Anti-LC3 (Human) mAb (Monoclonal Antibody)

LC3 Monoclonal Antibody.

Specifications:

Description

This LC3 antibody is validated for multiple applications (WB, ICC, IH, IP, FCM, Immuno-EM) and has over 25 citations. This is a monoclonal antibody of 200 μg targeting LC3 for FCM, ICC, IP, WB.

More information about LC3 and autophagy can be found in our Blog. In addition you may download our Autophagy Pathway Poster.

Target: LC3
Product Type: Antibody
Size: 100 uL
Application: FCM, ICC, IH, Immuno-EM, IP, WB
Research Area / Disease: Autophagy
Conjugate: Unlabeled
Antibody Type: Monoclonal
Clone Number: 4E12
Concentration: 2 mg/mL
Formulation: 200 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Isotype: IgG1 ĸ
Immunogen: Recombinant human LC3 (MAP1LC3B: 1-120 a.a.)
Host Species: Mouse
Species Reactivity: Hamster, Human, Mouse, Rat
Source: This antibody was purified from hybridoma (clone 4E12) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with the recombinant human LC3 [MAP1LC3B (1-120 aa)].
Reactivity: This antibody reacts with LC3 (MAP1LC3A, B) on Immunocytochemistry, Western blotting and Immunoprecipitation.
Gene ID Human:

8163184557

Gene ID Mouse:

67443, 66734

Gene ID Rat:

64862362245

Storage Temperature: -20°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Citations

  1. Cai T et al. Deletion of Ia-2 and/or Ia-2?A in mice decreases insulin secretion by reducing the number of dense core vesicles. Diabetologia. 54, 2347-57 (2011),
  2. Chalazonitis A et al. Homeodomain interacting protein kinase 2 regulates postnatal development of enteric dopaminergic neurons and glia via BMP signaling. J Neurosci. 31, 13746-57 (2011),
  3. Eng KE et al. A novel quantitative flow cytometry-based assay for autophagy. Autophagy. 6, 634-41 (2010),
  4. Frankel LB et al. microRNA-101 is a potent inhibitor of autophagy. EMBO J. 30, 4628-41 (2011),
  5. Gillis JM et al. Aminopeptidase-resistant peptides are targeted to lysosomes and subsequently degraded. Traffic. 12, 1897-910 (2011),
  6. Hartleben B et al. Autophagy influences glomerular disease susceptibility and maintains podocyte homeostasis in aging mice. J Clin Invest. 120, 1084-96 (2010),
  7. Ho H et al. WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition. J Biol Chem. 286, 12509-23 (2011),
  8. Joubert PE et al. Chikungunya virus-induced autophagy delays caspase-dependent cell death. J Exp Med. 209,1029-47 (2012),
  9. Kovacs JR et al. Autophagy promotes T-cell survival through degradation of proteins of the cell death machinery. Cell Death Differ. 19, 144-52 (2012),
  10. McKnight NC et al. Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC. EMBO J. 31, 1931-46 (2012),
  11. Moreau K et al. Autophagosome precursor maturation requires homotypic fusion. Cell. 146, 303-17 (2011),
  12. Morris CR et al. A knockout of the Tsg101 gene leads to decreased expression of ErbB receptor tyrosine kinases and induction of autophagy prior to cell death. PLoS One. 7, e34308 (2012),
  13. Saiki S et al. Caffeine induces apoptosis by enhancement of autophagy via PI3K/Akt/mTOR/p70S6K inhibition. Autophagy. 7, 176-87 (2011),
  14. Seto S et al. Coronin-1a inhibits autophagosome formation around Mycobacterium tuberculosis-containing phagosomes and assists mycobacterial survival in macrophages. Cell Microbiol. 14, 710-27 (2012),
  15. Shi CS et al. Activation of autophagy by inflammatory signals limits IL-1?A production by targeting ubiquitinated inflammasomes for destruction. Nat Immunol. 13, 255-63 (2012),
  16. Sims JJ et al. Polyubiquitin-sensor proteins reveal localization and linkage-type dependence of cellular ubiquitin signaling. Nat Methods. 9, 303-9 (2012),
  17. Starr T et al. Selective subversion of autophagy complexes facilitates completion of the Brucella intracellular cycle. Cell Host Microbe. 11, 33-45 (2012),
  18. Thayanidhi N et al. Alpha-synuclein delays endoplasmic reticulum (ER)-to-Golgi transport in mammalian cells by antagonizing ER/Golgi SNAREs. Mol Biol Cell. 21, 1850-63 (2010),
  19. Traver MK et al. Immunity-related GTPase M (IRGM) proteins influence the localization of guanylate-binding protein 2 (GBP2) by modulating macroautophagy. J Biol Chem. 286, 30471-80 (2011),
  20. Wang Z et al. Autophagy regulates myeloid cell differentiation by p62/SQSTM1-mediated degradation of PML-RARα oncoprotein. Autophagy. 7, 401-11 (2011)
  21. Ho, H., et al., J. Biol. Chem. 286, 12509-12523 (2011) [WB]
  22. Saiki, S., et al., Autophagy 7, 176-187 (2011) [WB]
  23. Kasai, M., et al., J. Immunol. 183, 7278-7285 (2009) [WB, IC, IHC]
  24. Mookerjee, S., et al., J. Neurosci. 29, 15134-15144 (2009) [IP, IHC]
  25. Nishitsuji, K., et al., Am. J. Pathol. 174, 957-969 (2009) [IC]
  26. Wang, Y., et al., J. Biol. Chem. 283, 4766-4777 (2008) [WB, IC]
  27. Wise JP Jr et al. Autophagy Disruptions Associated With Altered Optineurin Expression in Extranigral Regions in a Rotenone Model of Parkinson’s Disease. Front Neurosci. 12, 289 (2018)
  28. Kanda R et al. Expression of the glucagon-like peptide-1 receptor and its role in regulating autophagy in endometrial cancer. BMC Cancer 18, 657 (2018)
  29. Mori H et al. Induction of selective autophagy in cells replicating hepatitis C virus genome. J Gen Virol. 99, 1643-1657 (2018)
  30. Kajiume T, Kobayashi M, Human granulocytes undergo cell death via autophagy. Cell Death Discov. 4, 111 (2018)rnrn
  31. Zemirli N et al. The primary cilium protein folliculin is part of the autophagy signaling pathway to regulate epithelial cell size in response to fluid flow. Cell Stress. 3, 100-109 (2019)
  32. Vrahnas C et al. Author Correction: Increased autophagy in EphrinB2-deficient osteocytes is associated with elevated secondary mineralization and brittle bone. Nat Commun. 10, 5073 (2019)

References

  1. Kabeya, Y., et al., J. Cell Sci. 117, 2805-2812 (2004)
  2. Mizushima, N., et al., Mol. Biol. Cell 15, 1101-1111 (2004)
  3. Mizushima, N., et al., J. Cell Biol. 152, 657-667 (2001)
  4. Kabeya, Y., et al., EMBO J. 19, 5720-5728 (2000)