CycLex® Cyclic GMP dependent protein kinase (cGK) Assay Kit

  • Code # CY-1161
  • Size 96 Assays
  • Price
    $595.00
Specifications

Background

Nitric oxide (NO) and a broad spectrum of hormones, drugs, and toxins raise intracellular cGMP concentrations and thereby regulate a great variety of functions, including smooth muscle relaxation, neuronal excitability, and epithelial electrolyte transport. Pfeifer et al. (1996) noted that depending on the tissue, an increase in cGMP concentration leads to the activation of different receptors, such as cyclic nucleotide phosphodiesterases. The identification of the physiologic mediator of cGMP is complicated by the existence of 2 forms of cGMP-dependent protein kinase (cGK), types I and II, which are encoded by distinct genes. Smooth muscle, platelets, and cerebellum contain high concentrations of cGK-I, whereas cGK-II is highly concentrated in brain, lung, and intestinal mucosa. The function of cGK-II is not well understood, although there is evidence that it mediates intestinal secretion of water and electrolytes induced by the E. coli toxin STa and the intestinal peptide guanylin. Activation of cyclic GMP-dependent protein kinase (cGK) is an important event in the regulation of blood pressure and platelet function. Upstream signals include the generation of nitric oxide (NO) by NO synthases and the subsequent rise in cGMP levels mediated by NO-dependent guanyl cyclases (GCs). The identification of new cGK activators by high throughput screening (HTS) may lead to the development of a novel class of therapeutics for the treatment of cardiovascular diseases.
  • Components:
    • Microplate (coated with recombinant G-kinase substrate)
    • 10~Wash Buffer
    • Kinase Buffer, 20~ATP
    • HRP conjugated Detection Antibody
    • Substrate Reagent
    • Stop Solution
  • Description:

    The CycLex Research Product CycLexCyclic GMP dependent protein kinase (cGK) Assay Kit is primarily designed to measure the activities of purified the cGK family of kinases for the rapid and sensitive evaluation of inhibitors or activators. It can be used for 96 assays.

  • Product Type:
    Kinase Assay Kit
  • Research Area:
    Cell Biology
  • Short Description:

    CycLex Cyclic GMP dependent protein kinase (cGK) Assay Kit.

  • Size:
    96 Assays
Citations
  1. Aggarwal S et al. Nitration of tyrosine 247 inhibits protein kinase G-1α activity by attenuating cyclic guanosine monophosphate binding. J Biol Chem. 289, 7948-61 (2014),
  2. Aggarwal S, Gross CM, Kumar S, Datar S, Oishi P, Kalkan G, Schreiber C, Fratz S, Fineman JR, Black SM.; Attenuated vasodilatation in lambs with endogenous and exogenous activation of cGMP signaling: role of protein kinase G nitration. J Cell Physiol. 226: 3104-13. 2011,
  3. Andersen A et al. Right ventricular hypertrophy and failure abolish cardioprotection by ischaemic pre-conditioning. Eur J Heart Fail. 15, 1208-14 (2013),
  4. Bahar Hesabi, Robert S. Danziger, Kumar U. Kotlo; Heterogeneous nuclear ribonucleoprotein A1 is a novel cellular target of atrial natriuretic peptide signaling in renal epithelial cells. Cell Signal. 24: 1100-8. 2012,
  5. Bivalacqua TJ et al. Sildenafil citrate-restored eNOS and PDE5 regulation in sickle cell mouse penis prevents priapism via control of oxidative/nitrosative stress. PLoS One. 8, e68028 (2013),
  6. Chan S, Chan J, Hsu K, Li F, Sun E, Chen W, Chang A.; Amelioration of central cardiovascular regulatory dysfunction by tropomyocin receptor kinase B in mevinphos intoxication model of brain stem death. Br J Pharmacol.164: 2015-28. 2011,
  7. Chau VQ, Salloum FN, Hoke NN, Abbate A, Kukreja RC.; Mitigation of the Progression of Heart Failure with Sildenafil Involves Inhibition of RhoA/Rho-Kinase Pathway. Am J Physiol Heart Circ Physiol. 300, H2272-H2279. 2011,
  8. da Silva Lima V et al. Uroguanylin inhibits H-ATPase activity and surface expression in renal distal tubules by a PKG-dependent pathway. Am J Physiol Cell Physiol. 307, C532-41 (2014),
  9. Gebska MA, Stevenson BK, Hemnes AR, Bivalacqua TJ, Haile A, Hesketh GG, Murray CI, Zaiman AL, Halushka MK, Krongkaew N, Strong TD, Cooke CA, El-Haddad H, Tuder RM, Berkowitz DE, Champion HC.; Phosphodiesterase-5A (PDE5A) is localized to the endothelial caveolae and modulates NOS3 activity. Cardiovasc Res. 90: 353-63, 2011,
  10. Haider HKh, Lee YJ, Jiang S, Ahmed RP, Ryon M, Ashraf M.; Phosphodiestrase inhibition with tadalafil provides longer and sustained protection of stem cells. Am J Physiol Heart Circ Physiol, 299: H1395-404, 2010,
  11. Hajime Nagasu, Minoru Satoh, Kengo Kidokoro, Yuko Nishi, Keith M Channon, Tamaki Sasaki, and Naoki Kashihara; Endothelial dysfunction promotes the transition from compensatory renal hypertrophy to kidney injury after unilateral nephrectomy in mice. Am J Physiol Renal Physiol. 302: F1402-8. 2012,
  12. Harm J Bogaard, Ramesh Natarajan, Shiro Mizuno, Antonio Abbate, Philip J Chang, Vinh Q Chau, Nicholas N Hoke, Donatas Kraskauskas, Michael Kasper, Fadi N Salloum, and Norbert F Voelkel; Adrenergic Receptor Blockade Reverses Right Heart Remodeling and Dysfunction in Pulmonary Hypertensive Rats. Am J Respir Crit Care Med, 182: 652-60, 2010,
  13. Hoke NN, Salloum FN, Kass DA, Das A, Kukreja RC.; Preconditioning by phosphodiesterase-5 inhibition improves therapeutic efficacy of adipose-derived stem cells following myocardial infarction in mice. Stem Cells. 30: 326-35. 2012,
  14. Hong-Guang Nie, Wei Zhang, Dong-Yun Han, Qing-Nan Li, Jun Li, Run-Zhen Zhao, Xue-Feng Su, Ji-Bin Peng, and Hong-Long Ji; 8-pCPT-cGMP stimulates alpha beta gamma ENaC activity in oocytes as an external ligand requiring specific nucleotide moieties. Am J Physiol Renal Physiol, 298: F323 - F334, 2010,
  15. Hu LF, Li Y, Neo KL, Yong QC, Lee SW, Tan BK, Bian JS.; Hydrogen sulfide regulates Na+/H+ exchanger activity via stimulation of Phosphoinositide 3-kinase/Akt and protein kinase G pathways. J Pharmacol Exp Ther. 339: 726-35. 2011,
  16. Jaba IM et al. NO triggers RGS4 degradation to coordinate angiogenesis and cardiomyocyte growth. J Clin Invest. 123, 1718-31 (2013),
  17. Jin Z et al. Beneficial effects of tadalafil on left ventricular dysfunction in doxorubicin-induced cardiomyopathy. J Cardiol. 62, 110-6 (2013),
  18. Karakhanova S et al. Interlude of cGMP and cGMP/protein kinase G type 1 in pancreatic adenocarcinoma cells. Pancreas. 43, 784-94 (2014),
  19. Keating JA et al. Mosquito protein kinase G phosphorylates flavivirus NS5 and alters flight behavior in Aedes aegypti and Anopheles gambiae. Vector Borne Zoonotic Dis. 13, 590-600 (2013),
  20. Keating JA et al. West Nile virus methyltransferase domain interacts with protein kinase G. Virol J. 10, 242 (2013),
  21. Larocca TJ, Schwarzkopf M, Altman P, Zhang S, Gupta A, Gomes I, Alvin Z, Champion HC, Haddad G, Hajjar RJ, Devi LA, Schecter AD, Tarzami ST.; beta2-Adrenergic Receptor Signaling in the Cardiac Myocyte is Modulated by Interactions with CXCR4. J Cardiovasc Pharmacol,56: 548-59, 2010,
  22. Ranek MJ et al. Protein kinase g positively regulates proteasome-mediated degradation of misfolded proteins. Circulation. 128, 365-76 (2013),
  23. Sagredo A et al. Ovariectomy increases the participation of hyperpolarizing mechanisms in the relaxation of rat aorta. PLoS One. 8, e73474 (2013),
  24. Saisudha Koka, Anindita Das, Shu-Guang Zhu, David Durrant, Lei Xi, and Rakesh C. Kukreja; Long-Acting Phosphodiesterase-5 Inhibitor Tadalafil Attenuates Doxorubicin-Induced Cardiomyopathy without Interfering with Chemotherapeutic Effect. J Pharmacol Exp Ther, 334: 1023-1030, 2010,
  25. Salloum FN, Das A, Samidurai A, Hoke NN, Chau VQ, Ockaili RA, Stasch JP, Kukreja RC.; Cinaciguat - a novel activator of soluble guanylate cyclase, protects against ischemia/reperfusion injury: Role of hydrogen sulfide. Am J Physiol Heart Circ Physiol. 302: H1347-54. 2012,
  26. Sasaki H et al. PDE5 inhibitor efficacy is estrogen dependent in female heart disease. J Clin Invest. 124, 2464-71 (2014),
  27. Saurabh Aggarwal, Ruslan Rafikov, Christine M. Gross, Sanjiv Kumar, Daniel Pardo and Stephen M. Black; Purification and functional analysis of protein kinase G-1α using a bacterial expression system. Protein Expression and Purification 79, 271-276, 2011,
  28. Seya K et al. Cytosolic Ca2+-induced apoptosis in rat cardiomyocytes via mitochondrial NO-cGMP-protein kinase G pathway. J Pharmacol Exp Ther. 344, 77-84 (2013),
  29. Tarès S et al. Environment exploration and colonization behavior of the pea aphid associated with the expression of the foraging gene. PLoS One. 8, e65104 (2013),
  30. Tassone EJ et al. Angiotensin (1-7) counteracts the negative effect of angiotensin II on insulin signalling in HUVECs. Cardiovasc Res. 99, 129-36 (2013),
  31. Wang L et al. Protein kinase G1 α overexpression increases stem cell survival and cardiac function after myocardial infarction. PLoS One. 8, e60087 (2013),
  32. Xu J et al. cGMP accumulation causes photoreceptor degeneration in CNG channel deficiency: evidence of cGMP cytotoxicity independently of enhanced CNG channel function. J Neurosci. 33, 14939-48 (2013),
  33. Zhou K et al. 17β-estradiol induces vasorelaxation by stimulating endothelial hydrogen sulfide release. Mol Hum Reprod. 19, 169-76 (2013)
References
  1. Pfeifer, A., Aszodi, A., Seidler, U., Ruth, P., Hofmann, F. and Fassler, R.: Intestinal secretory defects and dwarfism in mice lacking cGMP-dependent protein kinase II. Science 274: 2082-2084, 1996
  2. Tamura, N., Itoh, H., Ogawa, Y., Nakagawa, O., Harada, M., Chun, T.-H., Suga, S., Yoshimasa, T. and Nakao, K.: cDNA cloning and gene expression of human type I-alpha cGMP-dependent protein kinase. Hypertension 27: 552-557, 1996
  3. Orstavik, S., Natarajan, V., Tasken, K., Jahnsen, T. and Sandberg, M.: Characterization of the human gene encoding the type I-alpha and type I-beta cGMP-dependent protein kinase (PRKG1). Genomics 42: 311-318, 1997
  4. Li, Z., Xi, X., Gu, M., Feil, R., Ye, R. D., Eigenthaler, M., Hofmann, F. and Du, X.: A stimulatory role for cGMP-dependent protein kinase in platelet activation. Cell 112: 77-86, 2003
  5. Yamahara K, Itoh H, Chun TH, Ogawa Y, Yamashita J, Sawada N, Fukunaga Y, Sone M, Yurugi-Kobayashi T, Miyashita K, Tsujimoto H, Kook H, Feil R, Garbers DL, Hofmann F, Nakao K.: Significance and therapeutic potential of the natriuretic peptides/cGMP/cGMP-dependent protein kinase pathway in vascular regeneration. Proc. Natl. Acad. Sci. U.S.A. 100(6):3404-9, 2003