proteins synthesized in the endoplasmic reticulum (ER). GM130 is a member of the golgin family of coiled-coil proteins that localizes predominantly to the cis-Golgi. GM130 might participate in ER-Golgi traffic.

SOURCE: This antibody was purified from hybridoma (clone 5G8) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with the synthetic peptide corresponding to C-terminal of human GM130.

FORMULATION: 100 g IgG in 100 L volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

STORAGE: This antibody solution is stable for one year from the date of purchase when stored at -20oC.

REACTIVITY: This antibody reacts with human GM130 on Western blotting, Immunoprecipitation, and Immunocytochemistry.

APPLICATIONS:
Western blotting; 1 g/mL for chemiluminescence detection system
Immunoprecipitation; 2 g/300 L of cell extract from
3 x 106 cells
Immunohistochemistry; Not tested
Immunocytochemistry; 5 g/mL
Flow cytometry; Not tested

Detailed procedure is provided in the following PROTOCOLS.

SPECIES CROSS REACTIVITY:
Species Human Mouse Rat
Cells Hela, 293T
A549 Not Tested Not Tested
Reactivity on WB +

INTENDED USE:
For Research Use Only. Not for use in diagnostic procedures.

REFERENCES:
1) Diao, A., et al., J. Biol. Chem. 283, 6957-6967 (2008)
2) Alvarez, C., et al., J. Biol. Chem. 276, 2693-2700 (2001)