CycLex® SIRT1/Sir2 DeacetylaseFluorometric Assay Kit Ver.2
This kit is designed for the rapid and sensitive evaluation of SIRT1/Sir2 inhibitors or activators using crude SIRT1/Sir2 fraction or purified SIRT1/Sir2.
Specifications:
Description
The CycLex Research Product CycLex SIRT1/Sir2 Deacetylase Fluorometric Assay Kit detects SIRT1/Sir2 activity in lysates. Primarily, the CycLex Research Product CycLex SIRT1/Sir2 Deacetylase Fluorometric Assay Kit is designed for the rapid and sensitive evaluation of SIRT1/Sir2 inhibitors or activators using crude SIRT1/Sir2 fraction or purified SIRT1/Sir2. Additionally, any cultured primary cell, cell line, or tissue homogenate can be assayed for SIRT1/Sir2 activity with the CycLex Research Product CycLex SIRT1/Sir2 Deacetylase Fluorometric Assay Kit if the appropriate antibody direct against SIRT1 or Sir2 is used for immunoprecipitation.
| Target: | SIRT1/Sir2 |
|---|---|
| Product Type: | Kit |
| Size: | 100 Assays |
| Research Area / Disease: | Cancer |
| Storage Temperature: | • Upon receipt store #5. Developer and #6. Recombinant SIRT1 at -70°C and all other components below -20°C. • Do not expose reagents to excessive light. |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
Components
- SIRT1 Assay Buffer
- Fluoro-Substrate Peptide (0.2 mM)
- Fluoro-Deacetylated Peptide (0.2 mM)
- NAD (2 mM)
- Developer
- Recombinant SIRT1
- Stop Solution
Citations
- Shigemoto R et al. 5-Methylmellein is a novel inhibitor of fungal sirtuin and modulates fungal secondary metabolite production. J Gen Appl Microbiol. (2018) In press.
- Conti V et al. SIRT1 Activity in Peripheral Blood Mononuclear Cells Correlates with Altered Lung Function in Patients with Chronic Obstructive Pulmonary Disease. Oxid Med Cell Longev. 2018, 9391261 (2018)
- Corbi G et al. Phenolic Plant Extracts Induce Sirt1 Activity and Increase Antioxidant Levels in the Rabbit’s Heart and Liver. Oxid Med Cell Longev. 2018, 2731289 (2018)
References
- Imai S, et al. Nature. 403: 795-800, 2000
- Landry J et al. Proc Natl Acad Sci U S A 97: 5807-5811, 2000
- Smith JS, et al. Proc Natl Acad Sci U S A 97: 6658-6663, 2000
- Vaziri H, et al. Cell. 107: 149-159, 2001
- Luo J et al. Cell. 107, 137-148, 2001
- Langley E et al. EMBO J. 21: 2383-2396, 2002
- Smith J. Trends Cell Biol. 12: 404, 2002
- Grozinger CM, and Schreiber SL. Chem Biol, 9: 3-16, 2002
- Sereno, A et al. Antimicrob. Agents Chemother. 49: 808-812, 2005
- Nicola Ferrara, et al. Rejuvenation Research 11: 139-150, 2008
- Rameshwar U. et al. Chemical Biology & Drug Design 71: 501-506, 2008
- Fan Lan, et al. J. Biol. Chem. 283: 27628, 2008
- Joana TAVARES et al. Biochemical Journal. 415: 377-86, 2008
- Yue Zhao, et al. Mol. Cell. Biol., Dec 2008; 10.1128/MCB.02123-07
