Recombinant tagged proteins are widely used for protein purification and protein characterization. There are many purification methods and kits available. DDDDK epitope tag (DYKDDDDK) has been widely used as a multi-purpose tag for protein purification.
MBLI’s DDDDK-tagged Protein PURIFICATION CARTRIDGE is ready-to-use-cartridge for the isolation of DDDDK-tagged protein from cell culture supernatants and cell lysate under neutral pH condition. The cartridge can be used directly on automated ÄKTA FPLC Systems. The elution of DDDDK-tagged proteins from the cartridge is achieved by the addition of the DDDDK-tag peptide (DYKDDDDK). As the DDDDK-tag peptide competes with DDDDK-tagged proteins on the cartridge, the purified DDDDK-tagged proteins maintain function and retain protein activity.