CycLex® HDACs DeacetylaseFluorometric Assay Kit Ver.2
This kit is designed for the rapid and sensitive evaluation of HDAC inhibitors using crude HDAC fraction.
Specifications:
Description
The CycLex Research Product CycLex HDACs Deacetylase Fluorometric Assay Kit detects HDAC activity in lysates. Primarily, the CycLex Research Product CycLex HDACs Deacetylase Fluorometric Assay Kit is designed for the rapid and sensitive evaluation of HDAC inhibitors using crude HDAC fraction. Additionally, any cultured primary cell, cell line, or tissue homogenate can be assayed for HDAC activity with the CycLex Research Product CycLex HDACs Deacetylase Fluorometric Assay Kit if the appropriate dose of HDAC specific inhibitor e.g. Trichostatin A is used.
| Target: | HDACs |
|---|---|
| Product Type: | Kit |
| Size: | 100 Assays |
| Research Area / Disease: | Cancer |
| Storage Temperature: | -70°C |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
Components
- HDAC Assay Buffer
- Fluoro-Substrate Peptide (0.2 mM)
- Fluoro-Deacetylated Peptide (0.2 mM)
- Trichostatin A (10 mu;M)
- Developer
- HDACs
- Stop Solution
References
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- Richon, V. M. et al. Proc. Natl.Acad. Sci. USA 93, 5705-5708, 1996
- Richon, V. M. et al. Proc. Natl.Acad. Sci. USA 95 3003-3007, 1998
- Cohen, L. et al. Proc. AACR 39, 108, abstr. 736, 1998
- Desai, D., El-Bayoumy, K. & Amin, S. Proc. AACR 40, 2396, abstr. 362, 1999
- Laherty, C. D., Yang, W-M. et al Cell 89, 349-356, 1997
- Hassig, C., Fleischer, T. C. et al Cell 89, 341-347, 1997
- Hoffmann, K., Grosch, G. & Jung, M Nucleic Acids Res. 27, 2057-2058, 1999
