Anti-S-tag pAb-Agarose Specifications
|Background:||Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged proteinâ€™s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. S-Tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. S-Tag can combine with S-protein to form functional RNase S, allowing detection and quantification of S-Tag fusion proteins by enzymatic assays. However, antibody against the S-Tag sequence provides a more convenient and flexible method to easily identify, detect, or purify S-Tag containing fusion proteins.
|Formulation:||360 mg of anti-S-Tag polyclonal antibody covalently coupled to 200 mL of agarose gel and provided as a 50% gel slurry suspended in PBS containing preservative (0.09% sodium azide) for a total volume of 400 mL.|
|Immunogen:||Carrier protein conjugated synthetic peptide (KETAAAKFERQHIDSC)|
|Regulatory Statement:||For Research Use Only. Not for use in diagnostic procedures.|
|Product Type:||Primary Antibody|
|Reactivity:||This antibody recognizes S-Tag peptide sequence (KETAAAKFERQHIDS) on Immunoprecipitation.|
|Source:||This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with carrier protein (CP) conjugated synthetic peptide, CP -KETAAAKFERQHIDS.|
There are no references for Anti-S-tag pAb-Agarose at this time.