Mouse β5t polyclonal antibody : Trial Size
Code No: PD021B
Datasheet:
FID:5727 | PATH:sites/default/files/datasheets/PD021B.PDF
Background:
The 20S proteasome is composed of 28 subunits, arranged as a cylinder-shaped structure made of four stacked hetero-heptameric rings: 1-7, 1-7, 1-7, and 1-7. 1, 2, and 5 function the catalytic subunit for proteolysis. Murata and colleagues recently identified a new catalytic subunit called 5t, which is only expressed in thymic cortical epithelial cells and dominantly incorporated into thymic 20S proteasomes. The integration of 5t into proteasomes reduces their chymotrypsin-like activity responsible for producing high affinity peptides for MHC class I. The depletion of 5t in mice resulted in defective development of CD8 single positive T cells in the thymus, indicating that 5t has a key role for generating the MHC class I-restricted CD8+T cell repertoire during thymic selection. There results challenge the 15 years old theory of T-cell development, particularly positive selection in the thymus and the phenomenon of peripheral tolerance.
Product Type:
Primary Antibody
Species Reactivities:
Mouse
References:
1) Murata, S., et al., Science 316, 1349-1353 (2007)
2) Hirano, Y., et al., Nature 437, 1381-1385 (2005)
Reactivity:
This antibody reacts with mouse ?5t on Western blotting and Immunohistochemistry. This antibody specifically stains the epithelial cells of the thymic cortex by Immunocytochemistry. As marker for cortical thymic epithelial cells, ?5t is superior in specificity to Ly51, the established marker of these cells.
Intended Use:
For Research use only. Not for use in diagnostic procedure.
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