CycLex® HDAC8 Deacetylase Fluorometric Assay Kit Ver.2

This kit is designed for the rapid and sensitive evaluation of HDAC inhibitors using recombinant HDAC8.

Specifications:

Description

The CycLex Research Product CycLex HDAC8 Deacetylase Fluorometric Assay Kit detects HDAC activity in lysates. Primarily, the CycLex Research Product CycLex HDAC8 Deacetylase Fluorometric Assay Kit is designed for the rapid and sensitive evaluation of HDAC inhibitors using recombinant HDAC8. Additionally, any cultured primary cell, cell line, or tissue homogenate can be assayed for HDAC8 activity with the CycLex Research Product CycLex HDAC8 Deacetylase Fluorometric Assay Kit after immunoprecipitation with an appropriate HDAC8 specific antibody.

Target: HDAC8
Product Type: Kit
Size: 100 Assays
Research Area / Disease: Cancer
Storage Temperature: • Upon receipt store #5. Developer and #6. Recombinant HDAC8 at -70°C and all other components below -20°C. • Do not expose reagents to excessive light
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Components

  • HDAC Assay Buffer
  • Fluoro-Substrate Peptide (0.2 mM)
  • Fluoro-Deacetylated Peptide (0.2 mM)
  • Trichostatin A (200 μM)
  • Developer
  • Recombinant HDAC8
  • Stop Solution

References

  1. Thiagalingam, S., K. H. Cheng, H. J. Lee, N. Mineva, A. Thiagalingam, and J. F. Ponte. 2003. Histone deacetylases: unique players in shaping the epigenetic histone code. Ann. N. Y. Acad. Sci. 983:84-100
  2. Yang, X. J., and E. Seto. 2003. Collaborative spirit of histone deacetylases in regulating chromatin structure and gene expression. Curr. Opin. Genet. Dev. 13:143-153
  3. Ogryzko, V. V., T. H. Hirai, V. R. Russanova, D. A. Barbie, and B. H. Howard. 1996. Human fibroblast commitment to a senescence-like state in response to histone deacetylase inhibitors is cell cycle dependent. Mol. Cell. Biol. 16:5210-5218.
  4. Wharton, W., J. Savell, W. D. Cress, E. Seto, and W. J. Pledger. 2000. Inhibition of mitogenesis in Balb/c-3T3 cells by trichostatin A. Multiple alterations in the induction and activation of cyclin-cyclin-dependent kinase complexes. J. Biol. Chem. 275:33981-33987
  5. Bartl, S., J. Taplick, G. Lagger, H. Khier, K. Kuchler, and C. Seiser. 1997. Identification of mouse histone deacetylase 1 as a growth factor-inducible gene. Mol. Cell. Biol. 17:5033-5043
  6. He, L. Z., F. Guidez, C. Tribioli, D. Peruzzi, M. Ruthardt, A. Zelent, and P. P. Pandolfi. 1998. Distinct interactions of PML-RAR{alpha} and PLZF-RAR{alpha} with co-repressors determine differential responses to RA in APL. Nat. Genet. 18:126-135
  7. Johnstone, R. W. 2002. Histone-deacetylase inhibitors: novel drugs for the treatment of cancer. Nat. Rev. Drug Discov. 1:287-299.[CrossRef][Medline]
  8. Kelly, W. K., O. A. O’Connor, and P. A. Marks. 2002. Histone deacetylase inhibitors: from target to clinical trials. Expert Opin. Investig. Drugs 11:1695-1713
  9. Melnick, A., and J. D. Licht. 2002. Histone deacetylases as therapeutic targets in hematologic malignancies. Curr. Opin. Hematol. 9:322-332
  10. Gao, L., M. A. Cueto, F. Asselbergs, and P. Atadja. 2002. Cloning and functional characterization of HDAC11, a novel member of the human histone deacetylase family. J. Biol. Chem. 277:25748-25755
Code CY-1158V2
Product Type Kit
Size 100 Assays
Price
$590.21
Qty: