Borrelia burgdorferi Substrate Slide

  • Applications
    • IF
  • Code # BB-6112
  • Size 12 wells
  • Price Call for Price
Specifications

Background

Lyme borreliosis (Lyme disease) is caused by the spirochete Borrelia burgdorferi sensu lato and transmitted primarily through the bite of the deer tick, Ixodes scapularis in the Midwest and Northeast, or Ixodes pacificus in the West. Lyme borreliosis has become the most commonly diagnosed tick-borne illness in the United States. Immunologic response in individuals with early, primary disease tend to be directed to 23, 39 and 41-kDa flagellin antigen and are of the IgM class. These antibodies may be detectable within one to two weeks following the tick bite, but are generally delayed for four to six weeks. IgM antibodies may persist for months even if appropriate antimicrobial agents are administered. IgG immunologic responses appear to be directed to other immunogens of B. burgdorferi during later stages of Lyme borreliosis.

  • Application:
    IF
  • Components:

    MBL Bion BORRELIA BURGDORFERI ANTIGEN SUBSTRATE SLIDES are individually foil-wrapped twelve well slides with a suspension of Borrelia burgdorferi microorganisms (Strain B31) fixed onto each well.

  • Description:

    The MBL Bion BORRELIA BURGDORFERI ANTIGEN SUBSTRATE SLIDES may be utilized in the indirect fluorescent antibody assay method. Each lot is tested with a panel of titered sera to ensure sensitivity lot to lot.

    INTENDED USE

    The MBL Bion BORRELIA BURGDORFERI ANTIGEN SUBSTRATE SLIDES may be used as the antigenic substrate in indirect fluorescent antibody assays for the qualitative and/or semi-quantitative presumptive determination of Borrelia burgdorferi IgG or IgM antibodies in human serum. MBL Bion BORRELIA BURGDORFERI ANTIGEN SUBSTRATE SLIDES are intended for use as an aid in the diagnosis of active infection, reinfection, or reactivation of the latent microbe and as a determination of immunological experience with Borrelia burgdorferi. Results should be supplemented by a second assay method, such as Western Blot, to provide supplementary serological evidence of the presence or absence of a Borrelia burgdorferi infection.

  • Product Type:
    Antigen Substrate Slide
  • Research Area:
    Infectious Disease
  • Short Description:

    The MBL Bion BORRELIA BURGDORFERI ANTIGEN SUBSTRATE SLIDES may be used as the antigenic substrate in indirect fluorescent antibody assays for the qualitative and/or semi-quantitative presumptive determination of Borrelia burgdorferi IgG or IgM antibodies in human serum.

  • Size:
    12 wells
References
  1. CDC Update: Lyme disease and cases occurring during pregnancy-United States. Morbid. Mortal. Weekly Rep. 34:376-384, 1985.
  2. Habicht, G.S., G. Beck, and J.L. Benach, Lyme Disease, Scientific American 257:78-83, 1987.
  3. Steere, A.C., S.E. Malawista et al., The Clinical Spectrum and Treatment of Lyme Disease, Yale J. Biol. Med. 57:453-461, 1984.
  4. Steere, A.C., R.L. Gredzicki, et al., The spirochetal etiology of Lyme disease. N. Engl. J. Med. 308:733-740, 1983.
  5. Barbour, A.G., Laboratory Aspects of Lyme Borreliosis, Clin. Micro. Rev. 1:399-414, 1988.
  6. Hunter, E.F., H. Russell, C.E. Farshy, et al., Evaluation of Sera from Patients with Lyme Disease in the Fluorescent Treponemal Antibody Absorption Test for Syphilis, Sex. Trans. Dis. 13:232-236, 1986.
  7. Mitchell, P.D., Marshfield Medical Center Laboratory, Marshfield, WI, Personal communication, 1988.
  8. Steere, A.C., W.P. Batsford, et al., Lyme Carditis: Cardiac Abnormalities of Lyme Disease, Ann. Internal. Med. 93:8-16, 1980.
  9. Johnson, R.C., Lyme Borreliosis: A Disease That Has Come Into Its Own, Lab. Management 26:34-40, 1988.
  10. Craft, J.E., R.L. Grodzicki and A.C. Steere, Antibody response in Lyme disease: Evolution of Diagnostic Tests, J. Infect. Dis. 149:789-795, 1984.
  11. Ackermann, R., J. Kabatzki, H.P. Boisten et al., Ixodes ricinus spirochete and European erythema chronicum migrans disease, Yale J. Biol. Med. 57:573-580, 1984.
  12. Magnarelli, L.A., J.A. Meegan, J.F. Anderson, and W.A. Chappell, Comparison of an indirect fluorescent antibody test with an enzyme-linked immunosorbent assay for serological studies of Lyme disease, J. Clin. Micro. 20:181-184, 1984.
  13. Russell, H., J.S. Sampson, G.P. Schmid et al., Enzyme-linked immunosorbent assay and indirect immunofluorescence assay for Lyme disease, J. Infect. Dis. 149:465-470, 1984.
  14. Pfister, H.W., U. Neubert, B. Wilske, et al., Reinfection with Borrelia burgdorferi, Lancet ii:984-985, 1986.
  15. Mertz, L.E., G.H. Wobig, J. Duffy, and J.A. Katzman, Ticks, Spirochetes and new diagnostic tests for Lyme disease, Mayo Clin. Proc. 60: 402-406, 1985.
  16. Weller, T.H., A.H. Coons, Fluorescent Antibody Studies with Agents of Varicella and Herpes Zoster Propagated In Vitro, Proc. Soc. Exp. Biol. Med., 86:789-794, 1954.
  17. Riggs, J.L., R.J. Siewald, J.H. Burckhalter, C.M. Downs, T.G. Metcalf, Isothiocyanate Compounds as Fluorescent Labeling Agents for Immune Serum, Am. J. Pathol. 34:1081-1097, 1958.
  18. Goldman, M., Fluorescent Antibody Methods, New York, Academic Press, 1968.
  19. Kawamura, A. Jr., Fluorescent Antibody Techniques and Their Application, Baltimore, University Park Press, 1969.
  20. Nairn, R.C., Fluorescent Protein Tracing, Baltimore, Williams and Wilkins, 3rd Ed., 1969.
  21. Johnson, R.B., and R. Libby, Separation of Immunoglobulin M (IgM) Essentially Free of IgG From Serum in Systems Requiring Assay of IgM-Type Antibodies Without  Interference From Rheumatoid Factor, J. Clin. Micro. 12:451-454, 1980.
  22. Gispen, R., J. Nagel, B. Brand-Saathof, S. DeGraff, Immunofluorescence Test for IgM Rubella Antibodies in Whole Serum After Absorption with Specific Anti-gamma Fc, Clin. Exp. Immunol., 22:431-437, 1975.
  23. Joassin, L., M. Reginster, Elimination of Nonspecific Cytomegalovirus Immunoglobulin M Activities in the Enzyme-Linked Immunosorbent Assay by Using Antihuman Immunoglobulin G., J. Clin. Micro. 23:576-581, 1986.
  24. Lyerla, H.C., F.T. Forrester, The Immunofluorescence (IF) Test, in: Immunofluorescence Methods in Virology, USDHHS, Georgia, 71-81, 1979.
  25. Data on file, MBL Bion, Des Plaines, IL.
  26. Chernesky, M.A., C.G. Ray, T.F. Smith, Laboratory Diagnosis of Viral Infections, Cumitech 15, ASM, Washington, D.C., March 1982.
  27. Gardner, P.S., J. McQuillin, Rapid Virus Diagnosis: Application of Immunofluorescence, In: Detection of Virus specific IgM by Immunofluorescence, Butterworth, Boston, 259-287, 1980.
  28. Mitchell, P.D., K.D. Reed, T.L. Aspeslet, M.F. Vandermause, J.W. Melski, Comparison of Four Immunoserologic Assays for Detection of Antibodies to Borrelia burgdorferi in Patients with Culture-Positive Erythema Migrans, J. Clin. Micro.,32:1958-1962, 1994.
  29. “Recommendations for Test Performance and Interpretation from the Second National Conference on Serologic Diagnosis of Lyme Disease”, Centers for Disease Control and Prevention, MMWR 1995; 44;590-591.