CycLex® SIRT6 Deacetylase Fluorometric Assay Kit Ver.2

This kit is designed for the rapid and sensitive evaluation of SIRT6 inhibitors or activators using recombinant SIRT6 or purified SIRT6.

Specifications:

Description

The CycLex Research Product CycLex SIRT6 Deacetylase Fluorometric Assay Kit detects deacetylase activity of recombinant SIRT6. Primarily, the CycLex Research Product CycLex SIRT6 Deacetylase Fluorometric Assay Kit is designed for the rapid and sensitive evaluation of SIRT6 inhibitors or activators using recombinant SIRT6 or purified SIRT6.

Target: SIRT6
Product Type: Kit
Size: 100 Assays
Research Area / Disease: Cancer
Storage Temperature: • Upon receipt store #5. Developer and #6. Recombinant SIRT6 at -70°C and all other components below -20°C. • Do not expose reagents to excessive light
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Components

  • SIRT6 Assay Buffer
  • Fluoro-Substrate Peptide (0.1 mM)
  • Fluoro-Deacetylated Peptide (0.1 mM)
  • NAD (8 mM)
  • Developer
  • Recombinant SIRT6
  • Stop Solution

References

  1. North, B.J., and Verdin, E.; Sirtuins: SIRT6-related NAD-dependent protein deacetylases. Genome Biol. 5: 224, 2004
  2. Michishita E et al.; Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins. Mol Biol Cell. 16: 4623, 2005
  3. Mostoslavsky R et al.; Genomic instability and aging-like phenotype in the absence of mammalian SIRT6. Cell. 124: 315, 2006
  4. Michishita E et al.; SIRT6 is a histone H3 lysine 9 deacetylase that modulates telomeric chromatin. Nature. 452: 492, 2008
  5. Yang B et al.; The sirtuin SIRT6 deacetylates H3 K56Ac in vivo to promote genomic stability. Cell Cycle. 8: 2662, 2009
  6. Kawahara TL et al.; SIRT6 links histone H3 lysine 9 deacetylation to NF-kappaB-dependent gene expression and organismal life span. Cell. 136: 62, 2009
  7. Van Gool F, et al.; Intracellular NAD levels regulate tumor necrosis factor protein synthesis in a sirtuin-dependent manner. Nat Med. 15: 206, 2009
Code CY-1156V2
Product Type Kit
Size 100 Assays
Price
$566.37
Qty: