CycLex® Cellular Histone Acetylation Assay Kit

CycLex Cellular Histone Acetylation Assay Kit.

Specifications:

Components

  • Microplate
  • 100X Trichostatin A
  • 10X Wash Buffer
  • Blocking Reagent
  • Primary Antibody Solution(Anti-Acetylated Histone/p53-K382 Monoclonal Antibody TM-5C5)
  • Secondary Antibody Solution (HRP conjugated Anti-Mouse IgG)
  • Substrate Reagent, Stop Solution

Description

The CycLex Cellular Histone Acetylation Assay Kit is used for the semi-quantitative measurement of histone acetylation level in situ by means of cell-based ELISA It can be used for 2 x 96 wells.

Target: Cellular Histone Acetylation
Product Type: Kit
Size: 2 x 96 Wells
Research Area / Disease: Cancer
Storage Temperature: 4°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Citations

  1. K Reed , S L Hembruff , J A Sprowl & A M Parissenti; The temporal relationship between ABCB1 promoter hypomethylation, ABCB1 expression and acquisition of drug resistance. The Pharmacogenomics Journal, 10: 489-504, 2010,
  2. Nishino T, Wang C, Mochizuki-Kashio M, Osawa M, Nakauchi H, Iwama A.; Ex vivo expansion of human hematopoietic stem cells by garcinol, a potent inhibitor of histone acetyltransferase. PLoS One. 6:e24298. 2011,
  3. Venturelli S et al. Resveratrol as a pan-HDAC inhibitor alters the acetylation status of histone proteins in human-derived hepatoblastoma cells. PLoS One. 8, e73097 (2013)

References

  1. Davie, J. R & Chadee, D. N. J. Cell Biochem. (Suppl.) 30-31, 203-213, 1998
  2. Kouzarides, T. Curr. Opin. Genet. Dev. 9, 40-84, 1999
  3. Fenrick, R. & Hiebert, S.W. J. Cell Biochem. (Suppl.) 30-31, 194-202, 1998
  4. Yoshida, M., Horinouchi, S. & Beppu, T. Bioassays 17, 423-430, 1995
  5. Richon, V. M. et al. Proc. Natl. Acad. Sci. USA 93, 5705-5708, 1996
  6. Richon, V. M. et al. Proc. Natl. Acad. Sci. USA 95 3003-3007, 1998
  7. Cohen, L. et al. Proc. AACR 39, 108, abstr. 736, 1998
  8. Desai, D., El-Bayoumy, K. & Amin, S. Proc. AACR 40, 2396, abstr. 362, 1999
  9. Laherty, C. D., Yang, W-M. et al Cell 89, 349-356, 1997
  10. Hassig, C., Fleischer, T. C. et al Cell 89, 341-347, 1997
  11. Hoffmann, K., Grosch, G. & Jung, M Nucleic Acids Res. 27, 2057-2058, 1999
Code CY-1140
Product Type Kit
Size 2 x 96 Wells
Price
$522.36
Qty: