CycLex® Cellular Histone Acetylation Assay Kit

CycLex Cellular Histone Acetylation Assay Kit.

Specifications:

Description

The CycLex Cellular Histone Acetylation Assay Kit is used for the semi-quantitative measurement of histone acetylation level in situ by means of cell-based ELISA It can be used for 2 x 96 wells.

Target: Cellular Histone Acetylation
Product Type: Kit
Size: 2 x 96 Wells
Research Area / Disease: Cancer
Storage Temperature: 4°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Components

  • Microplate
  • 100X Trichostatin A
  • 10X Wash Buffer
  • Blocking Reagent
  • Primary Antibody Solution(Anti-Acetylated Histone/p53-K382 Monoclonal Antibody TM-5C5)
  • Secondary Antibody Solution (HRP conjugated Anti-Mouse IgG)
  • Substrate Reagent, Stop Solution

Citations

  1. Okabe, Seiichi, Yuko Tanaka, and Akihiko Gotoh. “Targeting phosphoinositide 3-kinases and histone deacetylases in multiple myeloma.” Experimental Hematology & Oncology 10.1 (2021): 1-12.
  2. Okabe, Seiichi, et al. “Effect of dual inhibition of histone deacetylase and phosphatidylinositol-3 kinase in Philadelphia chromosome-positive leukemia cells.” Cancer Chemotherapy and Pharmacology 85.2 (2020): 401-412.
  3. Shan, Changliang, et al. “4-hydroxyphenylpyruvate dioxygenase promotes lung cancer growth via pentose phosphate pathway (PPP) flux mediated by LKB1-AMPK/HDAC10/G6PD axis.” Cell death & disease 10.7 (2019): 1-13.
  4. Nishino T, Wang C, Mochizuki-Kashio M, Osawa M, Nakauchi H, Iwama A.; Ex vivo expansion of human hematopoietic stem cells by garcinol, a potent inhibitor of histone acetyltransferase. PLoS One. 6:e24298. 2011,
  5. K Reed , S L Hembruff , J A Sprowl & A M Parissenti; The temporal relationship between ABCB1 promoter hypomethylation, ABCB1 expression and acquisition of drug resistance. The Pharmacogenomics Journal, 10: 489-504, 2010,

References

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