CD1d is a membrane protein non-covalently bonded to β2-microglobulin (β2m) and shows high homology between human and mouse. CD1d can present α-galactosylceramide (α-GalCer), a glycolipid extracted and isolated from the marine sponge, and this complex can activate human and murine CD1d-restricted NKT cells. CD1d tetramer is a reagent prepared by tetramerization of complexes of CD1d and β2m by PE- or APC- labeled streptavidin. Binding this reagent to α-GalCer enables highly sensitive detection of CD1d-restricted NKT cells and can be combined with antibodies to study NKT cell function by flow cytometry.
Dissolving and loading α-GalCer on CD1d tetramer
|Product||α-GalCer unloaded||α-GalCer loaded|
|MBL code no.||MBL code no.|
Human CD1d Tetramer-PE
Human CD1d Tetramer-APC
Mouse CD1d Tetramer-PE
Mouse CD1d Tetramer-APC
Detection of human NKT cells with CD1d tetramer
- Stephane S, et al., J. Immunol. Methods 268: 107-121 (2002)
- Matsuda JL, et al., J. Exp. Med. 192: 741-754 (2000)
- Benlagha K, et al., J. Exp. Med. 191: 1895-1903 (2000)
For Research Use Only. Not for use in diagnostic procedures.
Learn more about the other types of MHC Molecules