The antigen of the MBL Bion ANA substrate is a human epithelial cell (HEp-2) line. HEp-2 cells have been shown to have greater sensitivity than tissue sections and aid in sharper differential pattern recognition as well as detecting previously unreported nuclear antibodies.
The MBL Bion ANA (HEp-2) ANTIGEN SUBSTRATE SLIDES may be used as the antigenic substrate in indirect fluorescent antibody assays for the qualitative and/or semi-quantitative determination of antinuclear antibodies in human serum. MBL Bion ANA (HEp-2) ANTIGEN SUBSTRATE SLIDES are intended for use as an aid in the diagnosis of certain autoimmune diseases.
Antinuclear antibodies (ANA) are a group of autoantibodies characterized by specificity for numerous antigenic determinants of cell nuclei. While the role of ANA’s in the pathogenesis of autoimmune disease is controversial, they are quite useful as disease markers, primarily for diagnostic screening and also to monitor the course of connective tissue diseases. Because of the high correlation of positive antinuclear antibodies with SLE a negative ANA essentially rules out this disease. Although antibodies specific to DNA have a high correlation with SLE, antibodies to a number of other nuclear antigens appear to be of diagnostic and/or prognostic significance in diseases such as Progressive Systemic Sclerosis, Mixed Connective Tissue Disease, Sjogren's Syndrome, and Polymyositis; making ANA testing useful not only for SLE, but as a general screening tool for connective tissue diseases.
The MBL Bion ANA ANTIGEN SUBSTRATE SLIDES are individually foil wrapped slides of 16 wells with HEp-2 tissue culture cells fixed onto each well.
Antigen substrate slides used in indirect fluorescent antibody assays.