Solutions for Life Science & Diagnostics
Anti-GFP (Green Fluorescent Protein) mAb
Additional Information
Anti-GFP (Green Fluorescent Protein) mAb Specifications
| Background: | Green Fluorescent Protein (GFP) tagging provide an excellent means for monitoring gene expression and protein localization in living cells, so that it is widely accepted by molecular and cell biological research. Monoclonal anti-GFP antibody can detect GFP and its variants on Western blotting, Immunoprecipitation and Immunocytochemistry. |
| Clonality: | Monoclonal |
| Clone Number: | 1E4 |
| Formulation: | 1 mg/mL in PBS/50% glycerol, pH 7.2 |
| Gene ID: | 7011691 |
| Host Species: | Mouse |
| Immunogen: | Recombinant fulll length GFP (246 a.a.) |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
| Isotype: | IgG2b |
| Product Type: | Primary Antibody |
| Reactivity: | This antibody reacts with GFP on Western blotting, Immunoprecipitation and Immunocytochemistry. It reacts with EBFP, SEBFP, ECFP, SECFP, EGFP, SEGFP cpSEGFP, EYFP, Venus, cpVenus, R-pericam, and Sapphire. |
| Shipping: | 4 |
| Size: | 100 µl |
| Source: | This antibody was purified from hybridoma (clone 1E4) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with recombinant full length GFP protein (246 aa). |
| Status: | RUO |
| Storage: | -20℃ |
| Target: | GFP |
Applications: IC, IH, IP, WB
| ICC: | 2 μg/mL |
| IHC: | 10 μg/mL |
| IPP: | 5 μg/sample |
| WB: | 1 μg/mL |
1) Asada, N. and Sanada, K., J. Neurosci. 30, 8852-8865 (2010) 2) Banerjee, S., et al., Neuron 64, 871-884 (2009) 3) Komuro, A., et al., J. Natl. Cancer Inst. 101, 592-604 (2009) 4) Nakanishi, M., et al., J. Biol. Chem. 282, 22993-23004 (2007) 5) Takeda,
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