2595-603 (2014),
Srinivasrao G et al. Design and synthesis of a cell-permeable, drug-like small molecule inhibitor targeting the polo-box domain of polo-like kinase 1. PLoS One. 9, e107432 (2014)

References

Lane, H. A. & Nigg, E. A. Trends Cell Biol. 7, 63-68, 1997
Glover, D. M., Hagan, I. M. & Tavares, A. A. M. Genes Dev. 12, 3777-3787, 1998
Sunkel, C. E. & Glover, D. M. J. Cell Sci. 89, 25-38, 1988
Ohkura, H., Hagan, I. & Glover, D. M. Genes Dev. 9, 1059-1073, 1995
Song, S. & Lee, K. S. J. Cell Biol. 152, 451-469, 2001
Lane, H. A. & Nigg, E. A. J. Cell Biol. 135, 1701-1713, 1996
Kotani, S., Tugendreich, S., Fujii, M., Jorgensen, P., Watanabe, N., Hoog, C., Hieter, P. & Todokoro, K. Mol. Cell 1, 371-380, 1998
Alexandru, G., Uhlmann, F., Mechtler, K., Poupart, M. & Nasmyth, K. Cell 105, 459-472, 2001
Liu, X.; Erikson, R. L: Polo-like kinase (Plk1) depletion induces apoptosis in cancer cells. Proc. Nat. Acad. Sci. 100, 5789-5794, 2003