CycLex® SIRT3 DeacetylaseFluorometric Assay Kit Ver.2

This kit is designed for the rapid and sensitive evaluation of SIRT3 inhibitors or activators using crude SIRT3 fraction or purified SIRT3.

Specifications:

Description

The CycLex Research Product CycLex SIRT3 Deacetylase Fluorometric Assay Kit detects SIRT3 activity in lysates. Primarily, the CycLex Research Product CycLex SIRT3 Deacetylase Fluorometric Assay Kit is designed for the rapid and sensitive evaluation of SIRT3 inhibitors or activators using crude SIRT3 fraction or purified SIRT3.

Target: SIRT3
Product Type: Kit
Size: 100 Assays
Research Area / Disease: Cancer
Storage Temperature: -70°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Components

SIRT3 Assay Buffer, Fluoro-Substrate Peptide (0.2 mM), Fluoro-Deacetylated Peptide (0.2 mM), NAD (2 mM), Developer, Recombinant SIRT3, Stop Solution.

Citations

  1. Komatsu M et al. NNMT activation can contribute to the development of fatty liver disease by modulating the NAD + metabolism. Sci Rep. 8, 8637 (2018)
  2. Ouyang J et al. SIRT3 Inactivation Promotes Acute Kidney Injury Through Elevated Acetylation of SOD2 and p53. J Surg Res. 233, 221-230 (2019)

References

  1. Frye, R. A. Biochem. Biophys. Res. Commun. 260, 273–279, (1999)
  2. Luo, J., Nikolaev, A. Y., Imai, S., Chen, D., Su, F., Shiloh, A., Guarente, L., and Gu, W. Cell 107, 137–148, (2001)
  3. Langley, E., Pearson, M., Faretta, M., Bauer, U. M., Frye, R. A., Minucci, S., Pelicci, P. G., and Kouzarides, T. EMBO J. 21, 2383–2396, (2002)
  4. Yeung, F., Hoberg, J. E., Ramsey, C. S., Keller, M. D., Jones, D. R., Frye, R. A., and Mayo, M. W. EMBO J. 23, 2369–2380, (2004)
  5. Motta, M. C., Divecha, N., Lemieux, M., Kamel, C., Chen, D., Gu, W., Bultsma, Y., McBurney, M., and Guarente, L. Cell 116, 551–563, (2004)
  6. Brunet, A., Sweeney, L. B., Sturgill, J. F., Chua, K. F., Greer, P. L., Lin, Y., Tran, H., Ross, S. E., Mostoslavsky, R., Cohen, H. Y., Hu, L. S., Cheng, H. L., Jedrychowski, M. P., Gygi, S. P., Sinclair,
  7. D. A., Alt, F. W., and Greenberg, M. E Science 303, 2011–2015, . (2004)
  8. Vaquero, A., Scher, M., Lee, D., Erdjument-Bromage, H., Tempst, P., and Reinberg, D. Mol. Cell 16, 93–105, (2004)
  9. Fulco, M., Schiltz, R. L., Iezzi, S., King, M. T., Zhao, P., Kashiwaya, Y., Hoffman, E., Veech, R. L., and Sartorelli, V. Mol. Cell 12, 51–62, (2003)
  10. North, B. J., Marshall, B. L., Borra, M. T., Denu, J. M., and Verdin, E Mol. Cell 11, 437–444, . (2003)
  11. Dryden, S. C., Nahhas, F. A., Nowak, J. E., Goustin, A. S., and Tainsky, M. A. Mol. Biol. Cell 23, 3173–3185, (2003)
  12. Onyango, P., Celic, I., McCaffery, J. M., Boeke, J. D., and Feinberg, A. P. Proc. Natl. Acad. Sci. U. S. A. 99, 13653–13658, (2002)
  13. Schwer, B., North, B. J., Frye, R. A., Ott, M., and Verdin, E. J. Cell Biol. 158, 647–657, (2002)
  14. Yang, Y. H., Chen, Y. H., Zhang, C. Y., Nimmakayalu, M. A., Ward, D. C., and Weissman, S. Genomics 69, 355–369, (2000)
Code CY-1153V2
Product Type Kit
Size 100 Assays
Price
$566.37
Qty: