Solutions for Life Science & Diagnostics
Anti-BAX (Human) mAb
Additional Information
Anti-BAX (Human) mAb Specifications
| Background: | Bax (Bcl-associated X protein) is a 21 kDa tumor suppressor protein that suppresses tumorigenesis and stimulates apoptosis in vivo. Bax has extensive amino acid homology to Bcl-2. It can homodimerize through its BH3 domain and it forms heterodimers with other Bcl-2 family members through its BH1 and BH2 domains. Overexpression of Bax promotes apoptosis and counters the death repressor activity of Bcl-2 and Bcl-xL. It is believed that the ratio of Bcl-2/Bax complexes to free protein controls the relative susceptibility of cells to death stimuli. Apoptotic stimuli cause the translocation of monomeric Bax from the cytosol to the mitochondria where it forms Bax homodimers. Localization of Bax to the mitochondria results in the activation of caspase-3, membrane blebbing, and nuclear fragmentation. Bax also induces mitochondrial dysfunction by increasing mitochondrial membrane permeability. It accelerates the opening of the mitochondrial porin channel VDAC, thus regulating the release of cytochrome c during apoptosis. |
| Clonality: | Monoclonal |
| Clone Number: | 4F11 |
| Formulation: | This antibody is lyophilized form. Prepare a stock solution by dissolving the lyophilized antibody in 100 mL of distilled water. After reconstitution, the IgG concentration should be 1 mg/mL in PBS (pH 7.2) containing 1% sucrose. No preservative is contai |
| Gene ID Human: | 581 |
| Gene ID Mouse: | 12028 |
| Host Species: | Mouse |
| Immunogen: | Recombinant Human BAXprotein |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
| Isotype: | IgG2b k |
| Product Type: | Primary Antibody |
| Reactivity: | This antibody reacts with human BAX (24 kDa) on Western blotting. |
| Shipping: | 4 |
| Size: | 100 μg |
| Source: | This antibody was purified from hybridoma (clone 4F11) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with recombinant human BAX. |
| Species Reactivities: | Human |
| Status: | RUO |
| Storage: | 4℃ |
| Target: | Bax |
Applications: FCM, IC, IH, IP, WB
| FCM: | 5-10 μg/mL (final concentration) |
| ICC: | 10 μg/mL |
| IHC: | 10 μg/mL (Heat treatment is necessary for paraffin |
| IPP: | 5-10 μg /200 μL of cell extract from 5x106 |
| WB: | 0.1-1 μg/mL |
1) Cartron, P-F., et al., J. Biol. Chem. 280, 10587-10598 (2005) 2) Starczynski, J., et al., J. Clin. Oncol. 23, 1514-1521 (2005) 3) Suárez, L., et al., Clin. Cancer Res. 10, 7599-7606 (2004) 4) Utsunomiya, K., et al., J. Histochem. Cytochem. 52, 805-812
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