CycLex® Rho-kinase Assay Kit

CycLex Rho-kinase Assay Kit.



The CycLex Research Product CycLex Rho-kinase Assay Kit is primarily designed to measure the activities of purified Rho-kinase or DMPK for the rapid and sensitive evaluation of inhibitors or activators. It can be used for 96 assays.

Target: RHO
Product Type: Kinase Assay Kit
Size: 96 Assays
Research Area / Disease: Cell Biology
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.


  • Microplate (coated with recombinant MBS C-terminus(758-1032 a.a.) as Rho-kinase substrate)
  • 10~Wash Buffer
  • Kinase Buffer
  • 20~ATP
  • HRP conjugated Detection Antibody
  • Substrate Reagent
  • Stop Solution





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  2. Bei Y et al. Activation of RhoA/Rho-kinase pathway accounts for pulmonary endothelial dysfunction in patients with chronic obstructive pulmonary disease. Physiol Rep. 1, e00105 (2013),
  3. Bourguignon LY et al. Selective matrix (hyaluronan) interaction with CD44 and RhoGTPase signaling promotes keratinocyte functions and overcomes age-related epidermal dysfunction. J Dermatol Sci. 72, 32-44 (2013),
  4. Carlos Torre, Steven J. Wang, Weiliang Xia, and Lilly Y. W. Bourguignon; Reduction of Hyaluronan-CD44_Mediated Growth, Migration, and Cisplatin Resistance in Head and Neck Cancer Due to Inhibition of Rho Kinase and PI-3 Kinase Signaling. Arch Otolaryngol Head Neck Surg, 136: 493-501, 2010,
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  6. Cyrill G_raud, Kai Schledzewski, Alexandra Demory, Diana Klein, Miriam Kaus, Francis Peyre, Carsten Sticht, Konstantin Evdokimov, Shun Lu, Astrid Schmieder, Sergij Goerdt; Liver sinusoidal endothelium: A microenvironment-dependent differentiation program in rat including the novel junctional protein liver endothelial differentiation-associated protein-1. Hepatology 52: 313-326, 2010,
  7. Duong-Quy S, Dao P, Hua-Huy T, Guilluy C, Pacaud P, Dinh-Xuan AT.; Increased Rho-kinase expression and activity and pulmonary endothelial dysfunction in smokers with normal lung function. Eur Respir J, 37: 349-55, 2011,
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  23. Naraoka M et al. Suppression of the Rho/Rho-kinase pathway and prevention of cerebral vasospasm by combination treatment with statin and fasudil after subarachnoid hemorrhage in rabbit. Transl Stroke Res. 4, 368-74 (2013),
  24. Pinzón-Daza ML et al. The cross-talk between canonical and non-canonical Wnt-dependent pathways regulates P-glycoprotein expression in human blood-brain barrier cells. J Cereb Blood Flow Metab. 34, 1258-69 (2014),
  25. Riganti C et al. Zoledronic acid restores doxorubicin chemosensitivity and immunogenic cell death in multidrug-resistant human cancer cells. PLoS One. 8, e60975 (2013),
  26. Riganti C, Pinto H, Bolli E, Belisario DC, Calogero RA, Bosia A, Cavallo F.; Atorvastatin modulates anti-proliferative and pro-proliferative signals in Her2/neu-positive mammary cancer. Biochem Pharmacol. 82: 1079-89, 2011,
  27. S. Duong-Quy, P. Dao, T. Hua-Huy, C. Guilluy, P. Pacaud and A.T. Dinh-Xuan; Increased Rho-kinases expression and activity and pulmonary endothelial dysfunction in smokers with normal lung function. Eur Respir J, 37: 349-55, 2011,
  28. Sari AN et al. Contribution of RhoA/Rho-kinase/MEK1/ERK1/2/iNOS pathway to ischemia/reperfusion-induced oxidative/nitrosative stress and inflammation leading to distant and target organ injury in rats. Eur J Pharmacol. 723, 234-45 (2014),
  29. Shahin R, Alqtaishat S, Taha MO.; Elaborate ligand-based modeling reveal new submicromolar Rho kinase inhibitors. J Comput Aided Mol Des. 26: 249-66. 2012,
  30. Sijin Liu, Shitao Li, Yuguo Du; Polychlorinated Biphenyls (PCBs) Enhance Metastatic Properties of Breast Cancer Cells by Activating Rho-Associated Kinase (ROCK). PLoS ONE 5: e11272, 2010,
  31. Takata M et al. Fasudil, a rho kinase inhibitor, limits motor neuron loss in experimental models of amyotrophic lateral sclerosis. Br J Pharmacol. 170, 341-51 (2013),
  32. Tamia K. Lapointe, Pamela M. O’Connor, Nicola L. Jones, Daniel Menard, Andre G. Buret; Interleukin-1 receptor phosphorylation activates Rho kinase to disrupt human gastric tight junctional claudin-4 during Helicobacter pylori infection. Cellular Microbiology 12: 692-703, 2010,
  33. Trevor Luke, Julie Maylor, Clark Undem, J. T. Sylvester, and Larissa A. Shimoda; Kinase dependent activation of voltage-gated Ca2+ channels by ET-1 in pulmonary arterial myocytes during chronic hypoxia. Am J Physiol Lung Cell Mol Physiol. 302: L1128-39. 2012,
  34. Trinity J. Bivalacqua, Ashley E. Ross, Travis D. Strong, Milena A. Gebska, Biljana Musicki, Hunter C. Champion and Arthur L. Burnett; Attenuated RhoA/Rho-kinase Signaling in Penis of Transgenic Sickle Cell Mice. Urology 76: 510.e7-510.e12, 2010,
  35. Tzu-Ching Chang, Yen-Chung Chen, Ming-Hua Yang, Chien-Hung Chen, En-Wei Hsing, Bor-Sheng Ko, Jun-Yang Liou,1 and Kenneth K. Wu1; Rho Kinases Regulate the Renewal and Neural Differentiation of Embryonic Stem Cells in a Cell Plating Density_Dependent Manner. PLoS One, 5: e9187, 2010,
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  37. Xiaoxiao Gu1 and Kristyn S Masters; Role of the Rho pathway in regulating valvular interstitial cell phenotype and nodule formation. AJP Heart and Circulatory Physiology, 300: H448-58, 2011,
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Code CY-1160
Product Type Kinase Assay Kit
Size 96 Assays