| Background: | Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. The DDDDK epitope tag peptide sequence (DYKDDDDK) was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. The DDDDK peptide has been widely used as a multi-purpose tag, and anti-DDDDK antibodies are optimally suited for identifying, detecting, purifying, and monitoring the expression levels of recombinant DDDDK fusion proteins. |
| Clonality: | Polyclonal |
| Formulation: | PBS/50% glycerol, pH7.2 |
| Host Species: | Rabbit |
| Immunogen: | KLH-conjugated synthetic peptide , DYKDDDDK |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
| Isotype: | IgG |
| Product Type: | Primary Antibody |
| Shipping: | 4 |
| Size: | 100 µg |
| Status: | RUO |
| Storage: | -20℃ |
| Target: | DDDDK |
Applications: IC, IP, WB
| ICC: | 1:1000 |
| IPP: | 5 μL/sample |
| WB: | 1:1000 |
There are no references for Anti-DDDDK-tag pAb at this time.
