Solutions for Life Science & Diagnostics
Anti-LC3 pAb
Additional Information
Anti-LC3 pAb Specifications
| Alternative Names: | microtubule-associated protein 1 light chain 3 beta microtubule-associated protein 1 light chain 3 alpha microtubule-associated protein 1 light chain 3 gamma |
| Background: | Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I (18 kDa). A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II (16 kDa). This antibody can detect both forms of LC3. |
| Clonality: | Polyclonal |
| Formulation: | In PBS/50% glycerol, pH 7.2 |
| Gene ID Human: | 84557 |
| Gene ID Mouse: | 66734 |
| Host Species: | Rabbit |
| Immunogen: | recombinant Human LC3 (MAP1LC3B:1-120 a.a.) |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
| Isotype: | IgG |
| Product Type: | Primary Antibody |
| Shipping: | 4 |
| Size: | 100 µl |
| Source: | This antibody was purified from rabbit serum using protein A agarose. The rabbit was immunized with the recombinant human LC3 [MAP1LC3B (1-120 aa)]. |
| Species Reactivities: | Human, Mouse, Rat, Hamster |
| Status: | RUO |
| Storage: | -20℃ |
| Target: | LC3 |
Applications: FCM, IC, IH, IP, WB
| FCM: | 1:200 |
| ICC: | 1:500-1:1000 |
| IHC: | 1:1000-1:2000 |
| IPP: | 2 μL/300 μL of cell extract from 1x107 |
| WB: | 1:1000 |
1) Tabata, K., et al., Mol. Biol. Cell 21, 4162-4172 (2010) 2) Kiyono, K., et al., Cancer Res. 69, 8844-8852 (2009) 3) Saitoh, T., et al., Nature 456, 264-268 (2008) 4) Wan, G., et al., J. Biol. Chem. 283, 21540-21549 (2008) 5) Ohneet, Y., et al., J. Biol
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