CycLex® SIRT1/Sir2 DeacetylaseFluorometric Assay Kit Ver.2

This kit is designed for the rapid and sensitive evaluation of SIRT1/Sir2 inhibitors or activators using crude SIRT1/Sir2 fraction or purified SIRT1/Sir2.

Specifications:

Description

The CycLex Research Product CycLex SIRT1/Sir2 Deacetylase Fluorometric Assay Kit detects SIRT1/Sir2 activity in lysates. Primarily, the CycLex Research Product CycLex SIRT1/Sir2 Deacetylase Fluorometric Assay Kit is designed for the rapid and sensitive evaluation of SIRT1/Sir2 inhibitors or activators using crude SIRT1/Sir2 fraction or purified SIRT1/Sir2. Additionally, any cultured primary cell, cell line, or tissue homogenate can be assayed for SIRT1/Sir2 activity with the CycLex Research Product CycLex SIRT1/Sir2 Deacetylase Fluorometric Assay Kit if the appropriate antibody direct against SIRT1 or Sir2 is used for immunoprecipitation.

Target: SIRT1/Sir2
Product Type: Kit
Size: 100 Assays
Research Area / Disease: Cancer
Storage Temperature: • Upon receipt store #5. Developer and #6. Recombinant SIRT1 at -70°C and all other components below -20°C. • Do not expose reagents to excessive light.
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Components

  • SIRT1 Assay Buffer
  • Fluoro-Substrate Peptide (0.2 mM)
  • Fluoro-Deacetylated Peptide (0.2 mM)
  • NAD (2 mM)
  • Developer
  • Recombinant SIRT1
  • Stop Solution

Citations

  1. Shigemoto R et al. 5-Methylmellein is a novel inhibitor of fungal sirtuin and modulates fungal secondary metabolite production. J Gen Appl Microbiol. (2018) In press.
  2. Conti V et al. SIRT1 Activity in Peripheral Blood Mononuclear Cells Correlates with Altered Lung Function in Patients with Chronic Obstructive Pulmonary Disease. Oxid Med Cell Longev. 2018, 9391261 (2018)
  3. Corbi G et al. Phenolic Plant Extracts Induce Sirt1 Activity and Increase Antioxidant Levels in the Rabbit’s Heart and Liver. Oxid Med Cell Longev. 2018, 2731289 (2018)

References

  1. Imai S, et al. Nature. 403: 795-800, 2000
  2. Landry J et al. Proc Natl Acad Sci U S A 97: 5807-5811, 2000
  3. Smith JS, et al. Proc Natl Acad Sci U S A 97: 6658-6663, 2000
  4. Vaziri H, et al. Cell. 107: 149-159, 2001
  5. Luo J et al. Cell. 107, 137-148, 2001
  6. Langley E et al. EMBO J. 21: 2383-2396, 2002
  7. Smith J. Trends Cell Biol. 12: 404, 2002
  8. Grozinger CM, and Schreiber SL. Chem Biol, 9: 3-16, 2002
  9. Sereno, A et al. Antimicrob. Agents Chemother. 49: 808-812, 2005
  10. Nicola Ferrara, et al. Rejuvenation Research 11: 139-150, 2008
  11. Rameshwar U. et al. Chemical Biology & Drug Design 71: 501-506, 2008
  12. Fan Lan, et al. J. Biol. Chem. 283: 27628, 2008
  13. Joana TAVARES et al. Biochemical Journal. 415: 377-86, 2008
  14. Yue Zhao, et al. Mol. Cell. Biol., Dec 2008; 10.1128/MCB.02123-07
Code CY-1151V2
Product Type Kit
Size 100 Assays
Price
$566.37
Qty: