Afamin/Wnt3a CM is a product of JSR LIfe Sciences.
Wnt signaling is known to be involved in early development, maintenance and regeneration of stem cells, in cancer formation, and plays an important role in the growth and maintenance of these processes. In particular, Wnt3a has been revealed to be an essential niche component for maintaining the proliferation of Lgr5-positive stem cells in intestinal epithelial cells and is used for the production of various digestive organoids such as the small intestine, large intestine, stomach, pancreas and liver. Although Wnt3a has been conventionally used for the culture of gut organoids, it is a fat-soluble protein, so it forms aggregates in serum-free medium and can not exert its activity sufficiently. In 2016, Mihara et al. found that high Wnt3a activity can be maintained by forming a complex with Wnt3a by Afamin, which is one of the components of serum. In addition, by using Afamin and Wnt3a complex for organoid culture, long-term culture of organoid becomes possible.
1. E. Mihara, et al., Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin., eLife 5 (2016)2. K. Nanki, et al., Divergent Routes toward Wnt and R-spondin Niche independency during Human Gastric Carcinogenesis., Cell 174 (2018)3. S. Sugimoto, et al., Reconstruction of the Human Colon Epithelium In Vivo., Cell Stem Cell 22 (2018)4. T. Seino, et al., Human Pancreatic Tumor Organoids Reveal Loss of Stem Cell Niche Factor Dependence during Disease Progression., Cell Stem Cell 22 (2018)