Recombinant Human IL-18

  • Target IL-18
  • Code # B001-5
  • Size 25 µg
  • Price
    $306.33
Specifications

Alternative Names

interleukin 18 (interferon-γ-inducing factor)

Background

Interleukin 18 (IL-18) is an 18-kDa cytokine which identified as a costimulatory factor for production of interferon-g (IFN-g) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24-kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-g is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-g activates macrophages and enhances NK activity and B cell maturation, proliferation and Ig secretion. IFN-g also induces expression of MHC class I and II antigens and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-g. Pleiotropic effects of IL-18 have also been reported, such as, enhancement production of IFN-g and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-g in T cells, enhancement of Fas ligand expression by Th1 cells.
  • Description:
    IL-18 Protein of 25 µg.
  • Endotoxin Level:
    #REF!
  • Formulation:
    25 ug in PBS containing 0.1% BSAand 1% sucrose. Reconstitute in 250 uL of ice-colddistilled water on ice.
  • Molecular Weight:
    18 kDa
  • Product Type:
    Cytokine
  • Purity:
    Greater than 90% purity as confirmed on SDS-PAGE by Coomassie brilliant blue staining.
  • Research Area:
    Immunology
  • Short Description:
    IL-18 Protein.
  • Size:
    25 µg
  • Storage Temperature:
    -20°C. Please refer to datasheet for additional information
  • Target:
    IL-18
Citations
  1. Reeves, E. P., et al., J. Immunol. 184, 1642-1652 (2010)
  2. Guia, S., et al., Blood 111, 5008-5016 (2008)
  3. Takahashi, H. K., et al., J. Leukoc. Biol. 77, 400-407 (2005)
  4. Hata, H., et al., Int. Immunol16, 1733-1739 (2004)
  5. Takahashi, H. K., et al., J. Immunol168, 4446-4454 (2002
  6. Ni J, Miller M, Stojanovic A, Garbi N, Cerwenka A. Sustained effector function of IL-12/15/18-preactivated NK cells against established tumors. J Exp Med. 2012;209(13):2351-65.
  7. Romee R, Schneider SE, Leong JW, et al. Cytokine activation induces human memory-like NK cells. Blood. 2012;120(24):4751-60.
  8. Ndhlovu LC, Lopez-vergès S, Barbour JD, et al. Tim-3 marks human natural killer cell maturation and suppresses cell-mediated cytotoxicity. Blood. 2012;119(16):3734-43.
  9. Matsunaga H, Hokari R, Ueda T, et al. Physiological stress exacerbates murine colitis by enhancing proinflammatory cytokine expression that is dependent on IL-18. Am J Physiol Gastrointest Liver Physiol. 2011;301(3):G555-64.
  10. Reeves EP, Williamson M, Byrne B, et al. IL-8 dictates glycosaminoglycan binding and stability of IL-18 in cystic fibrosis. J Immunol. 2010;184(3):1642-52.
  11. Van de wetering D, De paus RA, Van dissel JT, Van de vosse E. IL-23 modulates CD56+/CD3- NK cell and CD56+/CD3+ NK-like T cell function differentially from IL-12. Int Immunol. 2009;21(2):145-53.
  12. Bachmann M, Dragoi C, Poleganov MA, Pfeilschifter J, Mühl H. Interleukin-18 directly activates T-bet expression and function via p38 mitogen-activated protein kinase and nuclear factor-kappaB in acute myeloid leukemia-derived predendritic KG-1 cells. Mol Cancer Ther. 2007;6(2):723-31.
  13. Omoto Y, Tokime K, Yamanaka K, et al. Human mast cell chymase cleaves pro-IL-18 and generates a novel and biologically active IL-18 fragment. J Immunol. 2006;177(12):8315-9.
  14. Elbim C, Guichard C, Dang PM, et al. Interleukin-18 primes the oxidative burst of neutrophils in response to formyl-peptides: role of cytochrome b558 translocation and N-formyl peptide receptor endocytosis. Clin Diagn Lab Immunol. 2005;12(3):436-46.
  15. Dai SM, Nishioka K, Yudoh K. Interleukin (IL) 18 stimulates osteoclast formation through synovial T cells in rheumatoid arthritis: comparison with IL1 beta and tumour necrosis factor alpha. Ann Rheum Dis. 2004;63(11):1379-86.
  16. Yoshino O, Osuga Y, Koga K, et al. Evidence for the expression of interleukin (IL)-18, IL-18 receptor and IL-18 binding protein in the human endometrium. Mol Hum Reprod. 2001;7(7):649-54.
  17. Vankayalapati R, Wizel B, Weis SE, Samten B, Girard WM, Barnes PF. Production of interleukin-18 in human tuberculosis. J Infect Dis. 2000;182(1):234-9.
References
  1. Tao, D., et al., Cell Immunol. 173, 230-235 (1998)
  2. Ushio, S., et al., J. Immunol. 156, 4274-4279 (1996)
  3. Micallef, M., et al., Eur. J. Immunol. 26, 1647-1651 (1996)
  4. Okamura, H., et al., Nature 378, 88-91 (1995)