pMitophagy Keima-Red mPark2 (Kan)
Mitophagy detection vectors are designed for the co-expression of MT-mKeima-Red. (CoralHue®Mitochondria-targeted monomeric Keima-Red) gene and mouse Parkin (mPark2) gene from the internal ribosome entry site (IRES), as part of a bicistronic transcript in mammalian cells. Keima-Red has been cloned from Montipara sp. A monomeric version of CoralHue®Keima-Red (mKeima-Red) displays a bimodal excitation spectrum with peaks at 440 and 586nm in neutral and acidic solutions, respectively, and an emission maximum at 620nm. Mitochondrial targeting of mKeima-Red is achieved by introducing a mitochondrial signal sequence at the N-terminus of mKeima-Red.
|Research Area / Disease:||Fluorescent Proteins|
|Regulatory Statement:||For Research Use Only. Not for use in diagnostic procedures.|
- Ma, Cui, et al. “Ubiquitinated AIF Is a Major Mediator of Hypoxia-Induced Mitochondrial Dysfunction and Pulmonary Artery Smooth Muscle Cell Proliferation.” Cell & Bioscience, vol. 12, no. 1, 2022, doi:10.1186/s13578-022-00744-3
- Ke, Po-Yuan, Chih-Wei Chang, and Yuan-Chao Hsiao. “Baicalein Activates Parkin-Dependent Mitophagy through NDP52 and OPTN.” Cells 11.7 (2022): 1132.