Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Rubicon was identified as Beclin1 interacting protein. Three distinct Beclin1 complexes exist in cells, one of the complexes including Rubicon (Beclin1, hVps34, hVps15, UVRAG, Rubicon) down regulates the process of autophagosome maturation and endocytosis.