ApoE4/Pan-ApoE ELISA Kit

  • Applications
    • ELISA
  • Code # 7635
  • Size 96 Wells
  • Price
    $594.83
Specifications

Background

Apolipoprotein E (ApoE), a 35-kDa plasma protein containing sialic acid, constitutes a part of major apoproteins such as chylomicrons, VLDL and HDL. ApoE plays a role in the transport and metabolism of triglyceride-cholesterol, and is known to be synthesized in the liver, brain and other organs. ApoE is a polymorphic apolipoprotein exhibiting three major isoforms Apo E2, E3 and E4 coded by three alleles of ε2, ε3 and ε4 at a single gene locus respectively. A combination of these isoforms makes six different phenotypes (3 homozygotes-E2/E2, E3/E3, E4/E4 and 3 heterozygotes-E2/E3, E3/E4, E2/E4). ApoE2 is known as an important marker to diagnose type III hyperlipoproteinemia and is reported to be related to cardiac disease. ApoE4 is now in the spotlight as one of the risk factors for Alzheimer's disease. MBL ApoE4/Pan-ApoE ELISA Kit measures the amount of human ApoE4 or Pan-ApoE specifically with high sensitivity using affinity purified polyclonal antibody against ApoE and monoclonal antibody against ApoE4. It can also measure the difference among the homozygotes (E4/E4) and the heterozygotes (E2/E4, E3/E4) of ApoE4 phenotypes, and non-ApoE4 zygotes (E2/E2, E3/E3, E2/E3) by taking a concentration ratio between ApoE4 and Pan-ApoE.
  • Application:
    ELISA
  • Components:
    Microwells, Calibrator, Conjugate reagent (for ApoE4), Conjugate reagent (for Pan-ApoE), Conjugate diluent 1, Conjugate diluent 2, Assay diluent, Wash concentrate, Substrate, Stop solution, Instruction manual
  • Description:

    MBLI ApoE4/Pan-ApoE ELISA Kit measures Human ApoE4 or Pan-ApoE by sandwich ELISA. The assay uses affinity purified polyclonal antibody against ApoE and monoclonal antibody against ApoE4.

    In the wells coated with anti-ApoE polyclonal antibody, samples to be measured or standards are incubated. After washing, a peroxidase conjugated anti-ApoE4 monoclonal antibody or a peroxidase conjugated anti-ApoE polyclonal antibody is added in the microwell and incubated. After another washing, the peroxidase substrate is added and allowed to incubate for an additional period of time. An acid solution is then added to each well to terminate the enzyme reaction and to stabilize the developed color. The optical density (O.D.) of each well is then measured at 450 nm using a microplate reader. The concentration of ApoE4 or Pan-ApoE is calibrated from a standard curve based on reference standards.

  • Product Type:
    ELISA Kit
  • Research Area:
    Neuroscience
  • Short Description:

    MBLI ApoE4/Pan-ApoE ELISA Kit measures Human ApoE4 or Pan-ApoE by sandwich ELISA.

  • Size:
    96 Wells
References
  1. Mihovilovic, M., et al., J. Lipid Res. 48, 806-815 (2007)
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  5. Saunders, A. M., et al., Neurology 43, 1467-1472 (1993)
  6. Davignon, J., et al., Arterioscler. Thromb. Vasc. Biol. 8,1-21 (1988)
  7. Breslow, J. L., et al., J. Lipid Res. 23, 1124-1235 (1982)