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Mcm2 Monoclonal Antibody : Trial Size

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Code No: M069-3S
Datasheet: 
application/pdf iconM069-3S.pdf
FID:5558 | PATH:sites/default/files/datasheets/M069-3S.PDF
Gene/antigen ID number: 
4171
Target: 

MCM 2

Background: 

Minichromosome Maintenance (MCM) proteins play an essential role in eukaryotic DNA replication. Six MCM proteins (MCM2-7) have been identified from yeast to mammals. In human cells, MCM4, -6, and -7 proteins form a stable complex, and MCM2 is loosely associated with this complex. The heterotrimeric MCM complex containing MCM4, -6 and -7 has DNA helicase activity, and such activity can be inhibited by the presence of MCM2. Cdc7/Dbf4 protein kinase, which is required for the initiation of DNA replication phosphorylates MCM2 in vitro and in vivo. From these observations, it is thought that phosphorylation of MCM2 may remove MCM2 from the MCM4, -6, and -7 complex to activate its DNA helicase activity at the onset of DNA replication.

Size: 
10 µg
Application: 
WB
Application: 
IC
Clone Number: 
E38
Conjugate: 
protein A Sepharose
Product Type: 
Primary Antibody
Host Species: 
Mouse
Species Reactivities: 
Human
Species Reactivities: 
Mouse
Species Reactivities: 
Rat
Isotype: 
IgG1
Storage Temp. (°C): 
4
Shipping Temp. (°C): 
4
Pictures: 
Mcm2 Monoclonal Antibody : Trial Size
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Detailed procedure is provided in the following PROTOCOLS.
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K0084-3S Anti-p14ARF (DCS-240)
K0085-3S Anti-Cdh1 (DCS-266)
K0087-3S Anti-Chk2 (DCS-270)
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References: 

1) James, P. J., et al. Nature. 375, 418-421 (19

2) Nakatsuru, S., et al. Cytogenet Cell Genet. 68, 226-230 (1995)
3) Ishimi, Y., et al. J. Biol. Chem. 271, 24115-24122 (1

4) Sato, N., et al. EMBO J. 16, 4340-4351 (1997)
5) Ishimi, Y., et al. J. Biol. Chem. 273, 8369-8375 (1998)
6) Wei, J., et al. EMBO J. 18, 5703-5713 (1999) 996) 95)

Reactivity: 
This antibody detects ~120 kDa of Human MCM2 protein from whole cell lysate of human cell lines, such as Jurkat, HeLa and HL60 on Western blotting.
Intended Use: 
For Research use only. Not for use in diagnostic procedure.
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