Mouse IL-18 ELISA Kit

  • Applications
    • ELISA
  • Code # 7625
  • Size 96 Wells
  • Price


Interleukin 18 (IL-18) is an 18 kDa cytokine which is identified as a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock. It shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1β converting enzyme (ICE, caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It has been reported that IL-18 is produced from dendritic cells, activated macrophages, Kupffer cells, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalons. IFN- is produced by activated T and NK cells and plays critical roles in the defense against microbial pathogens. IFN-γ activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens expression, and inhibits osteoclast activation. IL-18 acts on T helper 1-type (Th1) cells, and in combination with IL-12 strongly induces production of IFN-γ by these cells. Pleiotropic effects of IL-18 have also been reported, including enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells, enhancement of Fas ligand expression by Th1 cells. The “Human IL-18 ELISA Kit” is a useful reagent for specifically measuring human IL-18 with high sensitivity by ELISA. This Kit does not detect 1 ng/mL of various cytokines, such as human IFN-α, IFN-γ, IL-1β, IL-4, IL-5, IL-6, IL-10, IL-12, GM-CSF and murine IL-18. The results were all bellow the detection limit of 12.5 pg/mL.
  • Application:
  • Components:
    Microwell strips coated with anti-Mouse IL-18 antibody 8-well strip x 12 strips, Mouse IL-18 calibrator (Lyophilized) 2 vials, Conjugate reagent (Peroxidase conjugate anti-mouse IL-18 monoclonal antibody) (x101) 0.2 ml x 1 vial, Conjugate diluent (ready to use) 24 ml x1 vial, Assay diluent (ready to use) 30 ml x 1 vial, Washing buffer (powder) 3 packages, Substrate reagent (TMB/H2O2) (ready to use) 15 ml x 1 vial, Stop solution (2N H2SO4) (ready to use) (irritant) 18 ml x1 vial
  • Description:
    The Human Il-18 ELISA Kit is based on sandwich ELISA and is capable of measuring mouse Il-18. This kit has a high sensitivity (25.0 pg/ml) and has over 65 citations.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Product Type:
    ELISA Kit
  • Sensitivity:
    25.0 pg/ml
  • Short Description:
    The Mouse IL-18 ELISA Kit is based on sandwich ELISA and capable of measuring mouse IL-18.
  • Size:
    96 Wells
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  6. Paget C et al. Role of γδ T cells in α-galactosylceramide-mediated immunity. J Immunol. 188, 3928-39 (2012)
  7. Grung P et al. Toll or Interleukin-1 Receptor (TIR) Domain-containing adaptor inducing interferon-  (TRIF)-mediated Caspase-11 protease production integrates Toll-like receptor 4 (TLR4) protein- and Nlrp3 inflammasome-mediated host defense against enteropathogens. J Biol Chem. 287, 34474-83 (2012),
  8. Meldrum KK et al. Profibrotic effect of interleukin-18 in HK-2 cells is dependent on stimulation of the Toll-like receptor 4 (TLR4) promoter and increased TLR4 expression. J Biol. Chem. 287, 40391-9 (2012),
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  10. Gurung P, Malireddi RK, Anand PK, et al. Toll or interleukin-1 receptor (TIR) domain-containing adaptor inducing interferon-β (TRIF)-mediated caspase-11 protease production integrates Toll-like receptor 4 (TLR4) protein- and Nlrp3 inflammasome-mediated host defense against enteropathogens. J Biol Chem. 2012;287(41):34474-83.
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  33. Takahashi HK, Watanabe T, Yokoyama A, et al. Cimetidine induces interleukin-18 production through H2-agonist activity in monocytes. Mol Pharmacol. 2006;70(2):450-3.
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  1. Anthony DA, et al., J Immunol, 185, 1794 (2010)
  2. Banerjee S, et al., J Biol Chem, 283, 31371 (2008)
  3. Broz P, et al., J Exp Med, 207, 1745 (2010)
  4. Chen GY, et al., J Immunol, 186, 7187 (2011)
  5. Costa A, et al., J Immunol, 188, 1953 (2012)
  6. Gurung P, et al., J Biol Chem, 287, 34474 (2012)
  7. Habu Y, et al., J Immunol, 166, 5439 (2001)
  8. Hitzler I, et al., J Immunol, 188, 3594 (2012)
  9. Hoshino T, et al., Am J Respir Cell Mol Biol, 41, 661 (2009)
  10. Hoshino T, et al., Am J Respir Crit Care Med, 176, 49 (2007)
  11. Hoshino T, et al., J Immunol, 166, 7014 (2001)
  12. Humann J, et al., J Immunol, 184, 5172 (2010)
  13. Ino Y, et al., Clin Cancer Res, 12, 643 (2006)
  14. Ito H, et al., Int Immunol, 18, 1253 (2006)
  15. Kim J, et al., Mol Biol Cell, 19, 433 (2008)
  16. Kimura K, et al., J Leukoc Biol, 89, 433 (2011)
  17. Konishi H, et al., PNAS, 99, 11340 (2002)
  18. Kuranaga N, et al., J Infect Dis, 194, 993 (2006)
  19. Kuroda-Morimoto M, et al., Int Immunol, 22, 561 (2010)
  20. Li H, et al., J Immunol, 183, 1528 (2009)
  21. Liu Z, et al., J Biol Chem, 287, 16955 (2012)
  22. Mattarollo SR, et al., Blood, 120, 3019 (2012)
  23. McPhee JB, et al., Infect Immun, 78, 3529 (2010)
  24. Meldrum KK, et al., J Biol Chem, 287, 40391 (2012)
  25. Nagarajan UM, et al., J Immunol, 188, 2866 (2012)
  26. Nakano H, et al., Int Immunol, 15, 611 (2003)
  27. Neumann D, et al., Int Immunol, 18, 1779 (2006)
  28. Paget C, et al., J Immunol, 188, 3928 (2012)
  29. Qiao H, et al., J Leukoc Biol, 81, 1012 (2007)
  30. Reading PC, et al., J Immunol, 179, 3214 (2007)
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  32. Sasaki Y, et al., J Exp Med, 202, 607 (2005)
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  36. Takahashi HK, et al., Mol Pharmacol, 70, 450 (2006)
  37. Terada M, et al., PNAS, 103, 8816 (2006)
  38. Tsai PY, et al., Am J Physiol Renal Physiol, 301, F751 (2011)
  39. Wiersinga WJ, et al., Infect Immun, 75, 3739 (2007)
  40. Yajima T, et al., Infect Immun, 72, 3855 (2004)
  41. Zorrilla EP, et al., PNSA, 104, 11097 (2007)