MESACUP ECP TEST (RUO)

  • Applications
    • ELISA
  • Code # 7618E
  • Size 96 wells
  • Price Call for Price
Specifications

Background

The MESACUP ECP TEST measures human ECP by sandwich ELISA. This ELISA detects human ECP with a minimum detection limit of 0.125 ng/ml and does not cross-react with EDN. In the wells coated with anti-human ECP monoclonal antibody, samples to be measured or standards are incubated. After washing, a peroxidase conjugated anti-human ECP polyclonal antibody is added into the microwells and incubated. After another washing, the peroxidase substrate is mixed with the chromogen and allowed to incubate for an additional period of time. An acid solution is then added to each well to terminate the enzyme reaction and to stabilize the developed color. The optical density (O.D.) of each well is then measured at 450 nm using a microplate reader.The concentration of ECP is calibrated from a standard curve based on reference standards.

  • Application:
    ELISA
  • Components:
    • Microwell strips coated with anti-human ECP antibody: 8-well x 12 strips
    • ECP standard (ready to use): 6 concs. 0.75 mL each
    • Assay diluent (ready to use) 20 mL: 1 vial
    • Conjugate reagent: Horseradish Peroxidase conjugated anti-human ECP polyclonal antibody, ready to use, 12 mL: 1 via
    • Wash concentrate, 10 x concentrated solution, 100 mL: 1 vial
    • Substrate reagent TMB/H2O2, ready to use 20 mL: 1 vial
    • Stop solution 0.5 mol/L H2SO4, ready to use 20 mL: 1 vial
    • Positive control: 0.75 mL x 1 vial
    • Negative control: 0.75 mL x 1 vial
  • Description:

    MESACUP ECP TEST  is the reagent for measuring specifically with high sensitivity ECP by ELISA. The MESACUP ECP TEST measures human ECP by sandwich ELISA. It detects human ECP with a minimum detection limit of 0.125 ng/ml and does not cross-react with EDN.

    For Research Use Only. Not for use in diagnostic procedures.

  • Product Type:
    ELISA Kit
  • Research Area:
    Autoimmune
  • Short Description:

    INTENDED USE

    The MESACUP ECP TEST is quantitative assay kit for human ECP in serum and urine by sandwich ELISA. 

    For Research Use Only. Not for use in diagnostic procedures.

  • Size:
    96 wells
References
  1. Gleich, G., et al., Proc. Natl. Acad. Sci. USA, 83, 3146-3150 (1986)
  2. Krisyjansson, S., et al., Annals of medicine 28, 395-399 (1996)
  3. Peterson, C., et al., Eur. J Haematol., 40, 415-423 (1988)
  4. Zimmerman, B., et al., Clin Exp Allergy, 23, 564-570 (1993)