pFucci-S/G2/M Green-Hyg (Expression vector)



FUCCI (Fluorescent Ubiquitination-based Cell Cycle Indicator) is a set of fluorescent probes which enables the visualization of cell cycle progression in living cells. FUCCI utilizes the phase-dependent nature of replication licensing factors Cdt1 and Geminin. A fusion protein of a fragment of Cdt1 (amino acids 30-120) with the fluorescent protein monomeric Kusabira-Orange 2 (mKO2) serves as an indicator of G1 phase. A fusion protein of a fragment of Geminin (amino acids 1-110 or 1-60) with the fluorescent protein monomeric Azami-Green 1 (mAG1) visualizes the S,G2 and M phase. In summary, FUCCI utilizes the highly selective, rapid degradation of the replication licensing factors mediated by the ubiquitin proteasome system to give excellent visualizations of the cell cycle.

Target: pFucci-S/G2/M
Product Type: Vector
Size: 20 μg
Research Area / Disease: Fluorescent Proteins
Formulation: Dry form. Reconstitute with distilled water or TE before use.
Source: The CoralHue AG gene was cloned from a stony coral (Galaxea fascicularis).
Storage Temperature: -20°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.


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