Anti-HA-tag pAb-Agarose

  • Applications
    • IP
  • Target HA
  • Host Species Rabbit
  • Code # 561-8
  • Size 400 μl
  • Price
    $289.63
Specifications

Background

Epitope tagging has widely been accepted technique that fuse an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow; YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VSV-G-Tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.
  • Antibody Type:
    Polyclonal
  • Application:
    IP
  • Conjugate:
    Agarose
  • Description:
    Polyclonal antibody of 400 μl targeting HA for IPP.
  • Formulation:
    540 mg of anti-HA Tag polyclonal antibody covalently coupled to 200 mL of agarose gel and provided as a 50% gel slurry suspended in PBS containing preservative (0.09% sodium azide) for a total volume of 400 mL.
  • Host Species:
    Rabbit
  • Immunogen:
    YPYDVPDYA (HA-tag)-KLH
  • Isotype:
    IgG
  • Product Type:
    Antibody
  • Reactivity:
    This antibody recognizes HA-tagpeptide sequence (YPYDVPDYA) onImmunoprecipitation.
  • Research Area:
    Epitope Tag
  • Short Description:
    HA tag Polyclonal Antibody.
  • Size:
    400 μl
  • Storage Temperature:
    4°C
  • Target:
    HA
Citations
  1. Cillessen SA, et al., Inhibition of the intrinsic apoptosis pathway downstream of caspase-9 activation causes chemotherapy resistance in diffuse large B-cell lymphoma. Clin Cancer Res. 1, 7012-21. 2007,
  2. Ferreira CG, et al., Assessment of IAP (inhibitor of apoptosis) proteins as predictors of response to chemotherapy in advanced non-small-cell lung cancer patients. Ann Oncol. 12, 799-805. 2001,
  3. Ferreira CG, et al., Expression of X-linked inhibitor of apoptosis as a novel prognostic marker in radically resected non-small cell lung cancer patients. Clin Cancer Res.7, 2468-74. 2001,
  4. Marsden VS, et al., Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death. Blood. 107, 1872-7. 2006,
  5. Muris JJ, et al., Immunohistochemical profiling of caspase signaling pathways predicts clinical response to chemotherapy in primary nodal diffuse large B-cell lymphomas. Blood. 105, 2916-23. 2005,
  6. Silke J, et al., Determination of cell survival by RING-mediated regulation of inhibitor of apoptosis (IAP) protein abundance. Proc Natl Acad Sci U S A. 102, 16182-7. 2005,
  7. Silke J, et al., Unlike Diablo/smac, Grim promotes global ubiquitination and specific degradation of X chromosome-linked inhibitor of apoptosis (XIAP) and neither cause apoptosis. J Biol Chem. 279, 4313-21. 2004,
  8. Tanaka H, et al., 3-Phosphoinositide-dependent protein kinase-1-mediated IkappaB kinase beta (IkkB) phosphorylation activates NF-kappaB signaling. J Biol Chem. 280, 40965-73. 2005