Solutions for Life Science & Diagnostics
Anti-PML (Human) mAb
Additional Information
Anti-PML (Human) mAb Specifications
| Background: | Acute promyelocytic leukemia (APL) is associated with a t(15;17) translocation that creates the promyelocyte-retinoic acid receptor ï¡ (PML-RARï¡) fusion protein and successfully differentiated by all-trans-retinoic acid (ATRA). PML-RARï¡ consists of all amino acid of RARï¡ except the first 59 amino acids and includes its DNA-binding and ligand-binding domains. PML-RARï¡ contains the functional domains of PML which includes the DNA binding and dimerization property. Thus, the functions of PML and/or retinoid X receptor are sequestrated by PML-RARï¡ in a dominant negative manner. In APL cells, the PML-RARï¡ and PML are immunologically localized as microgranules in the nuclei and cytoplasm, whereas in normal cells, PML is immunologically found as a discrete speckled pattern in nuclei. The ATRA treatment of the APL cells triggers a reorganization of PML to generate normal localization. Anti-PML antibody is a strong tool for the detection of the chromosomal translocation t(15;17) on the APL cells and/or determination of the sensitivity of the APL cells to the ATRA differentiation of hematopoietic cells and apoptosis. |
| Clonality: | Monoclonal |
| Clone Number: | 1B9 |
| Formulation: | 1 mg/mL in PBS/50% glycerol, pH 7.2 |
| Gene ID Human: | 5371 |
| Gene ID Mouse: | 18854 |
| Host Species: | Mouse |
| Immunogen: | Recombinant Human fulll length PML |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
| Isotype: | IgG1 |
| Product Type: | Primary Antibody |
| Shipping: | 4 |
| Size: | 100 μg |
| Species Reactivities: | Human |
| Status: | RUO |
| Storage: | -20℃ |
| Target: | PML |
Applications: FCM, IC, IP, WB
| FCM: | 10 ug/mL |
| ICC: | 1 ug/mL |
| IPP: | Optimal Dilution to be determined by end user. |
| WB: | 1 ug/mL |
There are no references for Anti-PML (Human) mAb at this time.
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