CycLex® Polo-like kinase 1 Assay/Inhibitor Screening Kit

CycLex Polo-like kinase 1 Assay/Inhibitor Screening Kit.

Specifications:

Description

The Polo-like kinase 1 Assay/Inhibitor Screening Kit is designed to measure the activity of purified polo-like kinase 1 (Plk1) and for the rapid and sensitive evaluation of inhibitors using recombinant Plk1. It can be used for 96 assays.

Target: Plk1
Product Type: Kinase Assay Kit
Size: 96 Assays
Research Area / Disease: Cell Biology
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Components

  • Microplate
  • 10X Wash Buffer
  • Kinase Buffer
  • 20X ATP
  • Anti-Phospho-Threonine Polyclonal Antibody
  • HRP conjugated Anti-rabbit IgG
  • Substrate Reagent
  • Stop Solution

Citations

  1. Hyo-Eun Yoon, Soo-A Kim, Hong Seok Choi, Mee-Young Ahn, Jung-Hoon Yoon, Sang-Gun Ahn; Inhibition of Plk1 and Pin1 by 5’-nitro-indirubinoxime suppresses human lung cancer cells. Cancer Letters. 316: 97-104. 2012,
  2. Kim SM et al. Enhanced cellular uptake of a TAT-conjugated peptide inhibitor targeting the polo-box domain of polo-like kinase 1. Amino Acids. 46, 2595-603 (2014),
  3. Srinivasrao G et al. Design and synthesis of a cell-permeable, drug-like small molecule inhibitor targeting the polo-box domain of polo-like kinase 1. PLoS One. 9, e107432 (2014)

References

  1. Lane, H. A. & Nigg, E. A. Trends Cell Biol. 7, 63-68, 1997
  2. Glover, D. M., Hagan, I. M. & Tavares, A. A. M. Genes Dev. 12, 3777-3787, 1998
  3. Sunkel, C. E. & Glover, D. M. J. Cell Sci. 89, 25-38, 1988
  4. Ohkura, H., Hagan, I. & Glover, D. M. Genes Dev. 9, 1059-1073, 1995
  5. Song, S. & Lee, K. S. J. Cell Biol. 152, 451-469, 2001
  6. Lane, H. A. & Nigg, E. A. J. Cell Biol. 135, 1701-1713, 1996
  7. Kotani, S., Tugendreich, S., Fujii, M., Jorgensen, P., Watanabe, N., Hoog, C., Hieter, P. & Todokoro, K. Mol. Cell 1, 371-380, 1998
  8. Alexandru, G., Uhlmann, F., Mechtler, K., Poupart, M. & Nasmyth, K. Cell 105, 459-472, 2001
  9. Liu, X.; Erikson, R. L: Polo-like kinase (Plk1) depletion induces apoptosis in cancer cells. Proc. Nat. Acad. Sci. 100, 5789-5794, 2003
Code CY-1163
Product Type Kinase Assay Kit
Size 96 Assays
Price
$654.60
Qty: