Recombinant Human IL-18

IL-18 Recombinant Protein



Interleukin 18 (IL-18) is an 18-kDa cytokine which identified as a costimulatory factor for production of interferon- (IFN-gamma) in response to toxic shock and shares functional similarities with IL-12. IL-18
is synthesized as a precursor 24-kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T
or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages and enhances NK activity and B cell maturation, proliferation and Ig secretion. IFN- also induces expression of MHC class I and II antigens and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce
IFN-gamma. Pleiotropic effects of IL-18 have also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.


Target: IL-18
Product Type: Cytokine
Size: 25 µg
Research Area / Disease: Immunology
Species Reactivity: Human
Purity: Greater than 90% purity as confirmed on SDS-PAGE by Coomassie brilliant blue staining.
Endotoxin Level: Less than 0.1 ng per 1 g of recombinant human IL-18 protein, when measured by the LAL assay.
Formulation: 25 μg of lyophilized human IL-18
Reconstitution: Reconstitute with 250 μL of ice-cold distilled water on ice. The concentration will be 25 μg of human IL-18 in 250 μL volume of PBS containing 0.1% BSA and 1% sucrose.


See even more publications

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