ST2 ELISA Kit

  • Applications
    • ELISA
  • Code # 7638
  • Size 96 Wells
  • Price
    $513.71
Specifications

Background

The ST2 gene, also known as T1, Fit1, or DER4, was originally identified as a responsive gene that was highly induced by stimulation of various proliferation-inducing agents including serum, PDGF (platelet-derived growth factor), FGF (fibroblast growth factor), or lysophosphatidic acid in murine fibroblasts 1), 2). Three distinct forms of gene products have been reported and named ST2, ST2L, and ST2V. ST2 is a soluble secreted form of 37.1kD protein which lacks an intracellular domain, whereas ST2L is a transmembrane form of 61.5kD protein (the glycosylated forms of ST2 and ST2L are about 57kD and 80kD, respectively). This ST2L protein is very similar to IL-1R (interleukin-1 receptor) type I and II in structure, thus it is considered as a member of the IL-1R family 3), 4). ST2V, which is another novel variant form of human ST2, has been identified recently 5). ST2 proteins are expressed in a wide variety of types of human cells, including hematopoietic cells in various stages of differentiation, a population of the perIPheral blood mononuclear cells from healthy individuals, glioblastoma and astrocytoma cell lines, and colon cancer cells in addition to fibroblast cell lines 4). Thus ST2 proteins are considered to have some role in regulating cell growth or proliferation. On the other hand, the definitive functions of both ST2 proteins and their ligand molecule(s) which bind to ST2 proteins have remained unclear, though it has been reported that neither IL-1, or RA (receptor antagonist) binds to ST2 proteins, in spite of their structural similarity to IL-1R 3), 4). This indicates that ST2L protein is functionally independent from IL-1R. Furthermore, several studies have shown that ST2L is expressed on the cell surface of Th2 cells but not on the Th1 cells 4, 6), indicating the possibility that ST2L protein particIPates not only in the regulation of cell growth or proliferation, but also in the immune system including differentiation of T cells or immunological response via helper T cells. From these observations, ST2 proteins are considered to be one of the important proteins particIPating in various physiological phenomena. Thus, further analysis is required to understand their physiological functions.
  • Application:
    ELISA
  • Components:
    Microwells, Calibrator Powder, Detector Concentrate, Detector Diluent, Assay Diluent, Wash Concentrate, Substrate, Stop Solution
  • Description:

    The ST2 ELISA Kit measures soluble human ST2 protein by sandwich ELISA. The assay uses two monoclonal antibodies against two different epitopes of human ST2. Samples to be measured or standards are incubated in the microwells coated with anti-human ST2 monoclonal antibody. After washing, peroxidase-conjugated anti-human ST2 monoclonal antibody is added into the microwells and incubated. After another washing, the peroxidase substrate premixed with chromogen is added and allowed to incubate for an additional period of time. An acid solution is then added to each microwell to terminate the enzyme reaction and to stabilize the developed color. The optical density (O.D.) of each microwell is measured at 450 nm using a microplate reader. The concentration of human ST2 is calibrated from a dose response curve based on reference standards.

  • Product Type:
    ELISA Kit
  • Research Area:
    Cancer
  • Short Description:

    The ST2 ELISA Kit measures soluble human ST2 protein by sandwich ELISA.

  • Size:
    96 Wells
References
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  2. Mok, M. Y., et al., Rheumatology 49, 520-527 (2010)
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  5. Shimpo, M., et al., Circulation 109, 2186-2190 (2004)
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