MBLI ApoE4/Pan-ApoE ELISA Kit measures Human ApoE4 or Pan-ApoE by sandwich ELISA. The assay uses affinity purified polyclonal antibody against ApoE and monoclonal antibody against ApoE4.
In the wells coated with anti-ApoE polyclonal antibody, samples to be measured or standards are incubated. After washing, a peroxidase conjugated anti-ApoE4 monoclonal antibody or a peroxidase conjugated anti-ApoE polyclonal antibody is added in the microwell and incubated. After another washing, the peroxidase substrate is added and allowed to incubate for an additional period of time. An acid solution is then added to each well to terminate the enzyme reaction and to stabilize the developed color. The optical density (O.D.) of each well is then measured at 450 nm using a microplate reader. The concentration of ApoE4 or Pan-ApoE is calibrated from a standard curve based on reference standards.
MBLI ApoE4/Pan-ApoE ELISA Kit measures Human ApoE4 or Pan-ApoE by sandwich ELISA.