CycLex® HDACs DeacetylaseFluorometric Assay Kit Ver.2

This kit is designed for the rapid and sensitive evaluation of HDAC inhibitors using crude HDAC fraction.

Specifications:

Description

The CycLex Research Product CycLex HDACs Deacetylase Fluorometric Assay Kit detects HDAC activity in lysates. Primarily, the CycLex Research Product CycLex HDACs Deacetylase Fluorometric Assay Kit is designed for the rapid and sensitive evaluation of HDAC inhibitors using crude HDAC fraction. Additionally, any cultured primary cell, cell line, or tissue homogenate can be assayed for HDAC activity with the CycLex Research Product CycLex HDACs Deacetylase Fluorometric Assay Kit if the appropriate dose of HDAC specific inhibitor e.g. Trichostatin A is used.

Target: HDACs
Product Type: Kit
Size: 100 Assays
Research Area / Disease: Cancer
Storage Temperature: -70°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Components

  • HDAC Assay Buffer
  • Fluoro-Substrate Peptide (0.2 mM)
  • Fluoro-Deacetylated Peptide (0.2 mM)
  • Trichostatin A (10 mu;M)
  • Developer
  • HDACs
  • Stop Solution

References

  1. Davie, J. R & Chadee, D. N. J. Cell Biochem. (Suppl.) 30-31, 203-213, 1998
  2. Kouzarides, T. Curr. Opin. Genet. Dev. 9, 40-84, 1999
  3. Fenrick, R. & Hiebert, S.W. J. Cell Biochem. (Suppl.) 30-31, 194-202, 1998
  4. Yoshida, M., Horinouchi, S. & Beppu, T. Bioassays 17, 423-430, 1995
  5. Richon, V. M. et al. Proc. Natl.Acad. Sci. USA 93, 5705-5708, 1996
  6. Richon, V. M. et al. Proc. Natl.Acad. Sci. USA 95 3003-3007, 1998
  7. Cohen, L. et al. Proc. AACR 39, 108, abstr. 736, 1998
  8. Desai, D., El-Bayoumy, K. & Amin, S. Proc. AACR 40, 2396, abstr. 362, 1999
  9. Laherty, C. D., Yang, W-M. et al Cell 89, 349-356, 1997
  10. Hassig, C., Fleischer, T. C. et al Cell 89, 341-347, 1997
  11. Hoffmann, K., Grosch, G. & Jung, M Nucleic Acids Res. 27, 2057-2058, 1999
Code CY-1150V2
Product Type Kit
Size 100 Assays
Price
$536.56
Qty: