Anti-GFP (Green Fluorescent Protein) pAb (Polyclonal Antibody)

Specifications:

Background

Since the detection of intracellular Aequorea Victria Green Fluorescent Protein (GFP) requires only irradiation by UV or blue light, it provides an excellent means for monitoring gene expression and protein localization in living cells. The polyclonal anti-GFP antibody can detect GFP and its variants on Western blotting, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry.

Description

Polyclonal antibody argeting GFP for ICC, IHC, IPP, ChIP, WB, EM.

This GFP antibody reacts with GFP, EBFP, SEBFP, ECFP, SECFP, EGFP, SEGFP, cpSEGFP, EYFP, Venus, cpVenus, R-pericam, and Sapphire.

Target: GFP
Product Type: Antibody
Size: 100 µl
Application: ChIP, EM, ICC, IHC, IP, WB
Research Area / Disease: Epitope Tag
Conjugate: Unlabeled
Antibody Type: Polyclonal
Formulation: 100 µL volume of PBS containing 50% glycerol, pH7.2. No preservative is contained.
Isotype: IgG
Immunogen: Recombinant GFP
Host Species: Rabbit
Source: This antibody was purified from rabbit serum using ion exchange chromatography. The rabbit was immunized with recombinant GFP protein. The reactivity with recombinant partner and other E. coli components are absorbed using affinity chromatographic techniq
Reactivity: This antibody reacts with GFP on Western blotting, Immunoprecipitation, and Immunocytochemistry. This antibody also detects GFP-tagged proteins expressed in mammalian cell on Western blotting, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry.
Storage Temperature: -20°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Citations

Trinh DA et al. Inhibitor of Growth 4 (ING4) is a positive regulator of rRNA synthesis.rnSci Rep. 9, 17235 (2019)
Matsumura K et al. Autism-associated protein kinase D2 regulates embryonic cortical neuron development.Biochem Biophys Res Commun. 519, 626-632 (2019)
Mori S et al. Self-organized formation of developing appendages from murine pluripotent stem cells. Nat Commun. 10, 3802 (2019)t
Shirahase T et al. Ethanol Preference and Drinking Behavior Are Controlled by RNA Editing in the Nucleus Accumbens. Front Behav Neurosci. 12, 331 (2019)
Watanabe K et al. The Cep57-pericentrin module organizes PCM expansion and centriole engagement. Nat Commun. 10, 931 (2019)
Tsugama D et al. Protein phosphatase 2A regulates the nuclear accumulation of the Arabidopsis bZIP protein VIP1 under hypo-osmotic stress. J Exp Bot. 70, 6101-6112 (2019)
Cao J et al. RACK1 Promotes Self-Renewal and Chemoresistance of Cancer Stem Cells in Human Hepatocellular Carcinoma through Stabilizing Nanog. Theranostics. 9, 811-828 (2019)
Kiuchi T et al. Two CCCH-type zinc finger domains in the Masc protein are dispensable for masculinization and dosage compensation in Bombyx mori. Insect Biochem Mol Biol. 104, 30-38 (2019)
Duan GF et al. Signal peptide represses GluK1 surface and synaptic trafficking through binding to amino-terminal domain. Nat Commun. 9, 4879 (2018)rn
Shimauchi-Ohtaki H et al. The dynamics of revascularization after white matter infarction monitored in Flt1-tdsRed and Flk1-GFP mice. Neurosci Lett. 692, 70-76 (2019)
Tanaka J et al. Generation of orthotopically functional salivary gland from embryonic stem cells. Nat Commun. 9, 4216 (2018)
Plein A et al. Erythro-myeloid progenitors contribute endothelial cells to blood vessels. Nature 562, 223-228 (2018)
Bin Saifullah MA et al. Cell type-specific activation of mitogen-activated protein kinase in D1 receptor-expressing neurons of the nucleus accumbens potentiates stimulus-reward learning in mice. Sci Rep. 8, 14413 (2018)
Sugiyama Y et al. Intravenous Administration of Bone Marrow-Derived Mesenchymal Stem Cell, but not Adipose Tissue-Derived Stem Cell, Ameliorated the Neonatal Hypoxic-Ischemic Brain Injury by Changing Cerebral Inflammatory State in Rat. Front Neurol. 9, 757 (2018)
Wang X et al. Girdin/GIV regulates collective cancer cell migration by controlling cell adhesion and cytoskeletal organization. Cancer Sci. 109, 3643-3656 (2018)
Matsumoto A et al. Detection of SUN1 Splicing Variants at the mRNA and Protein Levels in Cancer. Methods Mol Biol. 1840, 307-319 (2018)
Arii J et al. ESCRT-III mediates budding across the inner nuclear membrane and regulates its integrity. Nat Commun. 9, 3379 (2018)
Wang H et al. A potato STRUBBELIG-RECEPTOR FAMILY member, StLRPK1, associates with StSERK3A/BAK1 and activates immunity. J Exp Bot. 69, 5573-5586 (2018)
Kondo T et al. Calcium Transient Dynamics of Neural Ensembles in the Primary Motor Cortex of Naturally Behaving Monkeys. Cell Rep. 24, 2191-2195.e4. (2018)
Hisamoto N et al. Phosphatidylserine exposure mediated by ABC transporter activates the integrin signaling pathway promoting axon regeneration. Nat Commun. 9, 3099 (2018)
Nabeshima R et al. Loss of Fam60a, a Sin3a subunit, results in embryonic lethality and is associated with aberrant methylation at a subset of gene promoters. Elife 7, e3643 (2018)
Yang L et al. Reconstituting Arabidopsis CRY2 Signaling Pathway in Mammalian Cells Reveals Regulation of Transcription by Direct Binding of CRY2 to DNA. Cell Rep. 24, 585-593.e4(2018)
Ojima N et al. Genetic dissection of stress-induced reproductive arrest in Drosophila melanogaster females. PLoS Genet. 14, e1007434 (2018)
Kendall GC et al. AX3-FOXO1 transgenic zebrafish models identify HES3 as a mediator of rhabdomyosarcoma tumorigenesis. Elife 7, e33800 (2018)
Tsuchiya M et al. Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation. Nat Commun. 9, 2049 (2018)
Naderi S, et al., cAMP-induced degradation of cyclin D3 through association with GSK-3beta. J Cell Sci. 117:3769-83. (2004)
Ertesvag A, et al., Vitamin A potentiates CpG-mediated memory B-cell proliferation and differentiation: involvement of early activation of p38MAPK. Blood. 109:3865-72. (2007),
Engedal N, et al., All-trans retinoic acid stimulates IL-2-mediated proliferation of human T lymphocytes: early induction of cyclin D3. J Immunol. 177:2851-61. (2006),
Code 598
Product Type Antibody
Size 100 µl
Application ChIP, EM, ICC, IHC, IP, WB
Price
$307.04
Qty: