Coxsackie B Screen (each well contains a mixture of B1, B2, B3, B4, B5 and B6) Substrate Slide

  • Applications
    • IF
  • Code # CB-3306
  • Size 6 wells
  • Price Call for Price
Specifications

Background

Coxsackieviruses were first recovered in 1949 in suckling mice inoculated with the fecal extracts from two children with paralytic poliomyelitis-like syndrome. Currently, there are six Coxsackievirus B and twenty-four Coxsackievirus A serotypes recognized. The virus is spread primarily by fecal-oral contamination, although respiratory transmission also occurs. The virus initially colonizes the alimentary tract, growing in the lymphoid tissues of the nasopharynx and intestinal tract. Viremia subsequently occurs allowing secondary localization in other viscera and lymphoid tissues. Infection is often asymptomatic (30-90%), but a wide variety of notable syndromes may also occur such as paralytic disease, aseptic meningitis, encephalitis, pleurodynia, pericarditis, herpangina and lymphonodular pharyngitis. The most common clinical picture is that of an undifferentiated febrile illness with malaise, headache, myalgia and sore throat.

  • Application:
    IF
  • Components:
    MBL Bion COXSACKIEVIRUS B ANTIGEN SUBSTRATE SLIDES are individually foil-wrapped slides of six wells with a mixture of Coxsackievirus B (1-6; NIH strains) infected and uninfected A549 cells fixed onto each well. Each well contains an average on 20-30% infected cells per 200X field.
  • Description:

    The MBL Bion COXSACKIEVIRUS B ANTIGEN SUBSTRATE SLIDES may be utilized in the indirect fluorescent antibody assay. Each lot is tested with a panel of titered sera to ensure sensitivity lot to lot; both positive and negative cells are in each well to provide a built-in control.

    INTENDED USE

    The MBL Bion COXSACKIEVIRUS B ANTIGEN SUBSTRATE SLIDES may be used as the antigenic substrate in indirect fluorescent antibody assays for the qualitative and/or semi-quantitative determination of Coxsackievirus B antibodies in human serum. MBL Bion COXSACKIEVIRUS B ANTIGEN SUBSTRATE SLIDES are intended for use as an aid in the diagnosis of active infection and as a determination of immunological experience with Coxsackievirus B.

  • Product Type:
    Antigen Substrate Slide
  • Research Area:
    Infectious Disease
  • Short Description:

    The MBL Bion COXSACKIEVIRUS B ANTIGEN SUBSTRATE SLIDES may be used as the antigenic substrate in indirect fluorescent antibody assays for the qualitative and/or semi-quantitative determination of Coxsackievirus B antibodies in human serum. 

  • Size:
    6 wells
References
  1. “Coxsackievirus.” Mosby’s Medical, Nursing and Allied Health dictionary, 1994 ed.
  2. Lennette, Edwin, H., Albert Balows, William J. Hausler Jr., and Joseph P. Truant, ed. Manual of Clinical Microbiology. 3rd ed. Washington, D.C.: American Society for Microbiology, p. 823, 1980.
  3. Henry, John B MD, ed. Clinical Diagnosis and Management by Laboratory Methods, 16th ed. 2 vols. Philadelphia: W.B. Saunders Co., 1849-1851, 1979.
  4. Murray, Brian J. M.D. “Complications Following Coxsackievirus B Infection.” Amer. Fam. Physician; 38:115-118, 1988.
  5. Isacsohn, M., A. Eidelman, M. Kaplan, A. Goren, B. Rudensky, R. Handsher, and Y. Barak. “Neonatal Coxsackievirus Group B Infections: Experience of a Single Department of Neonatology.” Israel J. Med. Sciences; 30:371-374, 1994.
  6. Tam, P., A. Schmidt, S. Ytterberg, and R. Messner. “Viral Persistence During the Developmental Phase of Coxsackie B1-Induced Murine Polymyositis.” J. Virol;65:6654-6660, 1991.
  7. Bendinelli, M., and Herman Friedman, ed. Coxsackieviruses: A General Update. New York: Plenum Press, pp. 3, 83-85, 215-216, 1988.
  8. Grandien, M., M. Forsgren, and A. Ehrnst, Enteroviruses and Reoviruses, in Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections, 6th ed., N.J. Schmidt and R.W. Emmons, (eds.), American Public Health Association, Washington, D.C., 513-560, 1989.
  9. Rotbart, H.A., Enteroviruses, in Manual of Clinical Microbiology, 6th ed., P.R. Murray, E.J.  Baron, et. al.(eds.), ASM Press, Washington, D.C., 1004-1009, 1995.
  10. Menegus, M.A., Enteroviruses, in Manual of Clinical Microbiology, 5th ed., A. Balows, Q.J. Hansler, Jr., et. al.(eds.), American Society for Microbiology, Washington, D.C., 943-946, 1991.
  11. Weller, T.H., A.H. Coons, Fluorescent Antibody Studies with Agents of Varicella and Herpes Zoster Propagated In Vitro, Proc. Soc. Exp. Biol. Med., 86:789-794, 1954.
  12. Riggs, J.L., R.J. Siewald, J.H. Burckhalter, C.M. Downs, T.G. Metcalf, Isothiocyanate Compounds as Fluorescent Labeling Agents for Immune Serum, Am. J. Pathol. 34:1081-1097, 1958.
  13. Goldman, M.: Fluorescent Antibody Methods. New York, Academic Press, 1968.
  14. Kawamura, A. Jr.: Fluorescent Antibody Techniques and Their Application. Baltimore, University Park Press, 1969.
  15. Nairn, R.C.: Fluorescent Protein Tracing, Baltimore, Williams and Wilkins, 3rd Ed., 1969.
  16. Johnson, R.B., and R. Libby, Separation of Immunoglobulin M (IgM) Essentially Free of IgG From Serum in Systems Requiring Assay of IgM-Type Antibodies Without Interference From Rheumatoid Factor, J. Clin. Micro. 12:451-454, 1980.
  17. Gispen, R., J. Nagel, B. Brand-Saathof, S. DeGraff, Immunofluorescence Test for IgM Rubella Antibodies in Whole Serum After Absorption with Specific Anti-gamma Fc, Clin. Exp. Immunol., 22:431-437, 1975.
  18. Joassin, L., M. Reginster, Elimination of Nonspecific Cytomegalovirus Immunoglobulin M Activities in the Enzyme-Linked Immunosorbent Assay by Using Antihuman Immunoglobulin G, J. Clin. Microbiol. 23:576-581, 1986.
  19. Lyerla, H.C., F.T. Forrester, The Immunofluorescence (IF) Test, in: Immunofluorescence Methods in Virology, USDHHS, Georgia, 71-81, 1979.
  20. Holborow, E.J., D.M. Weir, G.D. Johnson, A Serum Factor in Lupus Erythematosus with Affinity for Tissue Nuclei, Br. Med. J., 11:732-734, 1957.
  21. Berg, P.A., I. Roitt, D. Doniach, H.M. Cooper, Mitochondrial Antibodies in Primary Biliary Cirrhosis, Immunol. 17:281-293, 1969.
  22. Gardner, P.S., J. McQuillin, Rapid Virus Diagnosis: Application of Immunofluorescence, In: Detection of Virus Specific IgM by Immunofluorescence, Butterworth, Boston, 259-287, 1980.
  23. Schmidt, N.J., Update on Class-specific Viral Antibody Assays, Clinical Immunology Newsletter, 5 (6) :81-85, June, 1984.
  24. Chernesky, M.A., C.G. Ray, T.F. Smith, Laboratory Diagnosis of Viral Infections, Cumitech 15, ASM, Washington, D.C., March 1982.
  25. Data on file, MBL Bion, Des Plaines, IL.
  26. Bowman, J., B. Nilsson, and P. Juto., Serum IgA, IgG and IgM Responses to Different Enteroviruses as Measured by Coxsackie B5-Based Indirect ELISA.” J. of Med. Virol.; 38:32-35, 1992.