Solutions for Life Science & Diagnostics
Anti-S-tag pAb-Agarose
Additional Information
Anti-S-tag pAb-Agarose Specifications
| Background: | Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. S-Tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. S-Tag can combine with S-protein to form functional RNase S, allowing detection and quantification of S-Tag fusion proteins by enzymatic assays. However, antibody against the S-Tag sequence provides a more convenient and flexible method to easily identify, detect, or purify S-Tag containing fusion proteins. |
| Clonality: | Polyclonal |
| Conjugate: | Agarose |
| Formulation: | 360 mg of anti-S-Tag polyclonal antibody covalently coupled to 200 mL of agarose gel and provided as a 50% gel slurry suspended in PBS containing preservative (0.09% sodium azide) for a total volume of 400 mL. |
| Host Species: | Rabbit |
| Immunogen: | Carrier protein conjugated synthetic peptide (KETAAAKFERQHIDSC) |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
| Isotype: | IgG |
| Product Type: | Primary Antibody |
| Reactivity: | This antibody recognizes S-Tag peptide sequence (KETAAAKFERQHIDS) on Immunoprecipitation. |
| Shipping: | 4 |
| Size: | 400 uL |
| Source: | This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with carrier protein (CP) conjugated synthetic peptide, CP -KETAAAKFERQHIDS. |
| Status: | RUO |
| Storage: | 4℃ |
| Target: | S |
Applications: IP
| IPP: | 20 μL of gel slurry |
There are no references for Anti-S-tag pAb-Agarose at this time.
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