Solutions for Life Science & Diagnostics
Anti-Atg3 mAb
Additional Information
Anti-Atg3 mAb Specifications
| Background: | Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Microtubule-associated protein light chain 3 (LC3) is a homologue of yeast Atg8, an essential component of autophagy. Following synthesis, the C-terminus of LC3 is cleaved by a cysteine protease-Atg4, to produce LC3-I, which is located in cytosolic fraction. LC3-I is activated by the E1-like enzyme Atg7 and forms a Atg7-LC3-I thioester. Atg7-LC3-I is transferred to Atg3 to form Atg3-LC3-I thioester. Atg3 is an E2-like enzyme that catalyzes the conjugation of LC3-I and phosphatidylethanolamine (PE) to form LC3-II. The LC3-II-PE conjugate is essential for binding tightly to autophagosomal membrane. |
| Clonality: | Monoclonal |
| Clone Number: | 3E8 |
| Formulation: | 1 mg/mL in PBS/50% glycerol, pH 7.2 |
| Gene ID Human: | 64422 |
| Gene ID Mouse: | 67841 |
| Host Species: | Mouse |
| Immunogen: | Recombinant Human Atg3 |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
| Isotype: | IgG2b |
| Product Type: | Primary Antibody |
| Shipping: | 4 |
| Size: | 100 μg |
| Species Reactivities: | Human, Mouse, Rat, Hamster |
| Status: | RUO |
| Storage: | -20℃ |
| Target: | Atg3 |
Applications: IC, IP, WB
| ICC: | 0.5 μg/mL |
| IPP: | 2.5 μg/300 μL of cell extract from 3x106 |
| WB: | 1 μg/mL |
There are no references for Anti-Atg3 mAb at this time.
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