MethylHunter MBD1-based Methylated DNA Enrichment Kit

  • Target MBD1
  • Code # 5270
  • Size 25 Tests
  • Price
    $486.68
Specifications

Background

MBD1 belongs to the family of MBD (methyl-CpG binding domain)-containing proteins and binds to methylated CpG DNA through its N-terminus MBD. MethylHunter MBD1-based Methylated DNA Enrichment Kit can enrich DNA fragments containing methylated CpG DNA by using recombinant His-tagged MBD1. Efficiency to enrich methylated DNA fragments is higher than other methods such as antibody-based enrichment methods or other MBD protein- based methods. Enrichment bias is lower than one that might be observed in other MBD protein- based methods. Enriched methylated DNA fragments can be readily used for following Microarray, Real-time PCR, and Next Generation Sequencer analysis.
  • Components:
    MBD1 protein 0.75 mg/mL 200 _l, Control Genomic DNA Mouse genomic DNA, sonicated (100 ng/_l) 20 _l, Positive Control Primers 10 _M each, for methylated DNA amplification 20 _l, Negative Control Primers 10 _M each, for un-methylated DNA amplification 20 _l, 10x MBD buffer 100 mM Tris-HCl (pH 8.0), 0.2% Triton X-100 800 _l, 50% Glycerol 800 _l, 10x Wash Buffer 10x MBD buffer, 50% Glycerol, 2 M NaCl 15 mL, Elute Buffer 1x MBD buffer, 5% Glycerol, 0.2 M NaCl, 0.5 M imidazole 6 mL
  • Description:
    MethylHunter MBD1-based Methylated DNA Enrichment Kit can enrich DNA fragments containing methylated CpG DNA by using recombinant His-tagged MBD1.Enriched methylated DNA fragments can be readily used for following Microarray, Real-time PCR, and Next Generation Sequencer analysis.
  • Product Type:
    Kit
  • Short Description:
    MBD1-based Methylated DNA Enrichment Kit.
  • Size:
    25 Tests
  • Storage Temperature:
    -20°C. Please refer to datasheet for additional information
  • Target:
    MBD1
References
  1. Fujita, N., et. al., Mol. Cell Biol. 19, 6415-6426 (1999)
  2. Ohki, I., et. al., EMBO J. 18, 6653-6661 (1999)
  3. Fujita, N., et. al., Mol. Cell Biol. 20, 5107-5118 (2000)
  4. Ohki, I., et. al., Cell 105, 487-497 (2001)
  5. Fraga, M. F., et. al., Nucleic Acid Res. 31, 1765-1774 (2003)