The ST2 ELISA Kit measures soluble human ST2 protein by sandwich ELISA. The assay uses two monoclonal antibodies against two different epitopes of human ST2. Samples to be measured or standards are incubated in the microwells coated with anti-human ST2 monoclonal antibody. After washing, peroxidase-conjugated anti-human ST2 monoclonal antibody is added into the microwells and incubated. After another washing, the peroxidase substrate premixed with chromogen is added and allowed to incubate for an additional period of time. An acid solution is then added to each microwell to terminate the enzyme reaction and to stabilize the developed color. The optical density (O.D.) of each microwell is measured at 450 nm using a microplate reader. The concentration of human ST2 is calibrated from a dose response curve based on reference standards.
The ST2 ELISA Kit measures soluble human ST2 protein by sandwich ELISA.