CycLex® SIRT2 Deacetylase Fluorometric Assay Kit Ver.2
This kit is designed for the rapid and sensitive evaluation of SIRT2 inhibitors or activators using recombinant SIRT2 or purified SIRT2.
Specifications:
Description
This kit is designed for the rapid and sensitive evaluation of SIRT2 inhibitors or activators using recombinant SIRT2 or purified SIRT2.
| Target: | SIRT2 |
|---|---|
| Product Type: | Kit |
| Size: | 100 Assays |
| Research Area / Disease: | Cell Biology |
| Storage Temperature: | -70°C |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
Components
- SIRT2 Assay Buffer
- Fluoro-Substrate Peptide (0.2 mM)
- Fluoro-Deacetylated Peptide (0.2 mM)
- NAD (8 mM), Developer
- Recombinant SIRT2
- Stop Solution
References
- North, B.J., and Verdin, E.; Sirtuins: SIRT2-related NAD-dependent protein deacetylases. Genome Biol. 5: 224, 2004
- North BJ, Marshall BL, Borra MT, Denu JM, Verdin E; The human SIRT2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase. Mol Cell 11:437-444, 2003
- Hubbert C, Guardiola A, Shao R, Kawaguchi Y, Ito A, Nixon A, Yoshida M, Wang XF, Yao TP; HDAC6 is a microtubule-associated deacetylase. Nature 417: 455-458, 2002
- Hiratsuka M, Inoue T, Toda T, Kimura N, Shirayoshi Y, Kamitani H, Watanabe T, Ohama E, Tahimic
- CG, Kurimasa A, Oshimura M; Proteomics-based identification of differentially expressed genes in human gliomas: down-regulation of SIRT2 gene. BBRC 309: 558-566, 2003
- S Imai, CM Armstrong, M Kaeberlein, and L Guarente; Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase. Nature. 403: 795-800, 2000
- Joseph Landry, Ann Sutton, Stefan T. Tafrov, Ryan C. Heller, John Stebbins, Lorraine Pillus, and Rolf
- Sternglanz; The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases Proc Natl Acad Sci U S A 97: 5807-5811, 2000
- Jeffrey S. Smith, Carrie Baker Brachmann, Ivana Celic, Margaret A. Kenna, Shabazz Muhammad,
- Vincent J. Starai, Jose L. Avalos, Jorge C. Escalante-Semerena, Charles Grubmeyer, Cynthia
- Wolberger, and Jef D. Boeke; A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family Proc Natl Acad Sci U S A 97, 6658-6663, 2000
- H Vaziri, SK Dessain, E Ng Eaton, SI Imai, RA Frye, TK Pandita, L Guarente, and RA Weinberg; hSIR2 (SIRT1) functions as an NAD-dependent p53 deacetylase. Cell. 107: 149-159, 2001
- J Luo, AY Nikolaev, S Imai, D Chen, F Su, A Shiloh, L Guarente, and W Gu; Negative control of p53 by Sir2alpha promotes cell survival under stress. Cell. 107: 137-148, 2001
- E Langley, M Pearson, M Faretta, UM Bauer, RA Frye, S Minucci, PG Pelicci, and T Kouzarides; Human SIR2 deacetylates p53 and antagonizes PML/p53-induced cellular senescence. EMBO J. 21: 2383-2396, 2002
- J Smith; Human Sir2 and the ‘silencing’ of p53 activity. Trends Cell Biol. 12: 404, 2002
- CM Grozinger and SL Schreiber; Deacetylase enzymes: biological functions and the use of small-molecule inhibitors. Chem Biol, 9: 3-16, 2002
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