Human IL-18 ELISA Kit

Human IL-18 ELISA Kit



The Human Il-18 ELISA Kit is based on sandwich ELISA and is capable of measuring human Il-18. This kit has a high sensitivity (12.5 pg/ml) and continues to be gather multiple citations every year.

With regard to diseases, high levels of IL-18 are detected in the blood of patients with allergic diseases (bronchial asthma, atopic dermatitis, allergic rhinitis) and those with autoimmune diseases (rheumatoid arthritis, systemic lupus erythematosus [SLE], adult Still’s disease, multiple sclerosis, Chrohn’s disease, ulcerative colitis). IL-18 is also found in the urine of patients with acute renal disorder and appears to mediate the progression of type 2 diabetes.

Target: IL-18
Product Type: ELISA Kit
Size: 96 Wells
Application: ELISA
Research Area / Disease: Immunology
Species Reactivity: Human
Gene ID Human:


Gene ID Mouse:


Sensitivity: 12.5 pg/ml
Storage Temperature: 4°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.


Microwell strips coated with anti-Human IL-18 antibody 8-well strip – 12strips

Human IL-18 calibrator (Lyophilized)

Conjugate reagent (Peroxidase conjugate anti-Human IL-18 monoclonal antibody)

Conjugate diluent (ready to use)

Assay diluent (ready to use)

Wash concentrate ( x 10)

Substrate reagent (ready to use)

Stop solution (ready to use)


Okamoto M et al. Evaluation of interferon-gamma, interferon-gamma-inducing cytokines, and interferon-gamma-inducible chemokines in tuberculous pleural effusions. J Lab Clin Med. 145, 88-93 (2005)

Wakiguchi H et al. Successful control of juvenile dermatomyositis-associated macrophage activation syndrome and interstitial pneumonia: distinct kinetics of interleukin-6 and -18 levels. Pediatr Rheumatol. 13, 49 (2015)

Chiba A et al. Activation status of mucosal-associated invariant T cells reflects disease activity and pathology of systemic lupus erythematosus. Arthritis Res Ther. 19, 58 (2017)

Lou J et al. MiR-20b inhibits mycobacterium tuberculosis induced inflammation in the lung of mice through targeting NLRP3. Exp Cell Res. 358, 120-128 (2017)

C Weel I et al. Increased expression of NLRP3 inflammasome in placentas from pregnant women with severe preeclampsia. J Reprod Immunol. 123, 40-47 (2017)

Gibbs S et al. Assessment of metal sensitizer potency with the reconstructed human epidermis IL-18 assay. Toxicology 393, 62-72 (2018)

Corsini E et al. NCTC 2544 and IL-18 production: a tool for the identification of contact allergens. Toxicol In Vitro. (2013)

Galbiati V et al. Establishment of an in vitro photoallergy test using NCTC2544 cells and IL-18 production. Toxicol In Vitro. (2013)

Bae S, Kim H, Lee N, et al. α-Enolase expressed on the surfaces of monocytes and macrophages induces robust synovial inflammation in rheumatoid arthritis. J Immunol. 2012;189(1):365-72.

Uratsuji H, Tada Y, Kawashima T, et al. P2Y6 receptor signaling pathway mediates inflammatory responses induced by monosodium urate crystals. J Immunol. 2012;188(1):436-44.

Denoble AE, Huffman KM, Stabler TV, et al. Uric acid is a danger signal of increasing risk for osteoarthritis through inflammasome activation. Proc Natl Acad Sci USA. 2011;108(5):2088-93.

Luan Y, Guo Y, Li S, et al. Interleukin-18 among atrial fibrillation patients in the absence of structural heart disease. Europace. 2010;12(12):1713-8.

Hall IE, Yarlagadda SG, Coca SG, et al. IL-18 and urinary NGAL predict dialysis and graft recovery after kidney transplantation. J Am Soc Nephrol. 2010;21(1):189-97.

Harada M, Obara K, Hirota T, et al. A functional polymorphism in IL-18 is associated with severity of bronchial asthma. Am J Respir Crit Care Med. 2009;180(11):1048-55.

Alsaleh G, Suffert G, Semaan N, et al. Bruton’s tyrosine kinase is involved in miR-346-related regulation of IL-18 release by lipopolysaccharide-activated rheumatoid fibroblast-like synoviocytes. J Immunol. 2009;182(8):5088-97.

Porazko T, Kúzniar J, Kusztal M, et al. IL-18 is involved in vascular injury in end-stage renal disease patients. Nephrol Dial Transplant. 2009;24(2):589-96.

Piccini A, Carta S, Tassi S, Lasiglié D, Fossati G, Rubartelli A. ATP is released by monocytes stimulated with pathogen-sensing receptor ligands and induces IL-1beta and IL-18 secretion in an autocrine way. Proc Natl Acad Sci USA. 2008;105(23):8067-72.

Thompson SR, Mccaskie PA, Beilby JP, et al. IL18 haplotypes are associated with serum IL-18 concentrations in a population-based study and a cohort of individuals with premature coronary heart disease. Clin Chem. 2007;53(12):2078-85.

Thompson SR, Novick D, Stock CJ, et al. Free Interleukin (IL)-18 levels, and the impact of IL18 and IL18BP genetic variation, in CHD patients and healthy men. Arterioscler Thromb Vasc Biol. 2007;27(12):2743-9.

Washburn KK, Zappitelli M, Arikan AA, et al. Urinary interleukin-18 is an acute kidney injury biomarker in critically ill children. Nephrol Dial Transplant. 2008;23(2):566-72.

Bachmann M, Dragoi C, Poleganov MA, Pfeilschifter J, Mühl H. Interleukin-18 directly activates T-bet expression and function via p38 mitogen-activated protein kinase and nuclear factor-kappaB in acute myeloid leukemia-derived predendritic KG-1 cells. Mol Cancer Ther. 2007;6(2):723-31.

Sailer CA, Pott GB, Dinarello CA, et al. Whole-blood interleukin-18 level during early HIV-1 infection is associated with reduced CXCR4 coreceptor expression and interferon- gamma levels. J Infect Dis. 2007;195(5):734-8.

Ye Q, Chen B, Tong Z, et al. Thalidomide reduces IL-18, IL-8 and TNF-alpha release from alveolar macrophages in interstitial lung disease. Eur Respir J. 2006;28(4):824-31.

Vujisic S, Lepej SZ, Emedi I, Bauman R, Remenar A, Tiljak MK. Ovarian follicular concentration of IL-12, IL-15, IL-18 and p40 subunit of IL-12 and IL-23. Hum Reprod. 2006;21(10):2650-5.

Takahashi HK, Watanabe T, Yokoyama A, et al. Cimetidine induces interleukin-18 production through H2-agonist activity in monocytes. Mol Pharmacol. 2006;70(2):450-3.

Coward WR, Marei A, Yang A, Vasa-nicotera MM, Chow SC. Statin-induced proinflammatory response in mitogen-activated peripheral blood mononuclear cells through the activation of caspase-1 and IL-18 secretion in monocytes. J Immunol. 2006;176(9):5284-92.

Krogh-madsen R, Plomgaard P, Møller K, Mittendorfer B, Pedersen BK. Influence of TNF-alpha and IL-6 infusions on insulin sensitivity and expression of IL-18 in humans. Am J Physiol Endocrinol Metab. 2006;291(1):E108-14.

Parikh CR, Abraham E, Ancukiewicz M, Edelstein CL. Urine IL-18 is an early diagnostic marker for acute kidney injury and predicts mortality in the intensive care unit. J Am Soc Nephrol. 2005;16(10):3046-52.

Niyonsaba F et al. The human beta-defensins (-1, -2, -3, -4) and cathelicidin LL-37 induce IL-18 secretion through p38 and ERK MAPK activation in primary human keratinocytes. J Immunol. (2005)

Mazodier K, Marin V, Novick D, et al. Severe imbalance of IL-18/IL-18BP in patients with secondary hemophagocytic syndrome. Blood. 2005;106(10):3483-9.

Skurk T, Kolb H, Müller-scholze S, Röhrig K, Hauner H, Herder C. The proatherogenic cytokine interleukin-18 is secreted by human adipocytes. Eur J Endocrinol. 2005;152(6):863-8.

Yamagami H, Kitagawa K, Hoshi T, et al. Associations of serum IL-18 levels with carotid intima-media thickness. Arterioscler Thromb Vasc Biol. 2005;25(7):1458-62.

Hodara VL, Velasquillo MC, Parodi LM, Giavedoni LD. Expression of CD154 by a simian immunodeficiency virus vector induces only transitory changes in rhesus macaques. J Virol. 2005;79(8):4679-90.

Hung J, Mcquillan BM, Chapman CM, Thompson PL, Beilby JP. Elevated interleukin-18 levels are associated with the metabolic syndrome independent of obesity and insulin resistance. Arterioscler Thromb Vasc Biol. 2005;25(6):1268-73.

Takahashi HK, Mori S, Iwagaki H, et al. Differential effect of LFA703, pravastatin, and fluvastatin on production of IL-18 and expression of ICAM-1 and CD40 in human monocytes. J Leukoc Biol. 2005;77(3):400-7.

Kitasato Y, Hoshino T, Okamoto M, et al. Enhanced expression of interleukin-18 and its receptor in idiopathic pulmonary fibrosis. Am J Respir Cell Mol Biol. 2004;31(6):619-25.

Oku H, Tsuji Y, Kashiwamura SI, et al. Role of IL-18 in pathogenesis of endometriosis. Hum Reprod. 2004;19(3):709-14.

Kaizu M, Ami Y, Nakasone T, et al. Higher levels of IL-18 circulate during primary infection of monkeys with a pathogenic SHIV than with a nonpathogenic SHIV. Virology. 2003;313(1):8-12.

Bas S, Kvien TK, Buchs N, Fulpius T, Gabay C. Lower level of synovial fluid interferon-gamma in HLA-B27-positive than in HLA-B27-negative patients with Chlamydia trachomatis reactive arthritis. Rheumatology (Oxford). 2003;42(3):461-7.

Ahmad R, Sindhu ST, Toma E, Morisset R, Ahmad A. Elevated levels of circulating interleukin-18 in human immunodeficiency virus-infected individuals: role of peripheral blood mononuclear cells and implications for AIDS pathogenesis. J Virol. 2002;76(24):12448-56.

Rouabhia M, Ross G, Pagé N, Chakir J. Interleukin-18 and gamma interferon production by oral epithelial cells in response to exposure to Candida albicans or lipopolysaccharide stimulation. Infect Immun. 2002;70(12):7073-80.

Cho D et al. The enhanced IL-18 production by UVB irradiation requires ROI and AP-1 signaling in human keratinocyte cell line (HaCaT). Biochem Biophys Res Commun. (2002)

Sugawara S et al. Neutrophil proteinase 3-mediated induction of bioactive IL-18 secretion by human oral epithelial cells. J Immunol. (2001)

Vankayalapati R, Wizel B, Lakey DL, et al. T cells enhance production of IL-18 by monocytes in response to an intracellular pathogen. J Immunol. 2001;166(11):6749-53.

Shida K, Shiratori I, Matsumoto M, et al. An alternative form of IL-18 in human blood plasma: complex formation with IgM defined by monoclonal antibodies. J Immunol. 2001;166(11):6671-9.

Narita M, Tanaka H, Abe S, Yamada S, Kubota M, Togashi T. Close association between pulmonary disease manifestation in Mycoplasma pneumoniae infection and enhanced local production of interleukin-18 in the lung, independent of gamma interferon. Clin Diagn Lab Immunol. 2000;7(6):909-14.

Vankayalapati R, Wizel B, Weis SE, Samten B, Girard WM, Barnes PF. Production of interleukin-18 in human tuberculosis. J Infect Dis. 2000;182(1):234-9.

Kohka H, Nishibori M, Iwagaki H, et al. Histamine is a potent inducer of IL-18 and IFN-gamma in human peripheral blood mononuclear cells. J Immunol. 2000;164(12):6640-6.

Flisiak-Jackiewicz M et al. Predictive Role of Interleukin-18 in Liver Steatosis in Obese Children. Can J Gastroenterol Hepatol. 2018, 3870454 (2018)

Pietrzak D et al. Serum concentrations of interleukin 18 and 25-hydroxyvitamin D3 correlate with depression severity in men with psoriasis. PLoS One 13, e0201589 (2018)

Bil W et al. Comparison of the skin sensitization potential of 3 red and 2 black tattoo inks using interleukin-18 as a biomarker in a reconstructed human skin model. Contact Dermatitis. 79, 336-345 (2018)

Furuya MY et al. Tofacitinib inhibits granulocyte-macrophage colony-stimulating factor-induced NLRP3 inflammasome activation in human neutrophils. Arthritis Res Ther. 20, 196 (2018)

Colasanti T et al. Diesel exhaust particles induce autophagy and citrullination in Normal Human Bronchial Epithelial cells. Cell Death Dis. 9, 1073 (2018)

Semino C et al. Progressive waves of IL-1β release by primary human monocytes via sequential activation of vesicular and gasdermin D-mediated secretory pathways. Cell Death Dis. 9, 1088 (2018)

Limandjaja GC et al. Reconstructed human keloid models show heterogeneity within keloid scars. Arch Dermatol Res. 310 815-826 (2018)

Leu WJ et al. Extract From Plectranthus amboinicus Inhibit Maturation and Release of Interleukin 1β Through Inhibition of NF-κB Nuclear Translocation and NLRP3 Inflammasome Activation. Front Pharmacol. 10, 573 (2019)


  1. Alsaleh, G., et al., J. Immunol. 182, 5088-5097 (2009)
  2. Thompson, S. R., et al., Clin. Chemist. 53, 2078-2085 (2007)
  3. Omoto, Y., et al., J. Immunol. 177, 8315-8319 (2006)
  4. Vujisic, S., et al., Hum. Reprod. 21, 2650-2655 (2006)
  5. Parikh, C. R., et al., J. Am. Soc. Nephrol. 16, 3046-3052 (2005)
  6. Tada, H., et al., Infect. Immunol. 73, 7967-7976 (2005)
  7. Baratin, M. L., et al., PNAS. 102, 14747-14752 (2005)
  8. Oku, H., et al., Hum. Reprod. 19, 709-714 (2004)
  9. Kaizu, M., et al., Virology 313, 8-12 (2003)
  10. Ahmad, R., et al., J. Virol. 76, 12448-12456 (2002)
  11. Rouabhia, M., et al., Infect. Immunol. 75, 3739-3746 (2002)
  12. Shida, K., et al., J. Immunol. 166, 6671-6679 (2001)
  13. Narita, M., et al., Clin. Diagn. Lab. 7, 909-914 (2000)
  14. Tao, D., et al., Cell Immunol. 173, 230-235 (1998)
  15. Taniguchi, M., et al., J. Immunol. Methods 206, 107-113 (1997)
  16. Micallef, M., et al., Eur. J. Immunol. 26, 1647-1651 (1996)
  17. Ushio, S., et al., J. Immunol. 156, 4274-4279 (1996)
  18. Okamura, H., et al., Nature 378, 88-91 (1995)