Mouse IL-18 ELISA Kit

The Mouse IL-18 ELISA Kit is based on sandwich ELISA and capable of measuring mouse IL-18.



The Mouse Il-18 ELISA Kit is based on sandwich ELISA and is capable of measuring mouse Il-18.
This kit has a high sensitivity (25.0 pg/ml) and numerous citations.

Target: IL-18
Product Type: ELISA Kit
Size: 96 Wells
Application: ELISA
Research Area / Disease: Immunology


Microwell strips coated with anti-Mouse IL-18 antibody 8-well strip x 12 strips

Mouse IL-18 calibrator

Conjugate reagent (Peroxidase conjugate anti-mouse IL-18 monoclonal antibody)

Conjugate diluent (ready to use)

Assay diluent (ready to use)

Washing buffer

Substrate reagent (ready to use)

Stop solution (ready to use)


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  3. Li X et al. IL-18 binding protein (IL-18BP) as a novel radiation countermeasure after radiation exposure in mice. Sci Rep. 10, 18674 (2020)
  4. Tan S et al. H2 Protects Against Lipopolysaccharide-Induced Cardiac Dysfunction via Blocking TLR4-Mediated Cytokines Expression. Front Pharmacol. 10, 865 (2019)
  5. Wang J et al. Caspase-11-dependent pyroptosis of lung epithelial cells protects from melioidosis while caspase-1 mediates macrophage pyroptosis and production of IL-18. PLoS Pathog. 14, e1007105 (2018)
  6. de la Roche M et al. Trafficking of cholesterol to the ER is required for NLRP3 inflammasome activation. J Cell Biol. 217, 3560-3576 (2018)
  7. Banerjee S and Bond JS. Prointerleukin-18 is activated by meprin beta in vitro and in vivo in intestinal inflammation. J Biol Chem. 283, 31371-7 (2008)
  8. Rodriguez-Galan MC et al. Coexpression of IL-18 strongly attenuates IL-12-induced systemic toxicity through a rapid induction of IL-10 without affecting its antitumor capacity. J Immunol. 183, 740-8 (2009)
  9. Broz P et al. Redundant roles for inflammasome receptors NLRP3 and NLRC4 in host defense against Salmonella. J Exp Med. 207, 1745-55 (2010),
  10. Costa A et al. Activation of the NLRP3 inflammasome by group B streptococci. J Immunol. 188, 1953-60 (2012),
  11. Paget C et al. Role of γδ T cells in α-galactosylceramide-mediated immunity. J Immunol. 188, 3928-39 (2012)
  12. Grung P et al. Toll or Interleukin-1 Receptor (TIR) Domain-containing adaptor inducing interferon-  (TRIF)-mediated Caspase-11 protease production integrates Toll-like receptor 4 (TLR4) protein- and Nlrp3 inflammasome-mediated host defense against enteropathogens. J Biol Chem. 287, 34474-83 (2012),
  13. Meldrum KK et al. Profibrotic effect of interleukin-18 in HK-2 cells is dependent on stimulation of the Toll-like receptor 4 (TLR4) promoter and increased TLR4 expression. J Biol. Chem. 287, 40391-9 (2012),
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  25. Kuroda-morimoto M, Tanaka H, Hayashi N, et al. Contribution of IL-18 to eosinophilic airway inflammation induced by immunization and challenge with Staphylococcus aureus proteins. Int Immunol. 2010;22(7):561-70.
  26. Humann J, Lenz LL. Activation of naive NK cells in response to Listeria monocytogenes requires IL-18 and contact with infected dendritic cells. J Immunol. 2010;184(9):5172-8.
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  28. Hoshino T, Okamoto M, Sakazaki Y, Kato S, Young HA, Aizawa H. Role of proinflammatory cytokines IL-18 and IL-1beta in bleomycin-induced lung injury in humans and mice. Am J Respir Cell Mol Biol. 2009;41(6):661-70.
  29. Kim J, Shao Y, Kim SY, et al. Hypoxia-induced IL-18 increases hypoxia-inducible factor-1alpha expression through a Rac1-dependent NF-kappaB pathway. Mol Biol Cell. 2008;19(2):433-44.
  30. Reading PC, Whitney PG, Barr DP, et al. IL-18, but not IL-12, regulates NK cell activity following intranasal herpes simplex virus type 1 infection. J Immunol. 2007;179(5):3214-21.
  31. Zorrilla EP, Sanchez-alavez M, Sugama S, et al. Interleukin-18 controls energy homeostasis by suppressing appetite and feed efficiency. Proc Natl Acad Sci USA. 2007;104(26):11097-102.
  32. Wiersinga WJ, Wieland CW, Van der windt GJ, et al. Endogenous interleukin-18 improves the early antimicrobial host response in severe melioidosis. Infect Immun. 2007;75(8):3739-46.
  33. Hoshino T, Kato S, Oka N, et al. Pulmonary inflammation and emphysema: role of the cytokines IL-18 and IL-13. Am J Respir Crit Care Med. 2007;176(1):49-62.
  34. Qiao H, Sonoda KH, Ikeda Y, et al. Interleukin-18 regulates pathological intraocular neovascularization. J Leukoc Biol. 2007;81(4):1012-21.
  35. Neumann D, Tschernig T, Popa D, et al. Injection of IL-12- and IL-18-encoding plasmids ameliorates the autoimmune pathology of MRL/Mp-Tnfrsf6lpr mice: synergistic effect on autoimmune symptoms. Int Immunol. 2006;18(12):1779-87.
  36. Kuranaga N, Kinoshita M, Kawabata T, Habu Y, Shinomiya N, Seki S. Interleukin-18 protects splenectomized mice from lethal Streptococcus pneumoniae sepsis independent of interferon-gamma by inducing IgM production. J Infect Dis. 2006;194(7):993-1002.
  37. Ito H, Esashi E, Akiyama T, Inoue J, Miyajima A. IL-18 produced by thymic epithelial cells induces development of dendritic cells with CD11b in the fetal thymus. Int Immunol. 2006;18(8):1253-63.
  38. Takahashi HK, Watanabe T, Yokoyama A, et al. Cimetidine induces interleukin-18 production through H2-agonist activity in monocytes. Mol Pharmacol. 2006;70(2):450-3.
  39. Terada M, Tsutsui H, Imai Y, et al. Contribution of IL-18 to atopic-dermatitis-like skin inflammation induced by Staphylococcus aureus product in mice. Proc Natl Acad Sci USA. 2006;103(23):8816-21.
  40. Ino Y, Saeki Y, Fukuhara H, Todo T. Triple combination of oncolytic herpes simplex virus-1 vectors armed with interleukin-12, interleukin-18, or soluble B7-1 results in enhanced antitumor efficacy. Clin Cancer Res. 2006;12(2):643-52.
  41. Sasaki Y et al. IL-18 with IL-2 protects against Strongyloides venezuelensis infection by activating mucosal mast cell-dependent type 2 innate immunity. J Exp Med. 202, 607-16 (2005)
  42. Shirota H, Gursel I, Gursel M, Klinman DM. Suppressive oligodeoxynucleotides protect mice from lethal endotoxic shock. J Immunol. 2005;174(8):4579-83.
  43. Yajima T, Nishimura H, Saito K, Kuwano H, Yoshikai Y. Overexpression of Interleukin-15 increases susceptibility to lipopolysaccharide-induced liver injury in mice primed with Mycobacterium bovis bacillus Calmette-Guerin. Infect Immun. 2004;72(7):3855-62.
  44. Nakano H, Tsutsui H, Terada M, et al. Persistent secretion of IL-18 in the skin contributes to IgE response in mice. Int Immunol. 2003;15(5):611-21.
  45. Hedtjärn M et al. Interleukin-18 involvement in hypoxic-ischemic brain injury. J Neurosci. (2002)
  46. Konishi H, et al. IL-18 contributes to the spontaneous development of atopic dermatitis-like inflammatory skin lesion independently of IgE/stat6 under specific pathogen-free conditions. Proc Natl Acad Sci USA. 2002;99(17):11340-5.
  47. Hoshino T, et al IL-18-transgenic mice: in vivo evidence of a broad role for IL-18 in modulating immune function. J Immunol. 2001;166(12):7014-8.
  48. Habu Y, et al. The mechanism of a defective IFN-gamma response to bacterial toxins in an atopic dermatitis model, NC/Nga mice, and the therapeutic effect of IFN-gamma, IL-12, or IL-18 on dermatitis. J Immunol. 2001;166(9):5439-47.
  49. Seki E, Tsutsui H, Nakano H, et al. Lipopolysaccharide-induced IL-18 secretion from murine Kupffer cells independently of myeloid differentiation factor 88 that is critically involved in induction of production of IL-12 and IL-1beta. J Immunol. 2001;166(4):2651-7.


  1. Anthony DA, et al., J Immunol, 185, 1794 (2010)
  2. Banerjee S, et al., J Biol Chem, 283, 31371 (2008)
  3. Broz P, et al., J Exp Med, 207, 1745 (2010)
  4. Chen GY, et al., J Immunol, 186, 7187 (2011)
  5. Costa A, et al., J Immunol, 188, 1953 (2012)
  6. Gurung P, et al., J Biol Chem, 287, 34474 (2012)
  7. Habu Y, et al., J Immunol, 166, 5439 (2001)
  8. Hitzler I, et al., J Immunol, 188, 3594 (2012)
  9. Hoshino T, et al., Am J Respir Cell Mol Biol, 41, 661 (2009)
  10. Hoshino T, et al., Am J Respir Crit Care Med, 176, 49 (2007)
  11. Hoshino T, et al., J Immunol, 166, 7014 (2001)
  12. Humann J, et al., J Immunol, 184, 5172 (2010)
  13. Ino Y, et al., Clin Cancer Res, 12, 643 (2006)
  14. Ito H, et al., Int Immunol, 18, 1253 (2006)
  15. Kim J, et al., Mol Biol Cell, 19, 433 (2008)
  16. Kimura K, et al., J Leukoc Biol, 89, 433 (2011)
  17. Konishi H, et al., PNAS, 99, 11340 (2002)
  18. Kuranaga N, et al., J Infect Dis, 194, 993 (2006)
  19. Kuroda-Morimoto M, et al., Int Immunol, 22, 561 (2010)
  20. Li H, et al., J Immunol, 183, 1528 (2009)
  21. Liu Z, et al., J Biol Chem, 287, 16955 (2012)
  22. Mattarollo SR, et al., Blood, 120, 3019 (2012)
  23. McPhee JB, et al., Infect Immun, 78, 3529 (2010)
  24. Meldrum KK, et al., J Biol Chem, 287, 40391 (2012)
  25. Nagarajan UM, et al., J Immunol, 188, 2866 (2012)
  26. Nakano H, et al., Int Immunol, 15, 611 (2003)
  27. Neumann D, et al., Int Immunol, 18, 1779 (2006)
  28. Paget C, et al., J Immunol, 188, 3928 (2012)
  29. Qiao H, et al., J Leukoc Biol, 81, 1012 (2007)
  30. Reading PC, et al., J Immunol, 179, 3214 (2007)
  31. Rodriguez-Galan MC, et al., J Immunol, 183, 740 (2009)
  32. Sasaki Y, et al., J Exp Med, 202, 607 (2005)
  33. Seki E, et al., J Immunol, 166, 2651 (2001)
  34. Shirota H, et al., J Immunol, 174, 4579 (2005)
  35. Srinivasan G, et al., J Immunol, 189, 1911 (2012)
  36. Takahashi HK, et al., Mol Pharmacol, 70, 450 (2006)
  37. Terada M, et al., PNAS, 103, 8816 (2006)
  38. Tsai PY, et al., Am J Physiol Renal Physiol, 301, F751 (2011)
  39. Wiersinga WJ, et al., Infect Immun, 75, 3739 (2007)
  40. Yajima T, et al., Infect Immun, 72, 3855 (2004)
  41. Zorrilla EP, et al., PNSA, 104, 11097 (2007)
Code 7625
Product Type ELISA Kit
Size 96 Wells
Application ELISA